Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L.
Abstract Breeding rapeseed varieties with more main inflorescence siliques is an idea for developing rapeseed varieties that are suitable for light and simplified cultivation. The Brassica napus exhibited cluster bud of the main inflorescence (Bnclib) gene. At the fruiting stage, the main infloresce...
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| Format: | Article |
| Language: | English |
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BMC
2023-07-01
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| Series: | BMC Plant Biology |
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| Online Access: | https://doi.org/10.1186/s12870-023-04355-z |
| _version_ | 1797774299823603712 |
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| author | Wen Yin Zheng Zhe Yi Zhu Abdul Sami Meng Yuan Sun Yong Li Jian Hu Xing Zhi Qian Jin Xu Ma Mei Qi Wang Yan Yu Fu Gui Zhang Ke Jin Zhou Zong He Zhu |
| author_facet | Wen Yin Zheng Zhe Yi Zhu Abdul Sami Meng Yuan Sun Yong Li Jian Hu Xing Zhi Qian Jin Xu Ma Mei Qi Wang Yan Yu Fu Gui Zhang Ke Jin Zhou Zong He Zhu |
| author_sort | Wen Yin Zheng |
| collection | DOAJ |
| description | Abstract Breeding rapeseed varieties with more main inflorescence siliques is an idea for developing rapeseed varieties that are suitable for light and simplified cultivation. The Brassica napus exhibited cluster bud of the main inflorescence (Bnclib) gene. At the fruiting stage, the main inflorescence had more siliques, higher density, and more main inflorescences. Moreover, the top of the main inflorescence bifurcated. Genetic analysis showed that the separation ratio between Bnclib and the wild type in the F2 generation was 3:1, which indicated that the trait was a single-gene-dominant inheritance. Among the 24 candidate genes, only one gene, BnaA03g53930D, showed differential expression between the groups (False discovery rate, FDR ≤ 0.05, |log2FC|≤ 1). qPCR verification of the BnaA03g53930D gene between Huyou 17 and its Bnclib near-isogenic line showed that BnaA03g53930D was significantly differentially expressed in the stem tissue of Huyou 17 and its Bnclib near-isogenic line (Bnclib NIL). The determination of gibberellin (GA), brassinolide (BR), cytokinin (CTK), jasmonic acid (JA), growth hormone (IAA), and strigolactone (SL) content in the shoot apex of Huyou 17 by Bnclib NIL and wild type showed that all six hormones significantly differed between the Bnclib NIL and Huyou 17. It is necessary to conduct further research on the interactions between JA and the other five hormones and the main inflorescence bud clustering in B. napus. |
| first_indexed | 2024-03-12T22:19:01Z |
| format | Article |
| id | doaj.art-a26b09145a2c4f429ab8468de43bf85a |
| institution | Directory Open Access Journal |
| issn | 1471-2229 |
| language | English |
| last_indexed | 2024-03-12T22:19:01Z |
| publishDate | 2023-07-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Plant Biology |
| spelling | doaj.art-a26b09145a2c4f429ab8468de43bf85a2023-07-23T11:10:33ZengBMCBMC Plant Biology1471-22292023-07-0123111510.1186/s12870-023-04355-zMapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L.Wen Yin Zheng0Zhe Yi Zhu1Abdul Sami2Meng Yuan Sun3Yong Li4Jian Hu5Xing Zhi Qian6Jin Xu Ma7Mei Qi Wang8Yan Yu9Fu Gui Zhang10Ke Jin Zhou11Zong He Zhu12College of Agronomy, Anhui Agricultural UniversityCollege of Agronomy, Anhui Agricultural UniversityCollege of Agronomy, Anhui Agricultural UniversityCollege of Agronomy, Anhui Agricultural UniversityCollege of Agronomy, Anhui Agricultural UniversityCollege of Agronomy, Anhui Agricultural UniversityCollege of Agronomy, Anhui Agricultural UniversityCollege of Agronomy, Anhui Agricultural UniversityCollege of Agronomy, Anhui Agricultural UniversityCollege of Agronomy, Anhui Agricultural UniversityCollege of Agronomy, Anhui Agricultural UniversityCollege of Agronomy, Anhui Agricultural UniversityCollege of Agronomy, Anhui Agricultural UniversityAbstract Breeding rapeseed varieties with more main inflorescence siliques is an idea for developing rapeseed varieties that are suitable for light and simplified cultivation. The Brassica napus exhibited cluster bud of the main inflorescence (Bnclib) gene. At the fruiting stage, the main inflorescence had more siliques, higher density, and more main inflorescences. Moreover, the top of the main inflorescence bifurcated. Genetic analysis showed that the separation ratio between Bnclib and the wild type in the F2 generation was 3:1, which indicated that the trait was a single-gene-dominant inheritance. Among the 24 candidate genes, only one gene, BnaA03g53930D, showed differential expression between the groups (False discovery rate, FDR ≤ 0.05, |log2FC|≤ 1). qPCR verification of the BnaA03g53930D gene between Huyou 17 and its Bnclib near-isogenic line showed that BnaA03g53930D was significantly differentially expressed in the stem tissue of Huyou 17 and its Bnclib near-isogenic line (Bnclib NIL). The determination of gibberellin (GA), brassinolide (BR), cytokinin (CTK), jasmonic acid (JA), growth hormone (IAA), and strigolactone (SL) content in the shoot apex of Huyou 17 by Bnclib NIL and wild type showed that all six hormones significantly differed between the Bnclib NIL and Huyou 17. It is necessary to conduct further research on the interactions between JA and the other five hormones and the main inflorescence bud clustering in B. napus.https://doi.org/10.1186/s12870-023-04355-zBrassica napusPopulation segregation analysisFlower bud clustersEndogenous hormonesTranscriptome analysis |
| spellingShingle | Wen Yin Zheng Zhe Yi Zhu Abdul Sami Meng Yuan Sun Yong Li Jian Hu Xing Zhi Qian Jin Xu Ma Mei Qi Wang Yan Yu Fu Gui Zhang Ke Jin Zhou Zong He Zhu Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. BMC Plant Biology Brassica napus Population segregation analysis Flower bud clusters Endogenous hormones Transcriptome analysis |
| title | Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. |
| title_full | Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. |
| title_fullStr | Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. |
| title_full_unstemmed | Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. |
| title_short | Mapping and candidate gene analysis of clustered bud on the main inflorescence in Brassica napus L. |
| title_sort | mapping and candidate gene analysis of clustered bud on the main inflorescence in brassica napus l |
| topic | Brassica napus Population segregation analysis Flower bud clusters Endogenous hormones Transcriptome analysis |
| url | https://doi.org/10.1186/s12870-023-04355-z |
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