Free-living bacteria stimulate sugarcane growth traits and edaphic factors along soil depth gradients under contrasting fertilization
Abstract Free-living bacterial community and abundance have been investigated extensively under different soil management practices. However, little is known about their nitrogen (N) fixation abilities, and how their contributions to N budgets impact plant growth, yield, and carbon (C) and N cycling...
| Main Authors: | , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Nature Portfolio
2023-04-01
|
| Series: | Scientific Reports |
| Online Access: | https://doi.org/10.1038/s41598-022-25807-w |
| _version_ | 1797841021593190400 |
|---|---|
| author | Nyumah Fallah Muhammad Tayyab Ziqi Yang Ziqin Pang Caifang Zhang Zhaoli Lin Lahand James Stewart Mbuya Sylvain Ntambo Ahmad Yusuf Abubakar Wenxiong Lin Hua Zhang |
| author_facet | Nyumah Fallah Muhammad Tayyab Ziqi Yang Ziqin Pang Caifang Zhang Zhaoli Lin Lahand James Stewart Mbuya Sylvain Ntambo Ahmad Yusuf Abubakar Wenxiong Lin Hua Zhang |
| author_sort | Nyumah Fallah |
| collection | DOAJ |
| description | Abstract Free-living bacterial community and abundance have been investigated extensively under different soil management practices. However, little is known about their nitrogen (N) fixation abilities, and how their contributions to N budgets impact plant growth, yield, and carbon (C) and N cycling enzymes in a long-term consecutive sugarcane monoculture farming system, under contrasting amendments, along different soil horizons. Here, nifH gene amplicon was used to investigate diazotrophs bacterial community and abundance by leveraging high-throughput sequencing (HTS). Moreover, edaphic factors in three soil depths (0–20, 20–40, and 40–60 cm) under control (CK), organic matter (OM), biochar (BC), and filter mud (FM) amended soils were investigated. Our analysis revealed that β-glucosidase activity, acid phosphatase activity, ammonium (NH4 +-N), nitrate (NO3 –N), total carbon (TC), total nitrogen (TN), and available potassium (AK) were considerably high in 0–20 cm in all the treatments. We also detected a significantly high proportion of Proteobacteria and Geobacter in the entire sample, including Anabaena and Enterobacter in 0–20 cm soil depth under the BC and FM amended soils, which we believed were worthy of promoting edaphic factors and sugarcane traits. This phenomenon was further reinforced by network analysis, where diazotrophs bacteria belonging to Proteobacteria exhibited strong and positive associations soil electrical conductivity (EC), soil organic matter content (SOM) available phosphorus (AP), TN, followed by NH4+-N and NO3 –N, a pattern that was further validated by Mantel test and Pearson’s correlation coefficients analyses. Furthermore, some potential N-fixing bacteria, including Burkholderia, Azotobacter, Anabaena, and Enterobacter exhibited a strong and positive association with sugarcane agronomic traits, namely, sugarcane stalk, ratoon weight, and chlorophyll content. Taken together, our findings are likely to broaden our understanding of free-living bacteria N-fixation abilities, and how their contributions to key soil nutrients such as N budgets impact plant growth and yield, including C and N cycling enzymes in a long-term consecutive sugarcane monoculture farming system, under contrasting amendments, along different soil horizons. |
| first_indexed | 2024-04-09T16:24:08Z |
| format | Article |
| id | doaj.art-a282448e8fb645a6ac3f81fde697e79c |
| institution | Directory Open Access Journal |
| issn | 2045-2322 |
| language | English |
| last_indexed | 2024-04-09T16:24:08Z |
| publishDate | 2023-04-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Reports |
| spelling | doaj.art-a282448e8fb645a6ac3f81fde697e79c2023-04-23T11:17:03ZengNature PortfolioScientific Reports2045-23222023-04-0113111410.1038/s41598-022-25807-wFree-living bacteria stimulate sugarcane growth traits and edaphic factors along soil depth gradients under contrasting fertilizationNyumah Fallah0Muhammad Tayyab1Ziqi Yang2Ziqin Pang3Caifang Zhang4Zhaoli Lin5Lahand James Stewart6Mbuya Sylvain Ntambo7Ahmad Yusuf Abubakar8Wenxiong Lin9Hua Zhang10Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry UniversityKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry UniversityKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry UniversityKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry UniversityKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry UniversityKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry UniversityKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry UniversityDépartement de Phytotechnie, Faculté des Sciences Agronominiques, Université de KolweziKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry UniversityFujian Provincial Key Laboratory of Agro-Ecological Processing and Safety Monitoring, College of Life Sciences, Fujian Agriculture and Forestry UniversityKey Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture, Fujian Agriculture and Forestry UniversityAbstract Free-living bacterial community and abundance have been investigated extensively under different soil management practices. However, little is known about their nitrogen (N) fixation abilities, and how their contributions to N budgets impact plant growth, yield, and carbon (C) and N cycling enzymes in a long-term consecutive sugarcane monoculture farming system, under contrasting amendments, along different soil horizons. Here, nifH gene amplicon was used to investigate diazotrophs bacterial community and abundance by leveraging high-throughput sequencing (HTS). Moreover, edaphic factors in three soil depths (0–20, 20–40, and 40–60 cm) under control (CK), organic matter (OM), biochar (BC), and filter mud (FM) amended soils were investigated. Our analysis revealed that β-glucosidase activity, acid phosphatase activity, ammonium (NH4 +-N), nitrate (NO3 –N), total carbon (TC), total nitrogen (TN), and available potassium (AK) were considerably high in 0–20 cm in all the treatments. We also detected a significantly high proportion of Proteobacteria and Geobacter in the entire sample, including Anabaena and Enterobacter in 0–20 cm soil depth under the BC and FM amended soils, which we believed were worthy of promoting edaphic factors and sugarcane traits. This phenomenon was further reinforced by network analysis, where diazotrophs bacteria belonging to Proteobacteria exhibited strong and positive associations soil electrical conductivity (EC), soil organic matter content (SOM) available phosphorus (AP), TN, followed by NH4+-N and NO3 –N, a pattern that was further validated by Mantel test and Pearson’s correlation coefficients analyses. Furthermore, some potential N-fixing bacteria, including Burkholderia, Azotobacter, Anabaena, and Enterobacter exhibited a strong and positive association with sugarcane agronomic traits, namely, sugarcane stalk, ratoon weight, and chlorophyll content. Taken together, our findings are likely to broaden our understanding of free-living bacteria N-fixation abilities, and how their contributions to key soil nutrients such as N budgets impact plant growth and yield, including C and N cycling enzymes in a long-term consecutive sugarcane monoculture farming system, under contrasting amendments, along different soil horizons.https://doi.org/10.1038/s41598-022-25807-w |
| spellingShingle | Nyumah Fallah Muhammad Tayyab Ziqi Yang Ziqin Pang Caifang Zhang Zhaoli Lin Lahand James Stewart Mbuya Sylvain Ntambo Ahmad Yusuf Abubakar Wenxiong Lin Hua Zhang Free-living bacteria stimulate sugarcane growth traits and edaphic factors along soil depth gradients under contrasting fertilization Scientific Reports |
| title | Free-living bacteria stimulate sugarcane growth traits and edaphic factors along soil depth gradients under contrasting fertilization |
| title_full | Free-living bacteria stimulate sugarcane growth traits and edaphic factors along soil depth gradients under contrasting fertilization |
| title_fullStr | Free-living bacteria stimulate sugarcane growth traits and edaphic factors along soil depth gradients under contrasting fertilization |
| title_full_unstemmed | Free-living bacteria stimulate sugarcane growth traits and edaphic factors along soil depth gradients under contrasting fertilization |
| title_short | Free-living bacteria stimulate sugarcane growth traits and edaphic factors along soil depth gradients under contrasting fertilization |
| title_sort | free living bacteria stimulate sugarcane growth traits and edaphic factors along soil depth gradients under contrasting fertilization |
| url | https://doi.org/10.1038/s41598-022-25807-w |
| work_keys_str_mv | AT nyumahfallah freelivingbacteriastimulatesugarcanegrowthtraitsandedaphicfactorsalongsoildepthgradientsundercontrastingfertilization AT muhammadtayyab freelivingbacteriastimulatesugarcanegrowthtraitsandedaphicfactorsalongsoildepthgradientsundercontrastingfertilization AT ziqiyang freelivingbacteriastimulatesugarcanegrowthtraitsandedaphicfactorsalongsoildepthgradientsundercontrastingfertilization AT ziqinpang freelivingbacteriastimulatesugarcanegrowthtraitsandedaphicfactorsalongsoildepthgradientsundercontrastingfertilization AT caifangzhang freelivingbacteriastimulatesugarcanegrowthtraitsandedaphicfactorsalongsoildepthgradientsundercontrastingfertilization AT zhaolilin freelivingbacteriastimulatesugarcanegrowthtraitsandedaphicfactorsalongsoildepthgradientsundercontrastingfertilization AT lahandjamesstewart freelivingbacteriastimulatesugarcanegrowthtraitsandedaphicfactorsalongsoildepthgradientsundercontrastingfertilization AT mbuyasylvainntambo freelivingbacteriastimulatesugarcanegrowthtraitsandedaphicfactorsalongsoildepthgradientsundercontrastingfertilization AT ahmadyusufabubakar freelivingbacteriastimulatesugarcanegrowthtraitsandedaphicfactorsalongsoildepthgradientsundercontrastingfertilization AT wenxionglin freelivingbacteriastimulatesugarcanegrowthtraitsandedaphicfactorsalongsoildepthgradientsundercontrastingfertilization AT huazhang freelivingbacteriastimulatesugarcanegrowthtraitsandedaphicfactorsalongsoildepthgradientsundercontrastingfertilization |