Isolation, molecular identification, and antifungal susceptibility profiles of vaginal isolates of Candida species
Background and Objectives: Vulvovaginal candidiasis is a common fungal infection among women during reproductive ages. Although, Candida albicans is accounted as the main etiologic agent of vaginitis, non-albicans species have arisen during last years. Resistant to antifungal drugs especially, fluco...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2017-03-01
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| Series: | Iranian Journal of Microbiology |
| Subjects: | |
| Online Access: | https://ijm.tums.ac.ir/index.php/ijm/article/view/1162 |
| _version_ | 1818383285922824192 |
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| author | Ali Rezaei-Matehkolaei Shokoofe Shafiei Ali Zarei Mahmoudabadi |
| author_facet | Ali Rezaei-Matehkolaei Shokoofe Shafiei Ali Zarei Mahmoudabadi |
| author_sort | Ali Rezaei-Matehkolaei |
| collection | DOAJ |
| description | Background and Objectives: Vulvovaginal candidiasis is a common fungal infection among women during reproductive ages. Although, Candida albicans is accounted as the main etiologic agent of vaginitis, non-albicans species have arisen during last years. Resistant to antifungal drugs especially, fluconazole has been more reported by researchers from around the World. The aims of this study were to determine the prevalence of vulvovaginal candidiasis among suspected patients with vaginitis, the frequency of Candida species, and the susceptibility profiles of isolates to caspofungin, fluconazole and clotrimazole.
Materials and Methods: One hundred and twenty suspected women with vaginitis were examined by specialist physician and sampled using moisture swabs. Swabs were inoculated on CHROMagar Candida plates, incubated at 35ºC and detected all isolated Candida species using morphological, microcopy and molecular methods. The antifungal susceptibility tests with caspofungin, fluconazole and clotrimazole were applied using microdilution and Resazurin dye methods against all isolated yeasts.
Results: The cultures were positive for 34(28.3%) samples and three Candida species including; C. albicans (88.2%), C. glabrata (8.8%) and C. kefyr (2.9%). Our study shows that only one isolate of C. albicans was resistant to caspofungin at the concentration of 2 μg/ml after 24h incubation that increased to 2 isolates after 48h incubation. All isolates were sensitive to fluconazole at the MIC ranges of 1-0.25 μg/ml, while 88.2% of them were inhibited at 0.25 μg/mL of clotrimazole. Candida albicans remains the most common agent of fungal vaginitis.
Conclusion: Although all of Candida isolates were susceptible to fluconazole in vitro, it should be used with caution for empirical therapy due to more resistant rates in clinic. In addition, due to valuable sensitivity of all tested strains to caspofungin, it potentially can be presented as the first line therapy for Candida vaginitis. |
| first_indexed | 2024-12-14T03:03:57Z |
| format | Article |
| id | doaj.art-a294af528f824526918ed113f6206f39 |
| institution | Directory Open Access Journal |
| issn | 2008-3289 2008-4447 |
| language | English |
| last_indexed | 2024-12-14T03:03:57Z |
| publishDate | 2017-03-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Microbiology |
| spelling | doaj.art-a294af528f824526918ed113f6206f392022-12-21T23:19:27ZengTehran University of Medical SciencesIranian Journal of Microbiology2008-32892008-44472017-03-0186Isolation, molecular identification, and antifungal susceptibility profiles of vaginal isolates of Candida speciesAli Rezaei-Matehkolaei0Shokoofe Shafiei1Ali Zarei Mahmoudabadi2Infectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran AND Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranDepartment of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranInfectious and Tropical Diseases Research Center, Health Research Institute, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, Iran AND Department of Medical Mycology, School of Medicine, Ahvaz Jundishapur University of Medical Sciences, Ahvaz, IranBackground and Objectives: Vulvovaginal candidiasis is a common fungal infection among women during reproductive ages. Although, Candida albicans is accounted as the main etiologic agent of vaginitis, non-albicans species have arisen during last years. Resistant to antifungal drugs especially, fluconazole has been more reported by researchers from around the World. The aims of this study were to determine the prevalence of vulvovaginal candidiasis among suspected patients with vaginitis, the frequency of Candida species, and the susceptibility profiles of isolates to caspofungin, fluconazole and clotrimazole. Materials and Methods: One hundred and twenty suspected women with vaginitis were examined by specialist physician and sampled using moisture swabs. Swabs were inoculated on CHROMagar Candida plates, incubated at 35ºC and detected all isolated Candida species using morphological, microcopy and molecular methods. The antifungal susceptibility tests with caspofungin, fluconazole and clotrimazole were applied using microdilution and Resazurin dye methods against all isolated yeasts. Results: The cultures were positive for 34(28.3%) samples and three Candida species including; C. albicans (88.2%), C. glabrata (8.8%) and C. kefyr (2.9%). Our study shows that only one isolate of C. albicans was resistant to caspofungin at the concentration of 2 μg/ml after 24h incubation that increased to 2 isolates after 48h incubation. All isolates were sensitive to fluconazole at the MIC ranges of 1-0.25 μg/ml, while 88.2% of them were inhibited at 0.25 μg/mL of clotrimazole. Candida albicans remains the most common agent of fungal vaginitis. Conclusion: Although all of Candida isolates were susceptible to fluconazole in vitro, it should be used with caution for empirical therapy due to more resistant rates in clinic. In addition, due to valuable sensitivity of all tested strains to caspofungin, it potentially can be presented as the first line therapy for Candida vaginitis.https://ijm.tums.ac.ir/index.php/ijm/article/view/1162CaspofunginFluconazoleClotrimazoleCandida vaginitisCandida albicans |
| spellingShingle | Ali Rezaei-Matehkolaei Shokoofe Shafiei Ali Zarei Mahmoudabadi Isolation, molecular identification, and antifungal susceptibility profiles of vaginal isolates of Candida species Iranian Journal of Microbiology Caspofungin Fluconazole Clotrimazole Candida vaginitis Candida albicans |
| title | Isolation, molecular identification, and antifungal susceptibility profiles of vaginal isolates of Candida species |
| title_full | Isolation, molecular identification, and antifungal susceptibility profiles of vaginal isolates of Candida species |
| title_fullStr | Isolation, molecular identification, and antifungal susceptibility profiles of vaginal isolates of Candida species |
| title_full_unstemmed | Isolation, molecular identification, and antifungal susceptibility profiles of vaginal isolates of Candida species |
| title_short | Isolation, molecular identification, and antifungal susceptibility profiles of vaginal isolates of Candida species |
| title_sort | isolation molecular identification and antifungal susceptibility profiles of vaginal isolates of candida species |
| topic | Caspofungin Fluconazole Clotrimazole Candida vaginitis Candida albicans |
| url | https://ijm.tums.ac.ir/index.php/ijm/article/view/1162 |
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