Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms
Abstract Background Angiogenesis can occur under pathological conditions when stimuli such as inflammation, vascular obstruction or hypoxia exist. These stimuli are present in cardiopulmonary dirofilariosis (Dirofilaria immitis). The aim of this study was to analyze the capacity of D. immitis antige...
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| Language: | English |
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BMC
2019-06-01
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| Series: | Parasites & Vectors |
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| Online Access: | http://link.springer.com/article/10.1186/s13071-019-3570-0 |
| _version_ | 1818244893477175296 |
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| author | Tatiana Zueva Rodrigo Morchón Elena Carretón José Alberto Montoya-Alonso Alexis Santana María Dolores Bargues Santiago Mas-Coma Alicia Rodríguez-Barbero Fernando Simón |
| author_facet | Tatiana Zueva Rodrigo Morchón Elena Carretón José Alberto Montoya-Alonso Alexis Santana María Dolores Bargues Santiago Mas-Coma Alicia Rodríguez-Barbero Fernando Simón |
| author_sort | Tatiana Zueva |
| collection | DOAJ |
| description | Abstract Background Angiogenesis can occur under pathological conditions when stimuli such as inflammation, vascular obstruction or hypoxia exist. These stimuli are present in cardiopulmonary dirofilariosis (Dirofilaria immitis). The aim of this study was to analyze the capacity of D. immitis antigens to modify the expression of angiogenic factors and trigger the formation of pseudocapillaries (tube-like structures) in an in vitro model of endothelial cells. Methods The expression of VEGF-A, sFlt, mEndoglin and sEndoglin in cultures of canine microvascular endothelial cells stimulated with extract of adult worms of D. immitis obtained from an untreated dog (DiSA) and from a dog treated for 15 days with doxycycline (tDiSA), was determined by using commercial kits. The capacity of pseudocapillary formation was evaluated analyzing cell connections and cell groups in Matrigel cell cultures stimulated with DiSA and tDiSA. In both cases non-stimulated cultures were used as controls. Results First, we demonstrated that worms obtained from the dog treated with doxycycline showed a significantly lower amount of Wolbachia (less than 60%) than worms removed from the untreated dog. Only DiSA was able to significantly increase the expression of the proangiogenic factor VEGF-A in the endotelial cells cultures. None of the D. immitis extracts modified the expression of sFlt. tDiSA extract was able to modify the expression of the endoglins, significantly decreasing the expression of the pro-angiogenic mEndoglin and increasing the anti-angiogenic sEndoglin. The formation of pseudocapillaries was negatively influenced by tDiSA, which reduced the organization and number of cellular connections. Conclusions The ability of antigens from adult D. immitis worms to modify the expression of pro and anti-angiogenic factors in endotelial cell cultures was demonstrated, as well as the trend to form pseudocapillaries in vitro. The capacity of stimulation may be linked to the amount of Wolbachia present in the antigenic extracts. |
| first_indexed | 2024-12-12T14:24:15Z |
| format | Article |
| id | doaj.art-a296872fdeca441c8054e66f02eaaf21 |
| institution | Directory Open Access Journal |
| issn | 1756-3305 |
| language | English |
| last_indexed | 2024-12-12T14:24:15Z |
| publishDate | 2019-06-01 |
| publisher | BMC |
| record_format | Article |
| series | Parasites & Vectors |
| spelling | doaj.art-a296872fdeca441c8054e66f02eaaf212022-12-22T00:21:43ZengBMCParasites & Vectors1756-33052019-06-011211710.1186/s13071-019-3570-0Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult wormsTatiana Zueva0Rodrigo Morchón1Elena Carretón2José Alberto Montoya-Alonso3Alexis Santana4María Dolores Bargues5Santiago Mas-Coma6Alicia Rodríguez-Barbero7Fernando Simón8Group of Animal and Human Dirofilariosis, Parasitology Area, Faculty of Pharmacy, University of SalamancaGroup of Animal and Human Dirofilariosis, Parasitology Area, Faculty of Pharmacy, University of SalamancaFaculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran CanariaFaculty of Veterinary Medicine, Research Institute of Biomedical and Health Sciences (IUIBS), University of Las Palmas de Gran CanariaAlbea VeterinariosDepartment of Parasitology, Faculty of Pharmacy, University of ValenciaDepartment of Parasitology, Faculty of Pharmacy, University of ValenciaDepartment of Physiology and Pharmacology, Biomedical Research Institute of Salamanca (IBSAL), University of SalamancaGroup of Animal and Human Dirofilariosis, Parasitology Area, Faculty of Pharmacy, University of SalamancaAbstract Background Angiogenesis can occur under pathological conditions when stimuli such as inflammation, vascular obstruction or hypoxia exist. These stimuli are present in cardiopulmonary dirofilariosis (Dirofilaria immitis). The aim of this study was to analyze the capacity of D. immitis antigens to modify the expression of angiogenic factors and trigger the formation of pseudocapillaries (tube-like structures) in an in vitro model of endothelial cells. Methods The expression of VEGF-A, sFlt, mEndoglin and sEndoglin in cultures of canine microvascular endothelial cells stimulated with extract of adult worms of D. immitis obtained from an untreated dog (DiSA) and from a dog treated for 15 days with doxycycline (tDiSA), was determined by using commercial kits. The capacity of pseudocapillary formation was evaluated analyzing cell connections and cell groups in Matrigel cell cultures stimulated with DiSA and tDiSA. In both cases non-stimulated cultures were used as controls. Results First, we demonstrated that worms obtained from the dog treated with doxycycline showed a significantly lower amount of Wolbachia (less than 60%) than worms removed from the untreated dog. Only DiSA was able to significantly increase the expression of the proangiogenic factor VEGF-A in the endotelial cells cultures. None of the D. immitis extracts modified the expression of sFlt. tDiSA extract was able to modify the expression of the endoglins, significantly decreasing the expression of the pro-angiogenic mEndoglin and increasing the anti-angiogenic sEndoglin. The formation of pseudocapillaries was negatively influenced by tDiSA, which reduced the organization and number of cellular connections. Conclusions The ability of antigens from adult D. immitis worms to modify the expression of pro and anti-angiogenic factors in endotelial cell cultures was demonstrated, as well as the trend to form pseudocapillaries in vitro. The capacity of stimulation may be linked to the amount of Wolbachia present in the antigenic extracts.http://link.springer.com/article/10.1186/s13071-019-3570-0Angiogenic factorsPseudocapillaries formationDirofilaria immitis antigenic extractsWolbachia amountCanine microvascular endothelial cells |
| spellingShingle | Tatiana Zueva Rodrigo Morchón Elena Carretón José Alberto Montoya-Alonso Alexis Santana María Dolores Bargues Santiago Mas-Coma Alicia Rodríguez-Barbero Fernando Simón Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms Parasites & Vectors Angiogenic factors Pseudocapillaries formation Dirofilaria immitis antigenic extracts Wolbachia amount Canine microvascular endothelial cells |
| title | Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms |
| title_full | Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms |
| title_fullStr | Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms |
| title_full_unstemmed | Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms |
| title_short | Angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from Dirofilaria immitis adult worms |
| title_sort | angiogenic response in an in vitro model of dog microvascular endothelial cells stimulated with antigenic extracts from dirofilaria immitis adult worms |
| topic | Angiogenic factors Pseudocapillaries formation Dirofilaria immitis antigenic extracts Wolbachia amount Canine microvascular endothelial cells |
| url | http://link.springer.com/article/10.1186/s13071-019-3570-0 |
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