Construction of Alizarin Conjugated Graphene Oxide Composites for Inhibition of <i>Candida albicans</i> Biofilms
Biofilm inhibition using nanoparticle-based drug carriers has emerged as a noninvasive strategy to eradicate microbial contaminants such as fungus <i>Candida albicans</i>. In this study, one-step adsorption strategy was utilized to conjugate alizarin (AZ) on graphene oxide (GO) and chara...
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| Format: | Article |
| Language: | English |
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MDPI AG
2020-04-01
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| Series: | Biomolecules |
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| Online Access: | https://www.mdpi.com/2218-273X/10/4/565 |
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| author | Mohankandhasamy Ramasamy Sitansu Sekhar Nanda Jin-Hyung Lee Jintae Lee |
| author_facet | Mohankandhasamy Ramasamy Sitansu Sekhar Nanda Jin-Hyung Lee Jintae Lee |
| author_sort | Mohankandhasamy Ramasamy |
| collection | DOAJ |
| description | Biofilm inhibition using nanoparticle-based drug carriers has emerged as a noninvasive strategy to eradicate microbial contaminants such as fungus <i>Candida albicans</i>. In this study, one-step adsorption strategy was utilized to conjugate alizarin (AZ) on graphene oxide (GO) and characterized by ultraviolet-visible spectroscopy (UV-Vis), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray powder diffraction (XRD), dynamic light-scattering (DLS), and transmission electron microscopy (TEM). Crystal violet assay was performed to evaluate the antibiofilm efficacy of GO-AZs against <i>C. albicans</i>. Different characterizations disclosed the loading of AZ onto GO. Interestingly, TEM images indicated the abundant loading of AZ by producing a unique inward rolling of GO-AZ sheets as compared to GO. When compared to the nontreatment, GO-AZ at 10 µg/mL significantly reduced biofilm formation to 96% almost equal to the amount of AZ (95%). It appears that the biofilm inhibition is due to the hyphal inhibition of <i>C. albicans</i>. The GO is an interesting nanocarrier for loading AZ and could be applied as a novel antibiofilm agent against various microorganisms including <i>C. albicans</i>. |
| first_indexed | 2024-03-10T20:38:02Z |
| format | Article |
| id | doaj.art-a289954c1c0d41cc965292cfc0ce7255 |
| institution | Directory Open Access Journal |
| issn | 2218-273X |
| language | English |
| last_indexed | 2024-03-10T20:38:02Z |
| publishDate | 2020-04-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Biomolecules |
| spelling | doaj.art-a289954c1c0d41cc965292cfc0ce72552023-11-19T20:55:05ZengMDPI AGBiomolecules2218-273X2020-04-0110456510.3390/biom10040565Construction of Alizarin Conjugated Graphene Oxide Composites for Inhibition of <i>Candida albicans</i> BiofilmsMohankandhasamy Ramasamy0Sitansu Sekhar Nanda1Jin-Hyung Lee2Jintae Lee3School of Chemical Engineering, Yeungnam University, Gyeongsan 38541, KoreaDepartment of Chemistry, Myongji University, Yongin 449728, KoreaSchool of Chemical Engineering, Yeungnam University, Gyeongsan 38541, KoreaSchool of Chemical Engineering, Yeungnam University, Gyeongsan 38541, KoreaBiofilm inhibition using nanoparticle-based drug carriers has emerged as a noninvasive strategy to eradicate microbial contaminants such as fungus <i>Candida albicans</i>. In this study, one-step adsorption strategy was utilized to conjugate alizarin (AZ) on graphene oxide (GO) and characterized by ultraviolet-visible spectroscopy (UV-Vis), attenuated total reflectance Fourier transform infrared spectroscopy (ATR-FTIR), X-ray powder diffraction (XRD), dynamic light-scattering (DLS), and transmission electron microscopy (TEM). Crystal violet assay was performed to evaluate the antibiofilm efficacy of GO-AZs against <i>C. albicans</i>. Different characterizations disclosed the loading of AZ onto GO. Interestingly, TEM images indicated the abundant loading of AZ by producing a unique inward rolling of GO-AZ sheets as compared to GO. When compared to the nontreatment, GO-AZ at 10 µg/mL significantly reduced biofilm formation to 96% almost equal to the amount of AZ (95%). It appears that the biofilm inhibition is due to the hyphal inhibition of <i>C. albicans</i>. The GO is an interesting nanocarrier for loading AZ and could be applied as a novel antibiofilm agent against various microorganisms including <i>C. albicans</i>. https://www.mdpi.com/2218-273X/10/4/565adsorptiongraphene oxidealizarinantibiofilm<i>C. albicans</i>hyphal inhibition |
| spellingShingle | Mohankandhasamy Ramasamy Sitansu Sekhar Nanda Jin-Hyung Lee Jintae Lee Construction of Alizarin Conjugated Graphene Oxide Composites for Inhibition of <i>Candida albicans</i> Biofilms Biomolecules adsorption graphene oxide alizarin antibiofilm <i>C. albicans</i> hyphal inhibition |
| title | Construction of Alizarin Conjugated Graphene Oxide Composites for Inhibition of <i>Candida albicans</i> Biofilms |
| title_full | Construction of Alizarin Conjugated Graphene Oxide Composites for Inhibition of <i>Candida albicans</i> Biofilms |
| title_fullStr | Construction of Alizarin Conjugated Graphene Oxide Composites for Inhibition of <i>Candida albicans</i> Biofilms |
| title_full_unstemmed | Construction of Alizarin Conjugated Graphene Oxide Composites for Inhibition of <i>Candida albicans</i> Biofilms |
| title_short | Construction of Alizarin Conjugated Graphene Oxide Composites for Inhibition of <i>Candida albicans</i> Biofilms |
| title_sort | construction of alizarin conjugated graphene oxide composites for inhibition of i candida albicans i biofilms |
| topic | adsorption graphene oxide alizarin antibiofilm <i>C. albicans</i> hyphal inhibition |
| url | https://www.mdpi.com/2218-273X/10/4/565 |
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