Genome-wide characterization and phylogenetic and expression analyses of the caleosin gene family in soybean, common bean and barrel medic
Caleosin are a class of calcium-binding proteins embedded in the phospholipid monolayer of lipid droplets. In addition to maintaining thestructure of lipid droplets, caleosin proteins areinvolved in dormancy and lipid signaling, and areassociatedwith the stress response via their histidine-...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Belgrade, University of Novi Sad
2016-01-01
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| Series: | Archives of Biological Sciences |
| Subjects: | |
| Online Access: | http://www.doiserbia.nb.rs/img/doi/0354-4664/2016/0354-46641600048S.pdf |
| Summary: | Caleosin are a class of calcium-binding proteins embedded in the phospholipid
monolayer of lipid droplets. In addition to maintaining thestructure of lipid
droplets, caleosin proteins areinvolved in dormancy and lipid signaling, and
areassociatedwith the stress response via their histidine-dependent
peroxygenase activity. To date, caleosins have been studied in Arabidopsis
thaliana. However, little is known about these genes in legumes,including the
most cultivated oilseed crop, soybean. In this paper,20 caleosin genes in
soybean, common bean and barrel medic werestudied. Among these, 13 caleosin
genes, including 3 in Glycine max, 5 in Phaseolus vulgarisand 5 in Medicago
truncatula, are identified for the first time. The structures,
characteristics and evolution of the 20 caleosin proteins are analyzed.
Expansion patterns show that tandem duplication was the main reason for the
caleosin family expansion in the legume. Expression profiles indicate that
L-caleosin in soybean and common bean are more important than H-caleosin,
which is just the opposite in Arabidopsis thaliana. GmaCLO2, PvuCLO1,
PvuCLO3and MtrCLO3may play important roles, while GmaCLO6, GmaCLO10and
MtrCLO4may lose their function in the examined tissues. In addition,
according to the results of cis-element analyses, we propose potential
functions for the more important caleosin genes in leguminous plants. Our
work provides helpful information for further evolution and function analyses
of the caleosin gene family in soybean, common bean and barrel medic. |
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| ISSN: | 0354-4664 1821-4339 |