Physicochemical Properties and Effects of Honeys on Key Biomarkers of Oxidative Stress and Cholesterol Homeostasis in HepG2 Cells
Manuka honey and newly developed honeys (arjuna, guggul, jiaogulan and olive) were examined for their physicochemical, biochemical properties and effects on oxidative stress and cholesterol homeostasis in fatty acid-induced HepG2 cells. The honeys exhibited standard moisture content (<20%), elect...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-01-01
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| Series: | Nutrients |
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| Online Access: | https://www.mdpi.com/2072-6643/13/1/151 |
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| author | Huong Thi Lan Nguyen Stefan Kasapis Nitin Mantri |
| author_facet | Huong Thi Lan Nguyen Stefan Kasapis Nitin Mantri |
| author_sort | Huong Thi Lan Nguyen |
| collection | DOAJ |
| description | Manuka honey and newly developed honeys (arjuna, guggul, jiaogulan and olive) were examined for their physicochemical, biochemical properties and effects on oxidative stress and cholesterol homeostasis in fatty acid-induced HepG2 cells. The honeys exhibited standard moisture content (<20%), electrical conductivity (<0.8 mS/cm), acidic pH, and monosaccharides (>60%), except olive honey (<60% total monosaccharides). They all expressed non-Newtonian behavior and 05 typical regions of the FTIR spectra as those of natural ones. Guggul and arjuna, manuka honeys showed the highest phenolic contents, correlating with their significant antioxidant activities. Arjuna, guggul and manuka honeys demonstrated the agreement of total cholesterol reduction and the transcriptional levels of <i>AMPK</i>, <i>SREBP2</i>, <i>HCMGR</i>, <i>LDLR</i>, <i>LXRα</i>. Jiaogulan honey showed the least antioxidant content and activity, but it was the most cytotoxic. Both jiaogulan and olive honeys modulated the tested gene in the pattern that should lead to a lower TC content, but this reduction did not occur after 24 h. All 2% concentrations of tested honeys elicited a clearer effect on <i>NQO1</i> gene expression. In conclusion, the new honeys complied with international norms for natural honeys and we provide partial evidence for the protective effects of manuka, arjuna and guggul honeys amongst the tested ones on key biomarkers of oxidative stress and cholesterol homeostasis, pending further studies to better understand their modes of action. |
| first_indexed | 2024-03-10T13:29:15Z |
| format | Article |
| id | doaj.art-a269667375bd49dea7646106fc03ceb9 |
| institution | Directory Open Access Journal |
| issn | 2072-6643 |
| language | English |
| last_indexed | 2024-03-10T13:29:15Z |
| publishDate | 2021-01-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Nutrients |
| spelling | doaj.art-a269667375bd49dea7646106fc03ceb92023-11-21T08:17:09ZengMDPI AGNutrients2072-66432021-01-0113115110.3390/nu13010151Physicochemical Properties and Effects of Honeys on Key Biomarkers of Oxidative Stress and Cholesterol Homeostasis in HepG2 CellsHuong Thi Lan Nguyen0Stefan Kasapis1Nitin Mantri2The Pangenomics Lab, School of Science, RMIT University, Melbourne, VIC 3083, AustraliaThe Pangenomics Lab, School of Science, RMIT University, Melbourne, VIC 3083, AustraliaThe Pangenomics Lab, School of Science, RMIT University, Melbourne, VIC 3083, AustraliaManuka honey and newly developed honeys (arjuna, guggul, jiaogulan and olive) were examined for their physicochemical, biochemical properties and effects on oxidative stress and cholesterol homeostasis in fatty acid-induced HepG2 cells. The honeys exhibited standard moisture content (<20%), electrical conductivity (<0.8 mS/cm), acidic pH, and monosaccharides (>60%), except olive honey (<60% total monosaccharides). They all expressed non-Newtonian behavior and 05 typical regions of the FTIR spectra as those of natural ones. Guggul and arjuna, manuka honeys showed the highest phenolic contents, correlating with their significant antioxidant activities. Arjuna, guggul and manuka honeys demonstrated the agreement of total cholesterol reduction and the transcriptional levels of <i>AMPK</i>, <i>SREBP2</i>, <i>HCMGR</i>, <i>LDLR</i>, <i>LXRα</i>. Jiaogulan honey showed the least antioxidant content and activity, but it was the most cytotoxic. Both jiaogulan and olive honeys modulated the tested gene in the pattern that should lead to a lower TC content, but this reduction did not occur after 24 h. All 2% concentrations of tested honeys elicited a clearer effect on <i>NQO1</i> gene expression. In conclusion, the new honeys complied with international norms for natural honeys and we provide partial evidence for the protective effects of manuka, arjuna and guggul honeys amongst the tested ones on key biomarkers of oxidative stress and cholesterol homeostasis, pending further studies to better understand their modes of action.https://www.mdpi.com/2072-6643/13/1/151honeyphytochemicalsantioxidantscholesteroloxidative stressgene expression |
| spellingShingle | Huong Thi Lan Nguyen Stefan Kasapis Nitin Mantri Physicochemical Properties and Effects of Honeys on Key Biomarkers of Oxidative Stress and Cholesterol Homeostasis in HepG2 Cells Nutrients honey phytochemicals antioxidants cholesterol oxidative stress gene expression |
| title | Physicochemical Properties and Effects of Honeys on Key Biomarkers of Oxidative Stress and Cholesterol Homeostasis in HepG2 Cells |
| title_full | Physicochemical Properties and Effects of Honeys on Key Biomarkers of Oxidative Stress and Cholesterol Homeostasis in HepG2 Cells |
| title_fullStr | Physicochemical Properties and Effects of Honeys on Key Biomarkers of Oxidative Stress and Cholesterol Homeostasis in HepG2 Cells |
| title_full_unstemmed | Physicochemical Properties and Effects of Honeys on Key Biomarkers of Oxidative Stress and Cholesterol Homeostasis in HepG2 Cells |
| title_short | Physicochemical Properties and Effects of Honeys on Key Biomarkers of Oxidative Stress and Cholesterol Homeostasis in HepG2 Cells |
| title_sort | physicochemical properties and effects of honeys on key biomarkers of oxidative stress and cholesterol homeostasis in hepg2 cells |
| topic | honey phytochemicals antioxidants cholesterol oxidative stress gene expression |
| url | https://www.mdpi.com/2072-6643/13/1/151 |
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