Determination of Selenium in Selenium—Enriched Products by Specific Ratiometric Fluorescence
Selenium (Se), as one of the essential and nutrient components of living organisms and plants, plays an important role in life activities, while excessive selenium is hazardous to human health. So, the establishment of an effective method for simple, rapid, and highly sensitive determination of sele...
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MDPI AG
2023-11-01
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| Series: | Sensors |
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| Online Access: | https://www.mdpi.com/1424-8220/23/22/9187 |
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| author | Munire Aimaitiniyazi Turghun Muhammad Ayzukram Yasen Sainawaer Abula Almire Dolkun Zulhumar Tursun |
| author_facet | Munire Aimaitiniyazi Turghun Muhammad Ayzukram Yasen Sainawaer Abula Almire Dolkun Zulhumar Tursun |
| author_sort | Munire Aimaitiniyazi |
| collection | DOAJ |
| description | Selenium (Se), as one of the essential and nutrient components of living organisms and plants, plays an important role in life activities, while excessive selenium is hazardous to human health. So, the establishment of an effective method for simple, rapid, and highly sensitive determination of selenium content is crucial in the field of food composition analysis and other areas. In this paper, a novel and simple ratiometric fluorescence method for the determination of Se has been developed using 9-anthracenemethanol (AM) as the ratiometric fluorescence reagent on the basis of the conventional fluorometric assay which utilized 2,3-diaminonapthalene (DAN) as fluorescent ligand. The ratiometric method was compared with the conventional method with respect to precision and accuracy. The inter-day and intra-day precisions (RSDs) of the ratiometric fluorescence method ranged from 2.08 to 2.78% and 1.28 to 1.84%, with mean recoveries of 93.2~98.0% and limit of detection (LOD) and limit of quantification (LOQ) of 0.0016 and 0.0049 μg/mL, respectively. This method was successfully applied to the determination of total selenium in selenium-enriched milk and selenium-supplemented shampoo, with the results in agreement with those obtained by inductively coupled plasma mass spectrometer (ICP-MS). The results demonstrated that the precision and accuracy of the ratiometric fluorescence method were superior to those of the conventional fluorescence method, and the interferences of various environmental factors were effectively eliminated. The precision and accuracy of the conventional method can be significantly improved by simply adding an elaborately selected ratiometric fluorescence reagent, and the new method will have broader practical applications. |
| first_indexed | 2024-03-09T16:28:38Z |
| format | Article |
| id | doaj.art-a27d7a733e134912b52ff159d46a11cf |
| institution | Directory Open Access Journal |
| issn | 1424-8220 |
| language | English |
| last_indexed | 2024-03-09T16:28:38Z |
| publishDate | 2023-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Sensors |
| spelling | doaj.art-a27d7a733e134912b52ff159d46a11cf2023-11-24T15:05:40ZengMDPI AGSensors1424-82202023-11-012322918710.3390/s23229187Determination of Selenium in Selenium—Enriched Products by Specific Ratiometric FluorescenceMunire Aimaitiniyazi0Turghun Muhammad1Ayzukram Yasen2Sainawaer Abula3Almire Dolkun4Zulhumar Tursun5State Key Laboratory of Chemistry and Utilization of Carbon-Based Energy Resources, College of Chemistry, Xinjiang University, Urumqi 830017, ChinaState Key Laboratory of Chemistry and Utilization of Carbon-Based Energy Resources, College of Chemistry, Xinjiang University, Urumqi 830017, ChinaKey Lab of Natural Product Chemistry and Application, School of Chemistry and Chemical Engineering, Yili Normal University, Yining 835000, ChinaSchool of Safety Science and Engineering, Xinjiang Engineering Institute, Urumqi 830023, ChinaState Key Laboratory of Chemistry and Utilization of Carbon-Based Energy Resources, College of Chemistry, Xinjiang University, Urumqi 830017, ChinaState Key Laboratory of Chemistry and Utilization of Carbon-Based Energy Resources, College of Chemistry, Xinjiang University, Urumqi 830017, ChinaSelenium (Se), as one of the essential and nutrient components of living organisms and plants, plays an important role in life activities, while excessive selenium is hazardous to human health. So, the establishment of an effective method for simple, rapid, and highly sensitive determination of selenium content is crucial in the field of food composition analysis and other areas. In this paper, a novel and simple ratiometric fluorescence method for the determination of Se has been developed using 9-anthracenemethanol (AM) as the ratiometric fluorescence reagent on the basis of the conventional fluorometric assay which utilized 2,3-diaminonapthalene (DAN) as fluorescent ligand. The ratiometric method was compared with the conventional method with respect to precision and accuracy. The inter-day and intra-day precisions (RSDs) of the ratiometric fluorescence method ranged from 2.08 to 2.78% and 1.28 to 1.84%, with mean recoveries of 93.2~98.0% and limit of detection (LOD) and limit of quantification (LOQ) of 0.0016 and 0.0049 μg/mL, respectively. This method was successfully applied to the determination of total selenium in selenium-enriched milk and selenium-supplemented shampoo, with the results in agreement with those obtained by inductively coupled plasma mass spectrometer (ICP-MS). The results demonstrated that the precision and accuracy of the ratiometric fluorescence method were superior to those of the conventional fluorescence method, and the interferences of various environmental factors were effectively eliminated. The precision and accuracy of the conventional method can be significantly improved by simply adding an elaborately selected ratiometric fluorescence reagent, and the new method will have broader practical applications.https://www.mdpi.com/1424-8220/23/22/9187ratiometric fluorescenceseleniumdeterminationinternal referencefood safety |
| spellingShingle | Munire Aimaitiniyazi Turghun Muhammad Ayzukram Yasen Sainawaer Abula Almire Dolkun Zulhumar Tursun Determination of Selenium in Selenium—Enriched Products by Specific Ratiometric Fluorescence Sensors ratiometric fluorescence selenium determination internal reference food safety |
| title | Determination of Selenium in Selenium—Enriched Products by Specific Ratiometric Fluorescence |
| title_full | Determination of Selenium in Selenium—Enriched Products by Specific Ratiometric Fluorescence |
| title_fullStr | Determination of Selenium in Selenium—Enriched Products by Specific Ratiometric Fluorescence |
| title_full_unstemmed | Determination of Selenium in Selenium—Enriched Products by Specific Ratiometric Fluorescence |
| title_short | Determination of Selenium in Selenium—Enriched Products by Specific Ratiometric Fluorescence |
| title_sort | determination of selenium in selenium enriched products by specific ratiometric fluorescence |
| topic | ratiometric fluorescence selenium determination internal reference food safety |
| url | https://www.mdpi.com/1424-8220/23/22/9187 |
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