Vorinostat enhances chemosensitivity to arsenic trioxide in K562 cell line
Objective. This study aimed to investigate the chemosensitive augmentation effect and mechanism of HDAC inhibitor Vorinostat (SAHA) in combination with arsenic trioxide (ATO) on proliferation and apoptosis of K562 cells.Methods. The CCK-8 assay was used to compare proliferation of the cells. Annexin...
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PeerJ Inc.
2015-05-01
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| Online Access: | https://peerj.com/articles/962.pdf |
| _version_ | 1797418681181929472 |
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| author | Nainong Li Xiaoyan Guan Fang Li Xiaofan Li Yuanzhong Chen |
| author_facet | Nainong Li Xiaoyan Guan Fang Li Xiaofan Li Yuanzhong Chen |
| author_sort | Nainong Li |
| collection | DOAJ |
| description | Objective. This study aimed to investigate the chemosensitive augmentation effect and mechanism of HDAC inhibitor Vorinostat (SAHA) in combination with arsenic trioxide (ATO) on proliferation and apoptosis of K562 cells.Methods. The CCK-8 assay was used to compare proliferation of the cells. Annexin-V and PI staining by flow cytometry and acridine orange/ethidium bromide stains were used to detect and quantify apoptosis. Western blot was used to detect expression of p21, Akt, pAkt, p210, Acetyl-Histone H3, and Acetyl-Histone H4 proteins.Results. SAHA and ATO inhibited proliferation of K562 cells in an additive and time- and dose-dependent manner. SAHA in combination with ATO showed significant apoptosis of K562 cells in comparison to the single drugs alone (p < 0.01). Both SAHA and ATO alone and in combination showed lower levels of p210 expression. SAHA and SAHA and ATO combined treatment showed increased levels of Acetyl-Histone H3 and Acetyl-Histone H4 protein expression. SAHA alone showed increased expression of p21, while ATO alone and in combination with SAHA showed no significant change. SAHA and ATO combined therapy showed lower levels of Akt and pAkt protein expression than SAHA or ATO alone.Conclusion. SAHA and ATO combined treatment inhibited proliferation, induced apoptosis, and showed a chemosensitive augmentation effect on K562 cells. The mechanism might be associated with increasing histone acetylation levels as well as regulating the Akt signaling pathway. |
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| issn | 2167-8359 |
| language | English |
| last_indexed | 2024-03-09T06:36:23Z |
| publishDate | 2015-05-01 |
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| spelling | doaj.art-a291cc055504434b89a5a9260efc20f42023-12-03T10:57:02ZengPeerJ Inc.PeerJ2167-83592015-05-013e96210.7717/peerj.962962Vorinostat enhances chemosensitivity to arsenic trioxide in K562 cell lineNainong Li0Xiaoyan Guan1Fang Li2Xiaofan Li3Yuanzhong Chen4Department of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou, PR ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou, PR ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou, PR ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou, PR ChinaDepartment of Hematology, Fujian Institute of Hematology, Fujian Provincial Key Laboratory on Hematology, Fujian Medical University Union Hospital, Fuzhou, PR ChinaObjective. This study aimed to investigate the chemosensitive augmentation effect and mechanism of HDAC inhibitor Vorinostat (SAHA) in combination with arsenic trioxide (ATO) on proliferation and apoptosis of K562 cells.Methods. The CCK-8 assay was used to compare proliferation of the cells. Annexin-V and PI staining by flow cytometry and acridine orange/ethidium bromide stains were used to detect and quantify apoptosis. Western blot was used to detect expression of p21, Akt, pAkt, p210, Acetyl-Histone H3, and Acetyl-Histone H4 proteins.Results. SAHA and ATO inhibited proliferation of K562 cells in an additive and time- and dose-dependent manner. SAHA in combination with ATO showed significant apoptosis of K562 cells in comparison to the single drugs alone (p < 0.01). Both SAHA and ATO alone and in combination showed lower levels of p210 expression. SAHA and SAHA and ATO combined treatment showed increased levels of Acetyl-Histone H3 and Acetyl-Histone H4 protein expression. SAHA alone showed increased expression of p21, while ATO alone and in combination with SAHA showed no significant change. SAHA and ATO combined therapy showed lower levels of Akt and pAkt protein expression than SAHA or ATO alone.Conclusion. SAHA and ATO combined treatment inhibited proliferation, induced apoptosis, and showed a chemosensitive augmentation effect on K562 cells. The mechanism might be associated with increasing histone acetylation levels as well as regulating the Akt signaling pathway.https://peerj.com/articles/962.pdfHistone deacetylase inhibitorsArsenic trioxideK562 cellApoptosis |
| spellingShingle | Nainong Li Xiaoyan Guan Fang Li Xiaofan Li Yuanzhong Chen Vorinostat enhances chemosensitivity to arsenic trioxide in K562 cell line PeerJ Histone deacetylase inhibitors Arsenic trioxide K562 cell Apoptosis |
| title | Vorinostat enhances chemosensitivity to arsenic trioxide in K562 cell line |
| title_full | Vorinostat enhances chemosensitivity to arsenic trioxide in K562 cell line |
| title_fullStr | Vorinostat enhances chemosensitivity to arsenic trioxide in K562 cell line |
| title_full_unstemmed | Vorinostat enhances chemosensitivity to arsenic trioxide in K562 cell line |
| title_short | Vorinostat enhances chemosensitivity to arsenic trioxide in K562 cell line |
| title_sort | vorinostat enhances chemosensitivity to arsenic trioxide in k562 cell line |
| topic | Histone deacetylase inhibitors Arsenic trioxide K562 cell Apoptosis |
| url | https://peerj.com/articles/962.pdf |
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