Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactyla mixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae)
Sea cucumber taxonomy and systematics has in the past heavily relied on gross external and internal anatomy, ossicle assemblage in different tissues, and molecular characterisation, with coloration, habitat, and geographical and bathymethric distribution also considered important parameters. In the...
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| Format: | Article |
| Language: | English |
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Pensoft Publishers
2021-08-01
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| Series: | ZooKeys |
| Online Access: | https://zookeys.pensoft.net/article/67088/download/pdf/ |
| _version_ | 1811329279248564224 |
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| author | Yves Samyn Gontran Sonet Cedric d'Udekem d'Acoz |
| author_facet | Yves Samyn Gontran Sonet Cedric d'Udekem d'Acoz |
| author_sort | Yves Samyn |
| collection | DOAJ |
| description | Sea cucumber taxonomy and systematics has in the past heavily relied on gross external and internal anatomy, ossicle assemblage in different tissues, and molecular characterisation, with coloration, habitat, and geographical and bathymethric distribution also considered important parameters. In the present paper, we made these observations and techniques in detail and complemented them with the novel technique of micro-computed tomography of the calcareous ring. We investigated a single European species, the so-called gravel sea cucumber, Neopentadactyla mixta (Östergren, 1898), using recently collected material from the Chausey Islands, Normandy, France. We redescribed the species, illustrated its ossicle assemblage through scanning electron microscopy, and visualised the calcareous ring through stacking photography and through micro-CT scanning. Additionally, a DNA fragment of 955 base pairs of the 18S ribosomal RNA gene was sequenced from one specimen, which showed a high similarity with the only sequence of N. mixta publicly available. We completed this integrative study by providing a detailed distribution of the occurrence of N. mixta based on published, verifiable accounts. |
| first_indexed | 2024-04-13T15:41:00Z |
| format | Article |
| id | doaj.art-a28addcc413e4a2cb54969d5ba81e3c1 |
| institution | Directory Open Access Journal |
| issn | 1313-2970 |
| language | English |
| last_indexed | 2024-04-13T15:41:00Z |
| publishDate | 2021-08-01 |
| publisher | Pensoft Publishers |
| record_format | Article |
| series | ZooKeys |
| spelling | doaj.art-a28addcc413e4a2cb54969d5ba81e3c12022-12-22T02:41:09ZengPensoft PublishersZooKeys1313-29702021-08-01105417318410.3897/zookeys.1054.6708867088Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactyla mixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae)Yves Samyn0Gontran Sonet1Cedric d'Udekem d'Acoz2Scientific Service of Patrimony, Royal Belgian Institute of Natural SciencesJoint Experimental Molecular Unit, Royal Belgian Institute of Natural SciencesScientific Service of Patrimony, Royal Belgian Institute of Natural SciencesSea cucumber taxonomy and systematics has in the past heavily relied on gross external and internal anatomy, ossicle assemblage in different tissues, and molecular characterisation, with coloration, habitat, and geographical and bathymethric distribution also considered important parameters. In the present paper, we made these observations and techniques in detail and complemented them with the novel technique of micro-computed tomography of the calcareous ring. We investigated a single European species, the so-called gravel sea cucumber, Neopentadactyla mixta (Östergren, 1898), using recently collected material from the Chausey Islands, Normandy, France. We redescribed the species, illustrated its ossicle assemblage through scanning electron microscopy, and visualised the calcareous ring through stacking photography and through micro-CT scanning. Additionally, a DNA fragment of 955 base pairs of the 18S ribosomal RNA gene was sequenced from one specimen, which showed a high similarity with the only sequence of N. mixta publicly available. We completed this integrative study by providing a detailed distribution of the occurrence of N. mixta based on published, verifiable accounts.https://zookeys.pensoft.net/article/67088/download/pdf/ |
| spellingShingle | Yves Samyn Gontran Sonet Cedric d'Udekem d'Acoz Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactyla mixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae) ZooKeys |
| title | Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactyla mixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae) |
| title_full | Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactyla mixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae) |
| title_fullStr | Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactyla mixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae) |
| title_full_unstemmed | Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactyla mixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae) |
| title_short | Exploring the use of micro-computed tomography (micro-CT) in the taxonomy of sea cucumbers: a case-study on the gravel sea cucumber Neopentadactyla mixta (Östergren, 1898) (Echinodermata, Holothuroidea, Phyllophoridae) |
| title_sort | exploring the use of micro computed tomography micro ct in the taxonomy of sea cucumbers a case study on the gravel sea cucumber neopentadactyla mixta ostergren 1898 echinodermata holothuroidea phyllophoridae |
| url | https://zookeys.pensoft.net/article/67088/download/pdf/ |
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