Median lethal needle caliber in two models of experimental sepsis
PURPOSE: To estimate the median lethal needle caliber (LC50) of a new experimental sepsis model and compare it to the LC50 of the cecal ligation and puncture (CLP) sepsis model. METHODS: Male albino Wistar rats were studied (n=22). Animals were allocated into two study groups. In Group I, exper...
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| Format: | Article |
| Language: | English |
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Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia
2014-01-01
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| Series: | Acta Cirúrgica Brasileira |
| Subjects: | |
| Online Access: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000100001&tlng=en |
| _version_ | 1819279778785525760 |
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| author | Armando Jose d'Acampora Giovani de Figueiredo Locks |
| author_facet | Armando Jose d'Acampora Giovani de Figueiredo Locks |
| author_sort | Armando Jose d'Acampora |
| collection | DOAJ |
| description | PURPOSE: To estimate the median lethal needle caliber (LC50) of a new experimental sepsis model and compare it to the LC50 of the cecal ligation and puncture (CLP) sepsis model. METHODS: Male albino Wistar rats were studied (n=22). Animals were allocated into two study groups. In Group I, experimental sepsis was induced by cecal ligation and puncture. In Group II, experimental sepsis was induced by ascending colon ligation and cecal puncture. Up-and-down method was used to determinate the LC50. RESULTS: LC50 in Group I was 19 Gauge (Confidence Interval 17 to 22 Gauge). Determination of LC50 was not possible in Group II due to the death of all animals. CONCLUSION: LC50 in cecal ligation and puncture is 19 Gauge. The lethality of the new model tested in this trial is very high. |
| first_indexed | 2024-12-24T00:33:19Z |
| format | Article |
| id | doaj.art-a28aab5350e24faaa37d984e14199b96 |
| institution | Directory Open Access Journal |
| issn | 0102-8650 |
| language | English |
| last_indexed | 2024-12-24T00:33:19Z |
| publishDate | 2014-01-01 |
| publisher | Sociedade Brasileira para o Desenvolvimento da Pesquisa em Cirurgia |
| record_format | Article |
| series | Acta Cirúrgica Brasileira |
| spelling | doaj.art-a28aab5350e24faaa37d984e14199b962022-12-21T17:24:10ZengSociedade Brasileira para o Desenvolvimento da Pesquisa em CirurgiaActa Cirúrgica Brasileira0102-86502014-01-01291010610.1590/S0102-86502014000100001Median lethal needle caliber in two models of experimental sepsisArmando Jose d'AcamporaGiovani de Figueiredo LocksPURPOSE: To estimate the median lethal needle caliber (LC50) of a new experimental sepsis model and compare it to the LC50 of the cecal ligation and puncture (CLP) sepsis model. METHODS: Male albino Wistar rats were studied (n=22). Animals were allocated into two study groups. In Group I, experimental sepsis was induced by cecal ligation and puncture. In Group II, experimental sepsis was induced by ascending colon ligation and cecal puncture. Up-and-down method was used to determinate the LC50. RESULTS: LC50 in Group I was 19 Gauge (Confidence Interval 17 to 22 Gauge). Determination of LC50 was not possible in Group II due to the death of all animals. CONCLUSION: LC50 in cecal ligation and puncture is 19 Gauge. The lethality of the new model tested in this trial is very high.http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000100001&tlng=enSepsisDisease Models, AnimalRats |
| spellingShingle | Armando Jose d'Acampora Giovani de Figueiredo Locks Median lethal needle caliber in two models of experimental sepsis Acta Cirúrgica Brasileira Sepsis Disease Models, Animal Rats |
| title | Median lethal needle caliber in two models of experimental sepsis |
| title_full | Median lethal needle caliber in two models of experimental sepsis |
| title_fullStr | Median lethal needle caliber in two models of experimental sepsis |
| title_full_unstemmed | Median lethal needle caliber in two models of experimental sepsis |
| title_short | Median lethal needle caliber in two models of experimental sepsis |
| title_sort | median lethal needle caliber in two models of experimental sepsis |
| topic | Sepsis Disease Models, Animal Rats |
| url | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-86502014000100001&tlng=en |
| work_keys_str_mv | AT armandojosedacampora medianlethalneedlecaliberintwomodelsofexperimentalsepsis AT giovanidefigueiredolocks medianlethalneedlecaliberintwomodelsofexperimentalsepsis |