Antimicrobial Resistance and Genomic Characterization of <i>Salmonella</i> Infantis from Different Sources
The epidemiology of Salmonella Infantis is complex in terms of its distribution and transmission. The continuous collection and analysis of updated data on the prevalence and antimicrobic resistance are essential. The present work aimed to investigate the antimicrobial resistance and the correlation...
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2023-03-01
|
| Series: | International Journal of Molecular Sciences |
| Subjects: | |
| Online Access: | https://www.mdpi.com/1422-0067/24/6/5492 |
| _version_ | 1797611257492144128 |
|---|---|
| author | Angela Michela Immacolata Montone Anna Cutarelli Maria Francesca Peruzy Immacolata La Tela Roberta Brunetti Maria Gerarda Pirofalo Veronica Folliero Anna Balestrieri Nicoletta Murru Federico Capuano |
| author_facet | Angela Michela Immacolata Montone Anna Cutarelli Maria Francesca Peruzy Immacolata La Tela Roberta Brunetti Maria Gerarda Pirofalo Veronica Folliero Anna Balestrieri Nicoletta Murru Federico Capuano |
| author_sort | Angela Michela Immacolata Montone |
| collection | DOAJ |
| description | The epidemiology of Salmonella Infantis is complex in terms of its distribution and transmission. The continuous collection and analysis of updated data on the prevalence and antimicrobic resistance are essential. The present work aimed to investigate the antimicrobial resistance and the correlation among <i>S.</i> Infantis isolates from different sources through the multiple-locus variable-number of tandem repeat (VNTR) analysis (MLVA). A total of 562 Salmonella strains isolated from 2018 to 2020 from poultry, humans, swine, water buffalo, mussels, cattle, and wild boar were serotyped, and 185 <i>S.</i> Infantis strains (32.92%) were identified. <i>S.</i> Infantis was commonly isolated in poultry and, to a lesser extent, in other sources. The isolates were tested against 12 antimicrobials, and a high prevalence of resistant strains was recorded. <i>S.</i> Infantis showed high resistance against fluoroquinolones, ampicillin, and tetracycline, which are commonly used in human and veterinary medicine. From all <i>S.</i> Infantis isolates, five VNTR loci were amplified. The use of MLVA was not sufficient to understand the complexity of the epidemiological relationships between <i>S.</i> Infantis strains. In conclusion, an alternative methodology to investigate genetic similarities and differences among <i>S.</i> Infantis strains is needed. |
| first_indexed | 2024-03-11T06:26:17Z |
| format | Article |
| id | doaj.art-a2908c41295341c18b10b72e0b1ed602 |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:26:17Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a2908c41295341c18b10b72e0b1ed6022023-11-17T11:34:41ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246549210.3390/ijms24065492Antimicrobial Resistance and Genomic Characterization of <i>Salmonella</i> Infantis from Different SourcesAngela Michela Immacolata Montone0Anna Cutarelli1Maria Francesca Peruzy2Immacolata La Tela3Roberta Brunetti4Maria Gerarda Pirofalo5Veronica Folliero6Anna Balestrieri7Nicoletta Murru8Federico Capuano9Department of Food Inspection, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute 2, 80055 Portici, ItalyDepartment of Food Inspection, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute 2, 80055 Portici, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Delpino 1, 80137 Naples, ItalySalmonella Typing Centre of the Campania Region-Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute 2, 80055 Portici, ItalyEpidemiology and Biostatistics Coordination Department, Experimental Zooprophylactic Institute of Southern, Via Salute 2, 80055 Portici, ItalyComplex Operative Unit of Pathology and Microbiology, Microbiology Sector, University Hospital San Giovanni di Dio e Ruggi d’ Aragona, Largo Città di Ippocrate, 84131 Salerno, ItalyDepartment of Experimental Medicine, University of Campania “Luigi Vanvitelli”, 80138 Naples, ItalySalmonella Typing Centre of the Campania Region-Department of Food Microbiology, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute 2, 80055 Portici, ItalyDepartment of Veterinary Medicine and Animal Production, University of Naples Federico II, Via Delpino 1, 80137 Naples, ItalyDepartment of Food Inspection, Istituto Zooprofilattico Sperimentale del Mezzogiorno, Via Salute 2, 80055 Portici, ItalyThe epidemiology of Salmonella Infantis is complex in terms of its distribution and transmission. The continuous collection and analysis of updated data on the prevalence and antimicrobic resistance are essential. The present work aimed to investigate the antimicrobial resistance and the correlation among <i>S.</i> Infantis isolates from different sources through the multiple-locus variable-number of tandem repeat (VNTR) analysis (MLVA). A total of 562 Salmonella strains isolated from 2018 to 2020 from poultry, humans, swine, water buffalo, mussels, cattle, and wild boar were serotyped, and 185 <i>S.</i> Infantis strains (32.92%) were identified. <i>S.</i> Infantis was commonly isolated in poultry and, to a lesser extent, in other sources. The isolates were tested against 12 antimicrobials, and a high prevalence of resistant strains was recorded. <i>S.</i> Infantis showed high resistance against fluoroquinolones, ampicillin, and tetracycline, which are commonly used in human and veterinary medicine. From all <i>S.</i> Infantis isolates, five VNTR loci were amplified. The use of MLVA was not sufficient to understand the complexity of the epidemiological relationships between <i>S.</i> Infantis strains. In conclusion, an alternative methodology to investigate genetic similarities and differences among <i>S.</i> Infantis strains is needed.https://www.mdpi.com/1422-0067/24/6/5492<i>S</i>. Infantisantimicrobial resistancepoultryMLVA |
| spellingShingle | Angela Michela Immacolata Montone Anna Cutarelli Maria Francesca Peruzy Immacolata La Tela Roberta Brunetti Maria Gerarda Pirofalo Veronica Folliero Anna Balestrieri Nicoletta Murru Federico Capuano Antimicrobial Resistance and Genomic Characterization of <i>Salmonella</i> Infantis from Different Sources International Journal of Molecular Sciences <i>S</i>. Infantis antimicrobial resistance poultry MLVA |
| title | Antimicrobial Resistance and Genomic Characterization of <i>Salmonella</i> Infantis from Different Sources |
| title_full | Antimicrobial Resistance and Genomic Characterization of <i>Salmonella</i> Infantis from Different Sources |
| title_fullStr | Antimicrobial Resistance and Genomic Characterization of <i>Salmonella</i> Infantis from Different Sources |
| title_full_unstemmed | Antimicrobial Resistance and Genomic Characterization of <i>Salmonella</i> Infantis from Different Sources |
| title_short | Antimicrobial Resistance and Genomic Characterization of <i>Salmonella</i> Infantis from Different Sources |
| title_sort | antimicrobial resistance and genomic characterization of i salmonella i infantis from different sources |
| topic | <i>S</i>. Infantis antimicrobial resistance poultry MLVA |
| url | https://www.mdpi.com/1422-0067/24/6/5492 |
| work_keys_str_mv | AT angelamichelaimmacolatamontone antimicrobialresistanceandgenomiccharacterizationofisalmonellaiinfantisfromdifferentsources AT annacutarelli antimicrobialresistanceandgenomiccharacterizationofisalmonellaiinfantisfromdifferentsources AT mariafrancescaperuzy antimicrobialresistanceandgenomiccharacterizationofisalmonellaiinfantisfromdifferentsources AT immacolatalatela antimicrobialresistanceandgenomiccharacterizationofisalmonellaiinfantisfromdifferentsources AT robertabrunetti antimicrobialresistanceandgenomiccharacterizationofisalmonellaiinfantisfromdifferentsources AT mariagerardapirofalo antimicrobialresistanceandgenomiccharacterizationofisalmonellaiinfantisfromdifferentsources AT veronicafolliero antimicrobialresistanceandgenomiccharacterizationofisalmonellaiinfantisfromdifferentsources AT annabalestrieri antimicrobialresistanceandgenomiccharacterizationofisalmonellaiinfantisfromdifferentsources AT nicolettamurru antimicrobialresistanceandgenomiccharacterizationofisalmonellaiinfantisfromdifferentsources AT federicocapuano antimicrobialresistanceandgenomiccharacterizationofisalmonellaiinfantisfromdifferentsources |