Melanogenesis Inhibitory and Antioxidant Effects of Camellia oleifera Seed Oil
Purpose: The study aimed to characterize the fatty acid profile of Camellia oleifera (tea) seed oil and evaluate for cytotoxicity and activities on melanogenesis and antioxidant activity assays in order to utilize as the functional oil. Methods: The fatty acid profile of oil was analyzed by gas chro...
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| Format: | Article |
| Language: | English |
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Tabriz University of Medical Sciences
2017-09-01
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| Series: | Advanced Pharmaceutical Bulletin |
| Subjects: | |
| Online Access: | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-473.pdf |
| _version_ | 1818526061513670656 |
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| author | Puxvadee Chaikul Tawanun Sripisut Setinee Chanpirom Kanchanapa Sathirachawan Naphatsorn Ditthawutthikul |
| author_facet | Puxvadee Chaikul Tawanun Sripisut Setinee Chanpirom Kanchanapa Sathirachawan Naphatsorn Ditthawutthikul |
| author_sort | Puxvadee Chaikul |
| collection | DOAJ |
| description | Purpose: The study aimed to characterize the
fatty acid profile of Camellia oleifera (tea) seed oil and
evaluate for cytotoxicity and activities on melanogenesis and antioxidant
activity assays in order to utilize as the functional oil.
Methods: The fatty acid profile of oil was analyzed by gas chromatography/mass
spectrometry (GC/MS). The cytotoxicity was performed by sulforhodamine B (SRB)
assay in B16-F10 melanoma cells and 3T3-L1 cells. The melanogenesis assay,
including melanin content and activities of tyrosinase and tyrosinase-related
protein-2 (TRP-2), and antioxidant activity were evaluated.
Results: Three major fatty acids of oil were
oleic acid (87.93±0.19%), stearic (5.14±0.06%) and palmitic (5.08±0.12%) acids.
The non-cytotoxicity of 5% tea seed oil
demonstrated the cell viabilities of 94.59±3.41% in B16-F10 melanoma
cells and 97.57±1.62% in 3T3-L1
cells. Tea seed oil exhibited the inhibitory activity on melanogenesis assay
via inhibition of tyrosinase and TRP-2 activities. The antioxidant activity of
3% tea seed oil appeared the cellular protection with cell viability of 90.38±7.77%.
Conclusion: The results of study have shown the potential utilization of tea seed oil
as the functional oil in several products, including health, food and cosmetic products. |
| first_indexed | 2024-12-11T06:17:48Z |
| format | Article |
| id | doaj.art-a292e3fa3e4243b5a482d420353fb3d4 |
| institution | Directory Open Access Journal |
| issn | 2228-5881 2251-7308 |
| language | English |
| last_indexed | 2024-12-11T06:17:48Z |
| publishDate | 2017-09-01 |
| publisher | Tabriz University of Medical Sciences |
| record_format | Article |
| series | Advanced Pharmaceutical Bulletin |
| spelling | doaj.art-a292e3fa3e4243b5a482d420353fb3d42022-12-22T01:17:56ZengTabriz University of Medical SciencesAdvanced Pharmaceutical Bulletin2228-58812251-73082017-09-017347347710.15171/apb.2017.057APB_19444_20170616174328Melanogenesis Inhibitory and Antioxidant Effects of Camellia oleifera Seed OilPuxvadee Chaikul0Tawanun Sripisut1Setinee Chanpirom2Kanchanapa Sathirachawan3Naphatsorn Ditthawutthikul4School of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.School of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.School of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.School of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.School of Cosmetic Science, Mae Fah Luang University, Chiang Rai 57100, Thailand.Purpose: The study aimed to characterize the fatty acid profile of Camellia oleifera (tea) seed oil and evaluate for cytotoxicity and activities on melanogenesis and antioxidant activity assays in order to utilize as the functional oil. Methods: The fatty acid profile of oil was analyzed by gas chromatography/mass spectrometry (GC/MS). The cytotoxicity was performed by sulforhodamine B (SRB) assay in B16-F10 melanoma cells and 3T3-L1 cells. The melanogenesis assay, including melanin content and activities of tyrosinase and tyrosinase-related protein-2 (TRP-2), and antioxidant activity were evaluated. Results: Three major fatty acids of oil were oleic acid (87.93±0.19%), stearic (5.14±0.06%) and palmitic (5.08±0.12%) acids. The non-cytotoxicity of 5% tea seed oil demonstrated the cell viabilities of 94.59±3.41% in B16-F10 melanoma cells and 97.57±1.62% in 3T3-L1 cells. Tea seed oil exhibited the inhibitory activity on melanogenesis assay via inhibition of tyrosinase and TRP-2 activities. The antioxidant activity of 3% tea seed oil appeared the cellular protection with cell viability of 90.38±7.77%. Conclusion: The results of study have shown the potential utilization of tea seed oil as the functional oil in several products, including health, food and cosmetic products.http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-473.pdfTea seed oilCytotoxicityMelanogenesisAntioxidant Cosmeticcosmetic |
| spellingShingle | Puxvadee Chaikul Tawanun Sripisut Setinee Chanpirom Kanchanapa Sathirachawan Naphatsorn Ditthawutthikul Melanogenesis Inhibitory and Antioxidant Effects of Camellia oleifera Seed Oil Advanced Pharmaceutical Bulletin Tea seed oil Cytotoxicity Melanogenesis Antioxidant Cosmetic cosmetic |
| title | Melanogenesis Inhibitory and Antioxidant Effects of
Camellia oleifera Seed Oil |
| title_full | Melanogenesis Inhibitory and Antioxidant Effects of
Camellia oleifera Seed Oil |
| title_fullStr | Melanogenesis Inhibitory and Antioxidant Effects of
Camellia oleifera Seed Oil |
| title_full_unstemmed | Melanogenesis Inhibitory and Antioxidant Effects of
Camellia oleifera Seed Oil |
| title_short | Melanogenesis Inhibitory and Antioxidant Effects of
Camellia oleifera Seed Oil |
| title_sort | melanogenesis inhibitory and antioxidant effects of camellia oleifera seed oil |
| topic | Tea seed oil Cytotoxicity Melanogenesis Antioxidant Cosmetic cosmetic |
| url | http://journals.tbzmed.ac.ir/APB/Manuscript/APB-7-473.pdf |
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