LncRNA CTBP1-AS2 sponges miR-216a to upregulate PTEN and suppress endometrial cancer cell invasion and migration
Abstract Background Although lncRNA CTBP1-AS2 has been functionally analyzed only in cardiomyocyte hypertrophy and diabetes, analysis of TCGA dataset revealed its downregulation in endometrial carcinoma (EC), indicating its involvement in EC. Results In this study we found that CTBP1-AS2 was downreg...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-04-01
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| Series: | Journal of Ovarian Research |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13048-020-00639-2 |
| _version_ | 1797965777132847104 |
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| author | Qing-an-zi Wang Yongxiu Yang Xiaolei Liang |
| author_facet | Qing-an-zi Wang Yongxiu Yang Xiaolei Liang |
| author_sort | Qing-an-zi Wang |
| collection | DOAJ |
| description | Abstract Background Although lncRNA CTBP1-AS2 has been functionally analyzed only in cardiomyocyte hypertrophy and diabetes, analysis of TCGA dataset revealed its downregulation in endometrial carcinoma (EC), indicating its involvement in EC. Results In this study we found that CTBP1-AS2 was downregulated in EC and correlated with poor survival. MiR-216a might form base pairs with CTBP1-AS2 based on RNA-RNA interaction, which was confirmed by luciferase activity assay. Interestingly, upregulation of PTEN was observed after CTBP1-AS2 overexpression. Transwell assay showed that CTBP1-AS2 and PTEN overexpression led to decreased cancer cell invasion and migration and reduced enhancing effects of miR-216a on cell invasion and migration. It was known that miR-216a targeted PTEN. Conclusion Therefore, CTBP1-AS2 may sponge miR-216a to upregulate PTEN, thereby suppressing endometrial cancer cell invasion and migration. |
| first_indexed | 2024-04-11T02:04:35Z |
| format | Article |
| id | doaj.art-a26d76897d8f442d8ff1014217c846d8 |
| institution | Directory Open Access Journal |
| issn | 1757-2215 |
| language | English |
| last_indexed | 2024-04-11T02:04:35Z |
| publishDate | 2020-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Journal of Ovarian Research |
| spelling | doaj.art-a26d76897d8f442d8ff1014217c846d82023-01-03T03:27:13ZengBMCJournal of Ovarian Research1757-22152020-04-011311610.1186/s13048-020-00639-2LncRNA CTBP1-AS2 sponges miR-216a to upregulate PTEN and suppress endometrial cancer cell invasion and migrationQing-an-zi Wang0Yongxiu Yang1Xiaolei Liang2Department of Obstetrics and Gynecology, Key Laboratory for Gynecologic Oncology Gansu Province, the First Hospital of Lanzhou UniversityDepartment of Obstetrics and Gynecology, Key Laboratory for Gynecologic Oncology Gansu Province, the First Hospital of Lanzhou UniversityDepartment of Obstetrics and Gynecology, Key Laboratory for Gynecologic Oncology Gansu Province, the First Hospital of Lanzhou UniversityAbstract Background Although lncRNA CTBP1-AS2 has been functionally analyzed only in cardiomyocyte hypertrophy and diabetes, analysis of TCGA dataset revealed its downregulation in endometrial carcinoma (EC), indicating its involvement in EC. Results In this study we found that CTBP1-AS2 was downregulated in EC and correlated with poor survival. MiR-216a might form base pairs with CTBP1-AS2 based on RNA-RNA interaction, which was confirmed by luciferase activity assay. Interestingly, upregulation of PTEN was observed after CTBP1-AS2 overexpression. Transwell assay showed that CTBP1-AS2 and PTEN overexpression led to decreased cancer cell invasion and migration and reduced enhancing effects of miR-216a on cell invasion and migration. It was known that miR-216a targeted PTEN. Conclusion Therefore, CTBP1-AS2 may sponge miR-216a to upregulate PTEN, thereby suppressing endometrial cancer cell invasion and migration.http://link.springer.com/article/10.1186/s13048-020-00639-2CTBP1-AS2Endometrial carcinomaSurvivalmiR-216aPTEN |
| spellingShingle | Qing-an-zi Wang Yongxiu Yang Xiaolei Liang LncRNA CTBP1-AS2 sponges miR-216a to upregulate PTEN and suppress endometrial cancer cell invasion and migration Journal of Ovarian Research CTBP1-AS2 Endometrial carcinoma Survival miR-216a PTEN |
| title | LncRNA CTBP1-AS2 sponges miR-216a to upregulate PTEN and suppress endometrial cancer cell invasion and migration |
| title_full | LncRNA CTBP1-AS2 sponges miR-216a to upregulate PTEN and suppress endometrial cancer cell invasion and migration |
| title_fullStr | LncRNA CTBP1-AS2 sponges miR-216a to upregulate PTEN and suppress endometrial cancer cell invasion and migration |
| title_full_unstemmed | LncRNA CTBP1-AS2 sponges miR-216a to upregulate PTEN and suppress endometrial cancer cell invasion and migration |
| title_short | LncRNA CTBP1-AS2 sponges miR-216a to upregulate PTEN and suppress endometrial cancer cell invasion and migration |
| title_sort | lncrna ctbp1 as2 sponges mir 216a to upregulate pten and suppress endometrial cancer cell invasion and migration |
| topic | CTBP1-AS2 Endometrial carcinoma Survival miR-216a PTEN |
| url | http://link.springer.com/article/10.1186/s13048-020-00639-2 |
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