A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion
Cancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chi...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-10-01
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| Series: | Cells |
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| Online Access: | https://www.mdpi.com/2073-4409/10/11/2855 |
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| author | Gaurav Pendharkar Yen-Ta Lu Chia-Ming Chang Meng-Ping Lu Chung-Huan Lu Chih-Chen Chen Cheng-Hsien Liu |
| author_facet | Gaurav Pendharkar Yen-Ta Lu Chia-Ming Chang Meng-Ping Lu Chung-Huan Lu Chih-Chen Chen Cheng-Hsien Liu |
| author_sort | Gaurav Pendharkar |
| collection | DOAJ |
| description | Cancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chip (MFC) consisting of a thin through-hole membrane sandwiched between a mirrored array of microfluidic channels and saw-tooth shaped titanium electrodes on the glass. We discuss the design and operation of MFC and show its applicability for cell fusion. The proposed device combines passive hydrodynamic phenomenon and gravitational sedimentation, which allows the transportation and trapping of homotypic and heterotypic cells in large numbers with pairing efficiencies of 75~78% and fusion efficiencies of 73%. Additionally, we also report properties of fused cells from cell biology perspectives, including combined fluorescence-labeled intracellular materials from THP1 and A549, mixed cell morphology, and cell viability. The MFC can be tuned for pairing and fusion of cells with a similar protocol for different cell types. The MFC can be easily disconnected from the test setup for further analysis. |
| first_indexed | 2024-03-10T05:37:36Z |
| format | Article |
| id | doaj.art-a28030de020f474ebe8c3e652fa35f1a |
| institution | Directory Open Access Journal |
| issn | 2073-4409 |
| language | English |
| last_indexed | 2024-03-10T05:37:36Z |
| publishDate | 2021-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Cells |
| spelling | doaj.art-a28030de020f474ebe8c3e652fa35f1a2023-11-22T22:47:44ZengMDPI AGCells2073-44092021-10-011011285510.3390/cells10112855A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and FusionGaurav Pendharkar0Yen-Ta Lu1Chia-Ming Chang2Meng-Ping Lu3Chung-Huan Lu4Chih-Chen Chen5Cheng-Hsien Liu6Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30044, TaiwanChest Department, MacKay Memorial Hospital, New Taipei City 10449, TaiwanDepartment of Medical Research, MacKay Memorial Hospital, New Taipei City 10449, TaiwanDepartment of Medical Research, MacKay Memorial Hospital, New Taipei City 10449, TaiwanDepartment of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30044, TaiwanDepartment of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30044, TaiwanDepartment of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30044, TaiwanCancer cell–immune cell hybrids and cancer immunotherapy have attracted much attention in recent years. The design of efficient cell pairing and fusion chips for hybridoma generation has been, subsequently, a subject of great interest. Here, we report a three-layered integrated Microfluidic Flip-Chip (MFC) consisting of a thin through-hole membrane sandwiched between a mirrored array of microfluidic channels and saw-tooth shaped titanium electrodes on the glass. We discuss the design and operation of MFC and show its applicability for cell fusion. The proposed device combines passive hydrodynamic phenomenon and gravitational sedimentation, which allows the transportation and trapping of homotypic and heterotypic cells in large numbers with pairing efficiencies of 75~78% and fusion efficiencies of 73%. Additionally, we also report properties of fused cells from cell biology perspectives, including combined fluorescence-labeled intracellular materials from THP1 and A549, mixed cell morphology, and cell viability. The MFC can be tuned for pairing and fusion of cells with a similar protocol for different cell types. The MFC can be easily disconnected from the test setup for further analysis.https://www.mdpi.com/2073-4409/10/11/2855hydrodynamic trappingcell pairingdielectrophoresiscell fusion |
| spellingShingle | Gaurav Pendharkar Yen-Ta Lu Chia-Ming Chang Meng-Ping Lu Chung-Huan Lu Chih-Chen Chen Cheng-Hsien Liu A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion Cells hydrodynamic trapping cell pairing dielectrophoresis cell fusion |
| title | A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion |
| title_full | A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion |
| title_fullStr | A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion |
| title_full_unstemmed | A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion |
| title_short | A Microfluidic Flip-Chip Combining Hydrodynamic Trapping and Gravitational Sedimentation for Cell Pairing and Fusion |
| title_sort | microfluidic flip chip combining hydrodynamic trapping and gravitational sedimentation for cell pairing and fusion |
| topic | hydrodynamic trapping cell pairing dielectrophoresis cell fusion |
| url | https://www.mdpi.com/2073-4409/10/11/2855 |
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