UVA-Degradable Collagenase Nanocapsules as a Potential Treatment for Fibrotic Diseases
Peyronie and Dupuytren are pathologies characterized by the appearance of localized fibrotic lesions in an organ. These disorders originate from an excessive production of collagen in the tissue provoking dysfunction and functional limitations to the patients. Local administration of collagenase is...
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| Format: | Article |
| Language: | English |
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MDPI AG
2021-04-01
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| Series: | Pharmaceutics |
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| Online Access: | https://www.mdpi.com/1999-4923/13/4/499 |
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| author | Víctor M. Moreno Carolina Meroño Alejandro Baeza Alicia Usategui Pablo L. Ortiz-Romero José L. Pablos María Vallet-Regí |
| author_facet | Víctor M. Moreno Carolina Meroño Alejandro Baeza Alicia Usategui Pablo L. Ortiz-Romero José L. Pablos María Vallet-Regí |
| author_sort | Víctor M. Moreno |
| collection | DOAJ |
| description | Peyronie and Dupuytren are pathologies characterized by the appearance of localized fibrotic lesions in an organ. These disorders originate from an excessive production of collagen in the tissue provoking dysfunction and functional limitations to the patients. Local administration of collagenase is the most used treatment for these fibrotic-type diseases, but a high lability of the enzyme limits its therapeutic efficacy. Herein, we present a novel methodology for the preparation of collagenase nanocapsules without affecting its enzymatic activity and capable of releasing the enzyme in response to an ultraviolet A (UVA) light stimulus. Polymeric coating around collagenase was formed by free-radical polymerization of acrylamide-type monomers. Their degradation capacity under UVA irradiation was provided by incorporating a novel photocleavable acrylamide-type crosslinker within the polymeric framework. This property allowed collagenase release to be triggered in a controlled manner by employing an easily focused stimulus. Additionally, UVA irradiation presents considerable benefits by itself due to its capacity to induce collagenase production in situ. An expected synergistic effect of collagenase nanocapsules in conjunction with UVA effect may present a promising treatment for these fibrotic diseases. |
| first_indexed | 2024-03-10T12:34:04Z |
| format | Article |
| id | doaj.art-a2858a2a0e284996895afe1208c536af |
| institution | Directory Open Access Journal |
| issn | 1999-4923 |
| language | English |
| last_indexed | 2024-03-10T12:34:04Z |
| publishDate | 2021-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pharmaceutics |
| spelling | doaj.art-a2858a2a0e284996895afe1208c536af2023-11-21T14:24:25ZengMDPI AGPharmaceutics1999-49232021-04-0113449910.3390/pharmaceutics13040499UVA-Degradable Collagenase Nanocapsules as a Potential Treatment for Fibrotic DiseasesVíctor M. Moreno0Carolina Meroño1Alejandro Baeza2Alicia Usategui3Pablo L. Ortiz-Romero4José L. Pablos5María Vallet-Regí6Departamento de Química en Ciencias Farmacéuticas, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria, Hospital 12 de Octubre i+12, Plaza Ramón y Cajal s/n, 28040 Madrid, SpainServicio de Reumatología, Instituto de Investigación Hospital 12 de Octubre (i+12), Universidad Complutense de Madrid, Avenida Córdoba s/n, 28041 Madrid, SpainDepartamento de Materiales y Producción Aeroespacial, ETSI Aeronáutica y del Espacio, Universidad Politécnica de Madrid, 28040 Madrid, SpainServicio de Reumatología, Instituto de Investigación Hospital 12 de Octubre (i+12), Universidad Complutense de Madrid, Avenida Córdoba s/n, 28041 Madrid, SpainServicio de Dermatología, Hospital 12 de Octubre, Instituto (i+12 Medical School), Universidad Complutense de Madrid, Avenida Córdoba s/n, 28041 Madrid, SpainServicio de Reumatología, Instituto de Investigación Hospital 12 de Octubre (i+12), Universidad Complutense de Madrid, Avenida Córdoba s/n, 28041 Madrid, SpainDepartamento de Química en Ciencias Farmacéuticas, Universidad Complutense de Madrid, Instituto de Investigación Sanitaria, Hospital 12 de Octubre i+12, Plaza Ramón y Cajal s/n, 28040 Madrid, SpainPeyronie and Dupuytren are pathologies characterized by the appearance of localized fibrotic lesions in an organ. These disorders originate from an excessive production of collagen in the tissue provoking dysfunction and functional limitations to the patients. Local administration of collagenase is the most used treatment for these fibrotic-type diseases, but a high lability of the enzyme limits its therapeutic efficacy. Herein, we present a novel methodology for the preparation of collagenase nanocapsules without affecting its enzymatic activity and capable of releasing the enzyme in response to an ultraviolet A (UVA) light stimulus. Polymeric coating around collagenase was formed by free-radical polymerization of acrylamide-type monomers. Their degradation capacity under UVA irradiation was provided by incorporating a novel photocleavable acrylamide-type crosslinker within the polymeric framework. This property allowed collagenase release to be triggered in a controlled manner by employing an easily focused stimulus. Additionally, UVA irradiation presents considerable benefits by itself due to its capacity to induce collagenase production in situ. An expected synergistic effect of collagenase nanocapsules in conjunction with UVA effect may present a promising treatment for these fibrotic diseases.https://www.mdpi.com/1999-4923/13/4/499photolinkercollagenase nanocapsulesfibrotic diseasescollagenUVA |
| spellingShingle | Víctor M. Moreno Carolina Meroño Alejandro Baeza Alicia Usategui Pablo L. Ortiz-Romero José L. Pablos María Vallet-Regí UVA-Degradable Collagenase Nanocapsules as a Potential Treatment for Fibrotic Diseases Pharmaceutics photolinker collagenase nanocapsules fibrotic diseases collagen UVA |
| title | UVA-Degradable Collagenase Nanocapsules as a Potential Treatment for Fibrotic Diseases |
| title_full | UVA-Degradable Collagenase Nanocapsules as a Potential Treatment for Fibrotic Diseases |
| title_fullStr | UVA-Degradable Collagenase Nanocapsules as a Potential Treatment for Fibrotic Diseases |
| title_full_unstemmed | UVA-Degradable Collagenase Nanocapsules as a Potential Treatment for Fibrotic Diseases |
| title_short | UVA-Degradable Collagenase Nanocapsules as a Potential Treatment for Fibrotic Diseases |
| title_sort | uva degradable collagenase nanocapsules as a potential treatment for fibrotic diseases |
| topic | photolinker collagenase nanocapsules fibrotic diseases collagen UVA |
| url | https://www.mdpi.com/1999-4923/13/4/499 |
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