Glucocerebroside reduces endothelial progenitor cell-induced angiogenesis
The recruitment of bone marrow-derived endothelial progenitor cells (EPCs) facilitates physiological and pathological processes involved in new blood vessel synthesis. Glucocerebroside, an extract of Cordyceps militaris, inhibits inflammatory cytokine production and monocyte migration, although its...
| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2019.1660623 |
| _version_ | 1818359433722331136 |
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| author | Hsiang-Ping Lee Shih-Wei Wang Yang-Chang Wu Chang-Hai Tsai Fuu-Jen Tsai Jing-Gung Chung Chih-Yang Huang Jai-Sing Yang Yuan-Man Hsu Mei-Chin Yin Te-Mao Li Chih-Hsin Tang |
| author_facet | Hsiang-Ping Lee Shih-Wei Wang Yang-Chang Wu Chang-Hai Tsai Fuu-Jen Tsai Jing-Gung Chung Chih-Yang Huang Jai-Sing Yang Yuan-Man Hsu Mei-Chin Yin Te-Mao Li Chih-Hsin Tang |
| author_sort | Hsiang-Ping Lee |
| collection | DOAJ |
| description | The recruitment of bone marrow-derived endothelial progenitor cells (EPCs) facilitates physiological and pathological processes involved in new blood vessel synthesis. Glucocerebroside, an extract of Cordyceps militaris, inhibits inflammatory cytokine production and monocyte migration, although its anti-angiogenic properties in human EPCs has remained largely unknown up until now. We describe how glucocerebroside reduces migration as well as tube formation induced by vascular endothelial growth factor (VEGF) stimulation in human EPCs, without affecting cell viability. This inhibitory effect was achieved through the focal adhesion kinase (FAK)/c-Src pathways. We also found that glucocerebroside reduced VEGF-promoted upregulation of the transcription factor Runx2 in the EPCs. The in vivo chick embryo chorioallantoic membrane model demonstrated that glucocerebroside reduces new vessel formation. Our investigation is the first to show that glucocerebroside reduces angiogenesis in human EPCs and to describe the underlying mechanisms. Further investigations are needed to examine the effects of glucocerebroside in other angiogenesis-related disorders. |
| first_indexed | 2024-12-13T20:44:49Z |
| format | Article |
| id | doaj.art-a276623225c34994b03ce6e77c2596ce |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-13T20:44:49Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-a276623225c34994b03ce6e77c2596ce2022-12-21T23:32:03ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432019-01-013011033104510.1080/09540105.2019.16606231660623Glucocerebroside reduces endothelial progenitor cell-induced angiogenesisHsiang-Ping Lee0Shih-Wei Wang1Yang-Chang Wu2Chang-Hai Tsai3Fuu-Jen Tsai4Jing-Gung Chung5Chih-Yang Huang6Jai-Sing Yang7Yuan-Man Hsu8Mei-Chin Yin9Te-Mao Li10Chih-Hsin Tang11China Medical UniversityMackay Medical CollegeKaohsiung Medical UniversityChina Medical University Children’s Hospital, China Medical UniversityChina Medical UniversityChina Medical UniversityAsia UniversityChina Medical University Hospital, China Medical UniversityChina Medical UniversityChina Medical University Hospital, China Medical UniversityChina Medical UniversityAsia UniversityThe recruitment of bone marrow-derived endothelial progenitor cells (EPCs) facilitates physiological and pathological processes involved in new blood vessel synthesis. Glucocerebroside, an extract of Cordyceps militaris, inhibits inflammatory cytokine production and monocyte migration, although its anti-angiogenic properties in human EPCs has remained largely unknown up until now. We describe how glucocerebroside reduces migration as well as tube formation induced by vascular endothelial growth factor (VEGF) stimulation in human EPCs, without affecting cell viability. This inhibitory effect was achieved through the focal adhesion kinase (FAK)/c-Src pathways. We also found that glucocerebroside reduced VEGF-promoted upregulation of the transcription factor Runx2 in the EPCs. The in vivo chick embryo chorioallantoic membrane model demonstrated that glucocerebroside reduces new vessel formation. Our investigation is the first to show that glucocerebroside reduces angiogenesis in human EPCs and to describe the underlying mechanisms. Further investigations are needed to examine the effects of glucocerebroside in other angiogenesis-related disorders.http://dx.doi.org/10.1080/09540105.2019.1660623endothelial progenitor cellsangiogenesisglucocerebrosidevegf-a |
| spellingShingle | Hsiang-Ping Lee Shih-Wei Wang Yang-Chang Wu Chang-Hai Tsai Fuu-Jen Tsai Jing-Gung Chung Chih-Yang Huang Jai-Sing Yang Yuan-Man Hsu Mei-Chin Yin Te-Mao Li Chih-Hsin Tang Glucocerebroside reduces endothelial progenitor cell-induced angiogenesis Food and Agricultural Immunology endothelial progenitor cells angiogenesis glucocerebroside vegf-a |
| title | Glucocerebroside reduces endothelial progenitor cell-induced angiogenesis |
| title_full | Glucocerebroside reduces endothelial progenitor cell-induced angiogenesis |
| title_fullStr | Glucocerebroside reduces endothelial progenitor cell-induced angiogenesis |
| title_full_unstemmed | Glucocerebroside reduces endothelial progenitor cell-induced angiogenesis |
| title_short | Glucocerebroside reduces endothelial progenitor cell-induced angiogenesis |
| title_sort | glucocerebroside reduces endothelial progenitor cell induced angiogenesis |
| topic | endothelial progenitor cells angiogenesis glucocerebroside vegf-a |
| url | http://dx.doi.org/10.1080/09540105.2019.1660623 |
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