Intracellular Ca2+ Regulation in Calcium Sensitive Phenotype of Saccharomyces cerevisiae
Intracellular cytosolic Ca2+ concentration accumulation plays an essential information in Saccharomyces cerevisiae i.e. to explain cellular mechanism of Ca2+ sensitive phenotype. Disruption both S. cerevisiae PPase PTP2 and MSG5 genes showed an inhibited growth in the presence of Ca2+. On the other...
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| Format: | Article |
| Language: | English |
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Bogor Agricultural University
2010-03-01
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| Series: | Hayati Journal of Biosciences |
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| Online Access: | http://journal.ipb.ac.id/index.php/hayati/article/view/296/158 |
| _version_ | 1819274368503513088 |
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| author | HERMANSYAH |
| author_facet | HERMANSYAH |
| author_sort | HERMANSYAH |
| collection | DOAJ |
| description | Intracellular cytosolic Ca2+ concentration accumulation plays an essential information in Saccharomyces cerevisiae i.e. to explain cellular mechanism of Ca2+ sensitive phenotype. Disruption both S. cerevisiae PPase PTP2 and MSG5 genes showed an inhibited growth in the presence of Ca2+. On the other hand, by using Luminocounter with apoaequorin system, a method based upon luminescent photoprotein aequorin, intracellular Ca2+ concentration was accumulated as a consequence of calcium sensitive phenotype of S. cerevisiae. This fact indicated that PPase ptp2and msg5 were involved in intracellular Ca2+ transport in addition their already known pathways i.e Mitogen Activated Protein Kinase cell wall integrity pathway, high osmolarity glycerol (HOG) pathway, and pheromone response FUS3 pathway. |
| first_indexed | 2024-12-23T23:07:19Z |
| format | Article |
| id | doaj.art-a2905538e018438eb78e61d2ac160838 |
| institution | Directory Open Access Journal |
| issn | 1978-3019 2086-4094 |
| language | English |
| last_indexed | 2024-12-23T23:07:19Z |
| publishDate | 2010-03-01 |
| publisher | Bogor Agricultural University |
| record_format | Article |
| series | Hayati Journal of Biosciences |
| spelling | doaj.art-a2905538e018438eb78e61d2ac1608382022-12-21T17:26:46ZengBogor Agricultural UniversityHayati Journal of Biosciences1978-30192086-40942010-03-0117114Intracellular Ca2+ Regulation in Calcium Sensitive Phenotype of Saccharomyces cerevisiaeHERMANSYAHIntracellular cytosolic Ca2+ concentration accumulation plays an essential information in Saccharomyces cerevisiae i.e. to explain cellular mechanism of Ca2+ sensitive phenotype. Disruption both S. cerevisiae PPase PTP2 and MSG5 genes showed an inhibited growth in the presence of Ca2+. On the other hand, by using Luminocounter with apoaequorin system, a method based upon luminescent photoprotein aequorin, intracellular Ca2+ concentration was accumulated as a consequence of calcium sensitive phenotype of S. cerevisiae. This fact indicated that PPase ptp2and msg5 were involved in intracellular Ca2+ transport in addition their already known pathways i.e Mitogen Activated Protein Kinase cell wall integrity pathway, high osmolarity glycerol (HOG) pathway, and pheromone response FUS3 pathway.http://journal.ipb.ac.id/index.php/hayati/article/view/296/158Saccharomyces cerevisiaeproteinphosphatasecalcium sensitivitycalcium accumulation |
| spellingShingle | HERMANSYAH Intracellular Ca2+ Regulation in Calcium Sensitive Phenotype of Saccharomyces cerevisiae Hayati Journal of Biosciences Saccharomyces cerevisiae protein phosphatase calcium sensitivity calcium accumulation |
| title | Intracellular Ca2+ Regulation in Calcium Sensitive Phenotype of Saccharomyces cerevisiae |
| title_full | Intracellular Ca2+ Regulation in Calcium Sensitive Phenotype of Saccharomyces cerevisiae |
| title_fullStr | Intracellular Ca2+ Regulation in Calcium Sensitive Phenotype of Saccharomyces cerevisiae |
| title_full_unstemmed | Intracellular Ca2+ Regulation in Calcium Sensitive Phenotype of Saccharomyces cerevisiae |
| title_short | Intracellular Ca2+ Regulation in Calcium Sensitive Phenotype of Saccharomyces cerevisiae |
| title_sort | intracellular ca2 regulation in calcium sensitive phenotype of saccharomyces cerevisiae |
| topic | Saccharomyces cerevisiae protein phosphatase calcium sensitivity calcium accumulation |
| url | http://journal.ipb.ac.id/index.php/hayati/article/view/296/158 |
| work_keys_str_mv | AT hermansyah intracellularca2regulationincalciumsensitivephenotypeofsaccharomycescerevisiae |