Scopolin Prevents Adipocyte Differentiation in 3T3-L1 Preadipocytes and Weight Gain in an Ovariectomy-Induced Obese Mouse Model
Obesity is prevalent in modern human societies. We examined the anti-obesity effects of scopolin on adipocyte differentiation in preadipocyte 3T3-L1 cells and weight loss in an ovariectomy (OVX)-induced obese mouse model. Scopolin inhibited adipocyte differentiation and lipid accumulation in the pre...
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MDPI AG
2020-11-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/21/22/8699 |
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| author | Eunkuk Park Chang Gun Lee Jeonghyun Kim Eunguk Lim Subin Yeo Seon-Yong Jeong |
| author_facet | Eunkuk Park Chang Gun Lee Jeonghyun Kim Eunguk Lim Subin Yeo Seon-Yong Jeong |
| author_sort | Eunkuk Park |
| collection | DOAJ |
| description | Obesity is prevalent in modern human societies. We examined the anti-obesity effects of scopolin on adipocyte differentiation in preadipocyte 3T3-L1 cells and weight loss in an ovariectomy (OVX)-induced obese mouse model. Scopolin inhibited adipocyte differentiation and lipid accumulation in the preadipocyte cells by suppressing the transcription of adipogenic-related factors, including adiponectin (<i>Adipoq</i>), peroxisome proliferator-activated receptor gamma (<i>Pparg</i>), lipoprotein lipase (<i>Lpl</i>), perilipin1 (<i>Plin1</i>), fatty acid-binding protein 4 (<i>Fabp4</i>), glucose transporter type 4 (<i>Slc2a4</i>), and CCAAT/enhancer-binding protein alpha (<i>Cebpa</i>). In OVX-induced obese mice, administration of scopolin promoted the reduction of body weight, total fat percentage, liver steatosis, and adipose cell size. In addition, the scopolin-treated OVX mice showed decreased serum levels of leptin and insulin. Taken together, these findings suggest that the use of scopolin prevented adipocyte differentiation and weight gain in vitro and in vivo, indicating that scopolin may be a potential bioactive compound for the treatment and prevention of obesity in humans. |
| first_indexed | 2024-03-10T14:46:01Z |
| format | Article |
| id | doaj.art-a28d95da97cc4d6898230506dcf56629 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-10T14:46:01Z |
| publishDate | 2020-11-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a28d95da97cc4d6898230506dcf566292023-11-20T21:24:00ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672020-11-012122869910.3390/ijms21228699Scopolin Prevents Adipocyte Differentiation in 3T3-L1 Preadipocytes and Weight Gain in an Ovariectomy-Induced Obese Mouse ModelEunkuk Park0Chang Gun Lee1Jeonghyun Kim2Eunguk Lim3Subin Yeo4Seon-Yong Jeong5Department of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaDepartment of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaNine B Company, Daejeon 34121, KoreaDepartment of Medical Genetics, Ajou University School of Medicine, Suwon 16499, KoreaObesity is prevalent in modern human societies. We examined the anti-obesity effects of scopolin on adipocyte differentiation in preadipocyte 3T3-L1 cells and weight loss in an ovariectomy (OVX)-induced obese mouse model. Scopolin inhibited adipocyte differentiation and lipid accumulation in the preadipocyte cells by suppressing the transcription of adipogenic-related factors, including adiponectin (<i>Adipoq</i>), peroxisome proliferator-activated receptor gamma (<i>Pparg</i>), lipoprotein lipase (<i>Lpl</i>), perilipin1 (<i>Plin1</i>), fatty acid-binding protein 4 (<i>Fabp4</i>), glucose transporter type 4 (<i>Slc2a4</i>), and CCAAT/enhancer-binding protein alpha (<i>Cebpa</i>). In OVX-induced obese mice, administration of scopolin promoted the reduction of body weight, total fat percentage, liver steatosis, and adipose cell size. In addition, the scopolin-treated OVX mice showed decreased serum levels of leptin and insulin. Taken together, these findings suggest that the use of scopolin prevented adipocyte differentiation and weight gain in vitro and in vivo, indicating that scopolin may be a potential bioactive compound for the treatment and prevention of obesity in humans.https://www.mdpi.com/1422-0067/21/22/8699obesityscopolinadipogenesisOVX-induced obese miceanti-obesity effect |
| spellingShingle | Eunkuk Park Chang Gun Lee Jeonghyun Kim Eunguk Lim Subin Yeo Seon-Yong Jeong Scopolin Prevents Adipocyte Differentiation in 3T3-L1 Preadipocytes and Weight Gain in an Ovariectomy-Induced Obese Mouse Model International Journal of Molecular Sciences obesity scopolin adipogenesis OVX-induced obese mice anti-obesity effect |
| title | Scopolin Prevents Adipocyte Differentiation in 3T3-L1 Preadipocytes and Weight Gain in an Ovariectomy-Induced Obese Mouse Model |
| title_full | Scopolin Prevents Adipocyte Differentiation in 3T3-L1 Preadipocytes and Weight Gain in an Ovariectomy-Induced Obese Mouse Model |
| title_fullStr | Scopolin Prevents Adipocyte Differentiation in 3T3-L1 Preadipocytes and Weight Gain in an Ovariectomy-Induced Obese Mouse Model |
| title_full_unstemmed | Scopolin Prevents Adipocyte Differentiation in 3T3-L1 Preadipocytes and Weight Gain in an Ovariectomy-Induced Obese Mouse Model |
| title_short | Scopolin Prevents Adipocyte Differentiation in 3T3-L1 Preadipocytes and Weight Gain in an Ovariectomy-Induced Obese Mouse Model |
| title_sort | scopolin prevents adipocyte differentiation in 3t3 l1 preadipocytes and weight gain in an ovariectomy induced obese mouse model |
| topic | obesity scopolin adipogenesis OVX-induced obese mice anti-obesity effect |
| url | https://www.mdpi.com/1422-0067/21/22/8699 |
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