Rice Premature Leaf Senescence 2, Encoding a Glycosyltransferase (GT), Is Involved in Leaf Senescence
Premature leaf senescence (PLS), which has a significant impact on yield, is caused by various underlying mechanisms. Glycosyltransferases, which function in glycosyl transfer from activated nucleotides to aglycones, are involved in diverse biological processes, but their roles in rice leaf senescen...
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| Format: | Article |
| Language: | English |
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Frontiers Media S.A.
2018-04-01
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| Series: | Frontiers in Plant Science |
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| Online Access: | http://journal.frontiersin.org/article/10.3389/fpls.2018.00560/full |
| _version_ | 1818500256741982208 |
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| author | Min Wang Tao Zhang Hao Peng Sheng Luo Juejie Tan Kaifeng Jiang Yueqin Heng Xin Zhang Xiuping Guo Jiakui Zheng Zhijun Cheng |
| author_facet | Min Wang Tao Zhang Hao Peng Sheng Luo Juejie Tan Kaifeng Jiang Yueqin Heng Xin Zhang Xiuping Guo Jiakui Zheng Zhijun Cheng |
| author_sort | Min Wang |
| collection | DOAJ |
| description | Premature leaf senescence (PLS), which has a significant impact on yield, is caused by various underlying mechanisms. Glycosyltransferases, which function in glycosyl transfer from activated nucleotides to aglycones, are involved in diverse biological processes, but their roles in rice leaf senescence remain elusive. Here, we isolated and characterized a leaf senescence-related gene from the Premature Leaf Senescent mutant (pls2). The mutant phenotype began with leaf yellowing at tillering and resulted in PLS during the reproductive stage. Leaf senescence was associated with an increase in hydrogen peroxide (H2O2) content accompanied with pronounced decreases in net photosynthetic rate, stomatal conductance, and transpiration rate. Map-based cloning revealed that a mutation in LOC_Os03g15840 (PLS2), a putative glycosyltransferase- encoding gene, was responsible for the defective phenotype. PLS2 expression was detected in all tissues surveyed, but predominantly in leaf mesophyll cells. Subcellular localization of the PLS2 was in the endoplasmic reticulum. The pls2 mutant accumulated higher levels of sucrose together with decreased expression of sucrose metabolizing genes compared with wild type. These data suggested that the PLS2 allele is essential for normal leaf senescence and its mutation resulted in PLS. |
| first_indexed | 2024-12-10T20:40:17Z |
| format | Article |
| id | doaj.art-a2788a5a7a3540c4af309a84d3fc4525 |
| institution | Directory Open Access Journal |
| issn | 1664-462X |
| language | English |
| last_indexed | 2024-12-10T20:40:17Z |
| publishDate | 2018-04-01 |
| publisher | Frontiers Media S.A. |
| record_format | Article |
| series | Frontiers in Plant Science |
| spelling | doaj.art-a2788a5a7a3540c4af309a84d3fc45252022-12-22T01:34:24ZengFrontiers Media S.A.Frontiers in Plant Science1664-462X2018-04-01910.3389/fpls.2018.00560338392Rice Premature Leaf Senescence 2, Encoding a Glycosyltransferase (GT), Is Involved in Leaf SenescenceMin Wang0Tao Zhang1Hao Peng2Sheng Luo3Juejie Tan4Kaifeng Jiang5Yueqin Heng6Xin Zhang7Xiuping Guo8Jiakui Zheng9Zhijun Cheng10National Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Rice and Sorghum, Sichuan Academy of Agricultural Sciences, Deyang, ChinaDepartment of Life Science and Engineering, Jining University, Jining, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Rice and Sorghum, Sichuan Academy of Agricultural Sciences, Deyang, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaInstitute of Rice and Sorghum, Sichuan Academy of Agricultural Sciences, Deyang, ChinaNational Key Facility for Crop Gene Resources and Genetic Improvement, Institute of Crop Science, Chinese Academy of Agricultural Sciences, Beijing, ChinaPremature leaf senescence (PLS), which has a significant impact on yield, is caused by various underlying mechanisms. Glycosyltransferases, which function in glycosyl transfer from activated nucleotides to aglycones, are involved in diverse biological processes, but their roles in rice leaf senescence remain elusive. Here, we isolated and characterized a leaf senescence-related gene from the Premature Leaf Senescent mutant (pls2). The mutant phenotype began with leaf yellowing at tillering and resulted in PLS during the reproductive stage. Leaf senescence was associated with an increase in hydrogen peroxide (H2O2) content accompanied with pronounced decreases in net photosynthetic rate, stomatal conductance, and transpiration rate. Map-based cloning revealed that a mutation in LOC_Os03g15840 (PLS2), a putative glycosyltransferase- encoding gene, was responsible for the defective phenotype. PLS2 expression was detected in all tissues surveyed, but predominantly in leaf mesophyll cells. Subcellular localization of the PLS2 was in the endoplasmic reticulum. The pls2 mutant accumulated higher levels of sucrose together with decreased expression of sucrose metabolizing genes compared with wild type. These data suggested that the PLS2 allele is essential for normal leaf senescence and its mutation resulted in PLS.http://journal.frontiersin.org/article/10.3389/fpls.2018.00560/fullglycosyltransferaseGTleaf senescenceOryza sativaPLS2sucrose |
| spellingShingle | Min Wang Tao Zhang Hao Peng Sheng Luo Juejie Tan Kaifeng Jiang Yueqin Heng Xin Zhang Xiuping Guo Jiakui Zheng Zhijun Cheng Rice Premature Leaf Senescence 2, Encoding a Glycosyltransferase (GT), Is Involved in Leaf Senescence Frontiers in Plant Science glycosyltransferase GT leaf senescence Oryza sativa PLS2 sucrose |
| title | Rice Premature Leaf Senescence 2, Encoding a Glycosyltransferase (GT), Is Involved in Leaf Senescence |
| title_full | Rice Premature Leaf Senescence 2, Encoding a Glycosyltransferase (GT), Is Involved in Leaf Senescence |
| title_fullStr | Rice Premature Leaf Senescence 2, Encoding a Glycosyltransferase (GT), Is Involved in Leaf Senescence |
| title_full_unstemmed | Rice Premature Leaf Senescence 2, Encoding a Glycosyltransferase (GT), Is Involved in Leaf Senescence |
| title_short | Rice Premature Leaf Senescence 2, Encoding a Glycosyltransferase (GT), Is Involved in Leaf Senescence |
| title_sort | rice premature leaf senescence 2 encoding a glycosyltransferase gt is involved in leaf senescence |
| topic | glycosyltransferase GT leaf senescence Oryza sativa PLS2 sucrose |
| url | http://journal.frontiersin.org/article/10.3389/fpls.2018.00560/full |
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