Sensitive and selective electrochemical aptasensor via diazonium-coupling reaction for label-free determination of oxytetracycline in milk samples
As one kind of tetracycline antibiotics, oxytetracycline (OTC) residues in animal-derived foods can do serious harm to human health. Though aptasensors for the detection of OTC are widely reported, label-free electrochemical aptasensor with low cost, simple preparation and good applicability should...
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| Format: | Article |
| Language: | English |
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Elsevier
2020-11-01
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| Series: | Sensors and Actuators Reports |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666053920300060 |
| _version_ | 1818414229321940992 |
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| author | Yukun Yang Wenyan Yan Yuanyuan Guo Xiaomin Wang Fuyuan Zhang Ligang Yu Caixia Guo Guozhen Fang |
| author_facet | Yukun Yang Wenyan Yan Yuanyuan Guo Xiaomin Wang Fuyuan Zhang Ligang Yu Caixia Guo Guozhen Fang |
| author_sort | Yukun Yang |
| collection | DOAJ |
| description | As one kind of tetracycline antibiotics, oxytetracycline (OTC) residues in animal-derived foods can do serious harm to human health. Though aptasensors for the detection of OTC are widely reported, label-free electrochemical aptasensor with low cost, simple preparation and good applicability should be further explored to meet the current urgent needs. In this study, a label-free electrochemical aptasensor for OTC determination was designed. The detection was based on specific recognition by the aptamer covalently-bound on the sensing interface via diazonium coupling reaction, which was verified by computer simulation. Experiment results showed that the differential pulse voltammetry (DPV) current response of the constructed electrochemical aptasensor was linear with the logarithm of OTC concentration in the range of 1.0 × 10−9 g mL−1–1.0 × 10−4 g mL−1 (R2 = 0.9897) with the limit of detection of 2.29 × 10−10 g mL−1. The proposed aptasensor indicated good selectivity, reproducibility and stability, which could be attributed to the stable modification of aptamer on the sensing interface. The OTC in the milk sample was detected by the spiked recovery method, and good results were obtained, with the recovery rate being 87.0%-99.3%. The proposed label-free electrochemical aptasensor can provide methods for low-cost, accurate and rapid detection of antibiotic residues. |
| first_indexed | 2024-12-14T11:15:47Z |
| format | Article |
| id | doaj.art-a277b1d3112d433bae2e05e716181513 |
| institution | Directory Open Access Journal |
| issn | 2666-0539 |
| language | English |
| last_indexed | 2024-12-14T11:15:47Z |
| publishDate | 2020-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Sensors and Actuators Reports |
| spelling | doaj.art-a277b1d3112d433bae2e05e7161815132022-12-21T23:04:03ZengElsevierSensors and Actuators Reports2666-05392020-11-0121100009Sensitive and selective electrochemical aptasensor via diazonium-coupling reaction for label-free determination of oxytetracycline in milk samplesYukun Yang0Wenyan Yan1Yuanyuan Guo2Xiaomin Wang3Fuyuan Zhang4Ligang Yu5Caixia Guo6Guozhen Fang7School of Life Science, Shanxi University, Taiyuan 030006, China; Corresponding authors.School of Life Science, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaCollege of Pharmaceutical and Food Engineering, Shanxi University of Chinese Medicine, Jinzhong 030619, China; Corresponding authors.College of Food Science and Technology, Hebei Agricultural University, Baoding 071001, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaSchool of Life Science, Shanxi University, Taiyuan 030006, ChinaState Key Laboratory of Food Nutrition and Safety, Tianjin University of Science and Technology, Tianjin 300457, China; Corresponding authors.As one kind of tetracycline antibiotics, oxytetracycline (OTC) residues in animal-derived foods can do serious harm to human health. Though aptasensors for the detection of OTC are widely reported, label-free electrochemical aptasensor with low cost, simple preparation and good applicability should be further explored to meet the current urgent needs. In this study, a label-free electrochemical aptasensor for OTC determination was designed. The detection was based on specific recognition by the aptamer covalently-bound on the sensing interface via diazonium coupling reaction, which was verified by computer simulation. Experiment results showed that the differential pulse voltammetry (DPV) current response of the constructed electrochemical aptasensor was linear with the logarithm of OTC concentration in the range of 1.0 × 10−9 g mL−1–1.0 × 10−4 g mL−1 (R2 = 0.9897) with the limit of detection of 2.29 × 10−10 g mL−1. The proposed aptasensor indicated good selectivity, reproducibility and stability, which could be attributed to the stable modification of aptamer on the sensing interface. The OTC in the milk sample was detected by the spiked recovery method, and good results were obtained, with the recovery rate being 87.0%-99.3%. The proposed label-free electrochemical aptasensor can provide methods for low-cost, accurate and rapid detection of antibiotic residues.http://www.sciencedirect.com/science/article/pii/S2666053920300060Electrochemical aptasensorLabel-free detectionCovalent bondDiazonium-coupling reactionOxytetracycline |
| spellingShingle | Yukun Yang Wenyan Yan Yuanyuan Guo Xiaomin Wang Fuyuan Zhang Ligang Yu Caixia Guo Guozhen Fang Sensitive and selective electrochemical aptasensor via diazonium-coupling reaction for label-free determination of oxytetracycline in milk samples Sensors and Actuators Reports Electrochemical aptasensor Label-free detection Covalent bond Diazonium-coupling reaction Oxytetracycline |
| title | Sensitive and selective electrochemical aptasensor via diazonium-coupling reaction for label-free determination of oxytetracycline in milk samples |
| title_full | Sensitive and selective electrochemical aptasensor via diazonium-coupling reaction for label-free determination of oxytetracycline in milk samples |
| title_fullStr | Sensitive and selective electrochemical aptasensor via diazonium-coupling reaction for label-free determination of oxytetracycline in milk samples |
| title_full_unstemmed | Sensitive and selective electrochemical aptasensor via diazonium-coupling reaction for label-free determination of oxytetracycline in milk samples |
| title_short | Sensitive and selective electrochemical aptasensor via diazonium-coupling reaction for label-free determination of oxytetracycline in milk samples |
| title_sort | sensitive and selective electrochemical aptasensor via diazonium coupling reaction for label free determination of oxytetracycline in milk samples |
| topic | Electrochemical aptasensor Label-free detection Covalent bond Diazonium-coupling reaction Oxytetracycline |
| url | http://www.sciencedirect.com/science/article/pii/S2666053920300060 |
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