A duplex droplet digital PCR assay for Salmonella and Shigella and its application in diarrheal and non-diarrheal samples

A duplex droplet digital PCR assay for Salmonella and Shigella and its application in diarrheal and non-diarrheal samples

Objectives: To evaluate a duplex droplet digital polymerase chain reaction (ddPCR) assay targeting Salmonella fimY and Shigella ipaH genes. Methods: The linear range, precision, analytical sensitivity, and analytical specificity of the ddPCR assay were analyzed. The ddPCR assay was compared with qua...

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Bibliographic Details
Main Authors:	Jingyun Zhang, Yuanming Huang, Panpan Xue, Zhifei Zhan, Zheng Huang, Jie Li, Baowei Diao, Biao Kan
Format:	Article
Language:	English
Published:	Elsevier 2022-07-01
Series:	International Journal of Infectious Diseases
Subjects:	Salmonella Shigella Droplet digital PCR Real-time PCR Children with diarrhea Children without diarrhea
Online Access:	http://www.sciencedirect.com/science/article/pii/S1201971222002429

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