Casticin protected against neuronal injury and inhibited the TLR4/NF‐κB pathway after middle cerebral artery occlusion in rats
Abstract Although stroke is a major human neurological disease, there is a paucity of effective neuroprotectants that can improve its treatment. Casticin is a natural monomer drug with many biological effects such as anti‐inflammatory and anti‐tumor actions. However, it is not clear whether it has a...
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| Format: | Article |
| Language: | English |
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Wiley
2021-04-01
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| Series: | Pharmacology Research & Perspectives |
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| Online Access: | https://doi.org/10.1002/prp2.752 |
| _version_ | 1818648731688370176 |
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| author | Dan Huang Jiafan Zhou Wenning Li Li Zhang Xiaomeng Wang Qiang Liu |
| author_facet | Dan Huang Jiafan Zhou Wenning Li Li Zhang Xiaomeng Wang Qiang Liu |
| author_sort | Dan Huang |
| collection | DOAJ |
| description | Abstract Although stroke is a major human neurological disease, there is a paucity of effective neuroprotectants that can improve its treatment. Casticin is a natural monomer drug with many biological effects such as anti‐inflammatory and anti‐tumor actions. However, it is not clear whether it has a neuroprotective effect in ischemic stroke. In this study, the neuroprotective effect of casticin in a rat middle cerebral artery occlusion (MCAO) model was investigated. Results showed that casticin reduced the volume of the cerebral infarction, mNSS scores, swimming distance, time to find the submerged platform, and serum concentrations of TNF‐α, TGF‐β, IL‐6 in MCAO rats. Moreover, casticin also decreased the expression of TLR4, NF‐κB p65, and NF‐κB p50 proteins and reversed the reduced expression of IκB protein in the brain tissue of MCAO rats. The in vitro study revealed that casticin decreased apoptosis of OGD/R‐PC12 cells, reduced the expression of TLR4, NF‐κB p65, and NF‐κB p50, while increased IκB protein expression. In conclusion, casticin improved the neurological functions of MCAO rats via inhibiting the TLR4/NF‐κB pathway and might have the potential to be developed into a neuroprotective agent for stroke patients. |
| first_indexed | 2024-12-17T01:23:05Z |
| format | Article |
| id | doaj.art-a2774c73fa094f88ab07d7ba613addde |
| institution | Directory Open Access Journal |
| issn | 2052-1707 |
| language | English |
| last_indexed | 2024-12-17T01:23:05Z |
| publishDate | 2021-04-01 |
| publisher | Wiley |
| record_format | Article |
| series | Pharmacology Research & Perspectives |
| spelling | doaj.art-a2774c73fa094f88ab07d7ba613addde2022-12-21T22:08:46ZengWileyPharmacology Research & Perspectives2052-17072021-04-0192n/an/a10.1002/prp2.752Casticin protected against neuronal injury and inhibited the TLR4/NF‐κB pathway after middle cerebral artery occlusion in ratsDan Huang0Jiafan Zhou1Wenning Li2Li Zhang3Xiaomeng Wang4Qiang Liu5Department of Neurology Hainan General HospitalHainan Affiliated Hospital of Hainan Medical University Haikou ChinaDepartment of Neurology Qionghai People's Hospital Qionghai ChinaDepartment of Neurology Hainan General HospitalHainan Affiliated Hospital of Hainan Medical University Haikou ChinaDepartment of Pharmacology Hainan Medical University Haikou ChinaDepartment of Pharmacology Hainan Medical University Haikou ChinaDepartment of Pharmacology Hainan Medical University Haikou ChinaAbstract Although stroke is a major human neurological disease, there is a paucity of effective neuroprotectants that can improve its treatment. Casticin is a natural monomer drug with many biological effects such as anti‐inflammatory and anti‐tumor actions. However, it is not clear whether it has a neuroprotective effect in ischemic stroke. In this study, the neuroprotective effect of casticin in a rat middle cerebral artery occlusion (MCAO) model was investigated. Results showed that casticin reduced the volume of the cerebral infarction, mNSS scores, swimming distance, time to find the submerged platform, and serum concentrations of TNF‐α, TGF‐β, IL‐6 in MCAO rats. Moreover, casticin also decreased the expression of TLR4, NF‐κB p65, and NF‐κB p50 proteins and reversed the reduced expression of IκB protein in the brain tissue of MCAO rats. The in vitro study revealed that casticin decreased apoptosis of OGD/R‐PC12 cells, reduced the expression of TLR4, NF‐κB p65, and NF‐κB p50, while increased IκB protein expression. In conclusion, casticin improved the neurological functions of MCAO rats via inhibiting the TLR4/NF‐κB pathway and might have the potential to be developed into a neuroprotective agent for stroke patients.https://doi.org/10.1002/prp2.752casticinneuroprotectivestrokeTLR4/NF‐κB pathway |
| spellingShingle | Dan Huang Jiafan Zhou Wenning Li Li Zhang Xiaomeng Wang Qiang Liu Casticin protected against neuronal injury and inhibited the TLR4/NF‐κB pathway after middle cerebral artery occlusion in rats Pharmacology Research & Perspectives casticin neuroprotective stroke TLR4/NF‐κB pathway |
| title | Casticin protected against neuronal injury and inhibited the TLR4/NF‐κB pathway after middle cerebral artery occlusion in rats |
| title_full | Casticin protected against neuronal injury and inhibited the TLR4/NF‐κB pathway after middle cerebral artery occlusion in rats |
| title_fullStr | Casticin protected against neuronal injury and inhibited the TLR4/NF‐κB pathway after middle cerebral artery occlusion in rats |
| title_full_unstemmed | Casticin protected against neuronal injury and inhibited the TLR4/NF‐κB pathway after middle cerebral artery occlusion in rats |
| title_short | Casticin protected against neuronal injury and inhibited the TLR4/NF‐κB pathway after middle cerebral artery occlusion in rats |
| title_sort | casticin protected against neuronal injury and inhibited the tlr4 nf κb pathway after middle cerebral artery occlusion in rats |
| topic | casticin neuroprotective stroke TLR4/NF‐κB pathway |
| url | https://doi.org/10.1002/prp2.752 |
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