Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes
The flow cytometry analysis of human peripheral blood monocytes separated by a two step density gradient centrifugation is reported. The expression of mRNA level of adenosine receptor (AdoR) subtypes (A1, A2A, A2B and A3) and interleukin 6 (IL-6), interleukin 8 (IL-8) and vascular endothelial growth...
| Main Authors: | , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Siberian State Medical University (Tomsk)
2011-06-01
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| Series: | Бюллетень сибирской медицины |
| Subjects: | |
| Online Access: | https://bulletin.ssmu.ru/jour/article/view/1392 |
| _version_ | 1797873052565897216 |
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| author | S. V. Ryzhov K. S. Yuryeva K. V. Goremykin Ye. V. Korotkaya I. V. Saltykova Yu. A. Yakovleva Ye. S. Kulikov O. S. Fyodorova Ye. E. Kremer N. S. Fattakhov A. E. Sazonov |
| author_facet | S. V. Ryzhov K. S. Yuryeva K. V. Goremykin Ye. V. Korotkaya I. V. Saltykova Yu. A. Yakovleva Ye. S. Kulikov O. S. Fyodorova Ye. E. Kremer N. S. Fattakhov A. E. Sazonov |
| author_sort | S. V. Ryzhov |
| collection | DOAJ |
| description | The flow cytometry analysis of human peripheral blood monocytes separated by a two step density gradient centrifugation is reported. The expression of mRNA level of adenosine receptor (AdoR) subtypes (A1, A2A, A2B and A3) and interleukin 6 (IL-6), interleukin 8 (IL-8) and vascular endothelial growth factor (VEGF) in monocytes were determined using real-time PCR. We found considerable variation across individuals in mRNA expression levels of paracrine factors after the stimulation of adenosine receptors. Our findings suggests the role of adenosinergic system accounted for interindividual differences in monocyte activation. |
| first_indexed | 2024-04-10T01:10:03Z |
| format | Article |
| id | doaj.art-a2772ef9bab5410da37349c1c2cb575d |
| institution | Directory Open Access Journal |
| issn | 1682-0363 1819-3684 |
| language | English |
| last_indexed | 2024-04-10T01:10:03Z |
| publishDate | 2011-06-01 |
| publisher | Siberian State Medical University (Tomsk) |
| record_format | Article |
| series | Бюллетень сибирской медицины |
| spelling | doaj.art-a2772ef9bab5410da37349c1c2cb575d2023-03-13T09:58:15ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842011-06-01103546110.20538/1682-0363-2011-3-54-61904Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytesS. V. Ryzhov0K. S. Yuryeva1K. V. Goremykin2Ye. V. Korotkaya3I. V. Saltykova4Yu. A. Yakovleva5Ye. S. Kulikov6O. S. Fyodorova7Ye. E. Kremer8N. S. Fattakhov9A. E. Sazonov10Университет ВандербилтаСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетСибирский государственный медицинский университетThe flow cytometry analysis of human peripheral blood monocytes separated by a two step density gradient centrifugation is reported. The expression of mRNA level of adenosine receptor (AdoR) subtypes (A1, A2A, A2B and A3) and interleukin 6 (IL-6), interleukin 8 (IL-8) and vascular endothelial growth factor (VEGF) in monocytes were determined using real-time PCR. We found considerable variation across individuals in mRNA expression levels of paracrine factors after the stimulation of adenosine receptors. Our findings suggests the role of adenosinergic system accounted for interindividual differences in monocyte activation.https://bulletin.ssmu.ru/jour/article/view/1392моноцитыаденозинаденозиновые рецепторыпаракринные факторыинтерлейкин-6интерлейкин-8фактор роста эндотелия сосудов |
| spellingShingle | S. V. Ryzhov K. S. Yuryeva K. V. Goremykin Ye. V. Korotkaya I. V. Saltykova Yu. A. Yakovleva Ye. S. Kulikov O. S. Fyodorova Ye. E. Kremer N. S. Fattakhov A. E. Sazonov Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes Бюллетень сибирской медицины моноциты аденозин аденозиновые рецепторы паракринные факторы интерлейкин-6 интерлейкин-8 фактор роста эндотелия сосудов |
| title | Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes |
| title_full | Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes |
| title_fullStr | Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes |
| title_full_unstemmed | Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes |
| title_short | Adenosine-dependent regulation of paracrine factors expression in human venous blood monocytes |
| title_sort | adenosine dependent regulation of paracrine factors expression in human venous blood monocytes |
| topic | моноциты аденозин аденозиновые рецепторы паракринные факторы интерлейкин-6 интерлейкин-8 фактор роста эндотелия сосудов |
| url | https://bulletin.ssmu.ru/jour/article/view/1392 |
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