<i>Bombyx mori</i> Nucleopolyhedrovirus <i>p26</i> Is Associated with Viral Late Stage Replication
<i>Bombyx mori</i> nucleopolyhedrovirus (BmNPV) <i>p26</i> is conserved among all Lepidoptera baculoviruses that have been completely sequenced thus far, and some baculoviruses even have two copies of <i>p26</i>, which suggested that <i>p26</i> may pla...
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MDPI AG
2021-08-01
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| Series: | Insects |
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| Online Access: | https://www.mdpi.com/2075-4450/12/8/707 |
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| author | Jun-Qing Ge Zhu-Hong Wang Xi Chen Hua Chen Jian Huang |
| author_facet | Jun-Qing Ge Zhu-Hong Wang Xi Chen Hua Chen Jian Huang |
| author_sort | Jun-Qing Ge |
| collection | DOAJ |
| description | <i>Bombyx mori</i> nucleopolyhedrovirus (BmNPV) <i>p26</i> is conserved among all Lepidoptera baculoviruses that have been completely sequenced thus far, and some baculoviruses even have two copies of <i>p26</i>, which suggested that <i>p26</i> may play an important role in the virus infection cycle. This study aimed to characterize BmNPV <i>p26</i>. We found that BmNPV <i>p26</i> transcripts were detectable as early as 3 h post-infection (hpi), and the transcript levels rapidly increased starting from 12 hpi. Western blot analysis using an anti-<i>p26</i> polyclonal antibody demonstrated that the corresponding protein was also detectable from 6 hpi in BmNPV-infected cell lysates. Immunofluorescence analysis demonstrated that <i>p26</i> was mainly dispersed in the infected cell cytoplasm, whereas the over-expressed fusion protein EGFP-p26 also accumulated in the nucleus. These results indicated that <i>p26</i> is an early BmNPV gene and has functions both in the cytoplasm and the nucleus. RNAi-based knockdown of <i>p26</i> could produce infectious virus and normal-appearing virions but decreased budded virus (BV) production in BmNPV-infected cells at 72 hpi. Moreover, the results of further quantitative PCR (Q-PCR) analysis indicated that the <i>gp64</i> and <i>p74</i> transcripts levels decreased significantly. These results indicated that BmNPV <i>p26</i> may be associated with BmNPV replication during the late infection stage. |
| first_indexed | 2024-03-10T08:43:09Z |
| format | Article |
| id | doaj.art-a28f606f7d35428aad784dd9fb6b477c |
| institution | Directory Open Access Journal |
| issn | 2075-4450 |
| language | English |
| last_indexed | 2024-03-10T08:43:09Z |
| publishDate | 2021-08-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Insects |
| spelling | doaj.art-a28f606f7d35428aad784dd9fb6b477c2023-11-22T08:07:22ZengMDPI AGInsects2075-44502021-08-0112870710.3390/insects12080707<i>Bombyx mori</i> Nucleopolyhedrovirus <i>p26</i> Is Associated with Viral Late Stage ReplicationJun-Qing Ge0Zhu-Hong Wang1Xi Chen2Hua Chen3Jian Huang4Institute of Biotechnology, Fujian Academy of Agricultural Sciences, Wusi Road 247, Fuzhou 350003, ChinaDepartment of Entomology, College of Plant Protection, Fujian Agriculture and Forestry University, Shangxiadian Road 15, Fuzhou 350002, ChinaInstitute of Biotechnology, Fujian Academy of Agricultural Sciences, Wusi Road 247, Fuzhou 350003, ChinaInstitute of Biotechnology, Fujian Academy of Agricultural Sciences, Wusi Road 247, Fuzhou 350003, ChinaDepartment of Entomology, College of Plant Protection, Fujian Agriculture and Forestry University, Shangxiadian Road 15, Fuzhou 350002, China<i>Bombyx mori</i> nucleopolyhedrovirus (BmNPV) <i>p26</i> is conserved among all Lepidoptera baculoviruses that have been completely sequenced thus far, and some baculoviruses even have two copies of <i>p26</i>, which suggested that <i>p26</i> may play an important role in the virus infection cycle. This study aimed to characterize BmNPV <i>p26</i>. We found that BmNPV <i>p26</i> transcripts were detectable as early as 3 h post-infection (hpi), and the transcript levels rapidly increased starting from 12 hpi. Western blot analysis using an anti-<i>p26</i> polyclonal antibody demonstrated that the corresponding protein was also detectable from 6 hpi in BmNPV-infected cell lysates. Immunofluorescence analysis demonstrated that <i>p26</i> was mainly dispersed in the infected cell cytoplasm, whereas the over-expressed fusion protein EGFP-p26 also accumulated in the nucleus. These results indicated that <i>p26</i> is an early BmNPV gene and has functions both in the cytoplasm and the nucleus. RNAi-based knockdown of <i>p26</i> could produce infectious virus and normal-appearing virions but decreased budded virus (BV) production in BmNPV-infected cells at 72 hpi. Moreover, the results of further quantitative PCR (Q-PCR) analysis indicated that the <i>gp64</i> and <i>p74</i> transcripts levels decreased significantly. These results indicated that BmNPV <i>p26</i> may be associated with BmNPV replication during the late infection stage.https://www.mdpi.com/2075-4450/12/8/707BmNPV<i>p26</i>expressionsubcellular localizationknockdownreplication |
| spellingShingle | Jun-Qing Ge Zhu-Hong Wang Xi Chen Hua Chen Jian Huang <i>Bombyx mori</i> Nucleopolyhedrovirus <i>p26</i> Is Associated with Viral Late Stage Replication Insects BmNPV <i>p26</i> expression subcellular localization knockdown replication |
| title | <i>Bombyx mori</i> Nucleopolyhedrovirus <i>p26</i> Is Associated with Viral Late Stage Replication |
| title_full | <i>Bombyx mori</i> Nucleopolyhedrovirus <i>p26</i> Is Associated with Viral Late Stage Replication |
| title_fullStr | <i>Bombyx mori</i> Nucleopolyhedrovirus <i>p26</i> Is Associated with Viral Late Stage Replication |
| title_full_unstemmed | <i>Bombyx mori</i> Nucleopolyhedrovirus <i>p26</i> Is Associated with Viral Late Stage Replication |
| title_short | <i>Bombyx mori</i> Nucleopolyhedrovirus <i>p26</i> Is Associated with Viral Late Stage Replication |
| title_sort | i bombyx mori i nucleopolyhedrovirus i p26 i is associated with viral late stage replication |
| topic | BmNPV <i>p26</i> expression subcellular localization knockdown replication |
| url | https://www.mdpi.com/2075-4450/12/8/707 |
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