Neuroprotective and antioxidant role of Phoenix dactylifera in permanent bilateral common carotid occlusion in rats
Objective: To investigate neuroprotective and antioxidant effect of Phoenix dactylifera (P. dactylifera) (PD) fruits. Methods: Methanolic extract of P. dactylifera fruits (MEPD) at doses of 30, 100 and 300 mg/kg was studied against permanent BCCAO (long-term hypoperfusion) in rats. Chronic occlusion...
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2014-01-01
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| Series: | Journal of Acute Disease |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2221618914600263 |
| _version_ | 1828266367821807616 |
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| author | Rohini R. Pujari Neeraj S. Vyawahare Prasad A. Thakurdesai |
| author_facet | Rohini R. Pujari Neeraj S. Vyawahare Prasad A. Thakurdesai |
| author_sort | Rohini R. Pujari |
| collection | DOAJ |
| description | Objective: To investigate neuroprotective and antioxidant effect of Phoenix dactylifera (P. dactylifera) (PD) fruits.
Methods: Methanolic extract of P. dactylifera fruits (MEPD) at doses of 30, 100 and 300 mg/kg was studied against permanent BCCAO (long-term hypoperfusion) in rats. Chronic occlusion of bilateral common carotid arteries (BCCA) caused significant elevation in malondialdehyde levels due to increased lipid peroxidation as well as decrease in levels of other biochemical enzymes i.e. glutathione, glutathione peroxidase, glutathione reductase, glutathione-S-transferse, catalase and superoxide dismutase.
Results: Post occlusion treatment for 15 d with 100 and 300 mg/kg doses of MEPD significantly reduced the enhanced malondialdehyde levels and reversed the alterations in the declined levels of antioxidant enzymes in brain homogenates of hypoperfused rats. Long-term cerebral hypoperfusion in rats caused a propensity towards anxiety and restlessness (open field paradigm) accompanied by deficits of spatial learning and memory (Morris water maze testing). Additionally, histopathological observations in hypoperfused brains revealed reactive changes like shrinkage and necrosis of neurons. 100 and 300 mg/kg doses of MEPD significantly alleviated these alterations.
Conclusions: These results confirmed the protective role of P. dactylifera in ischemia hypoperfusion and thereby it's beneficial role in cerebrovascular insufficiency states and related complications. |
| first_indexed | 2024-04-13T04:51:45Z |
| format | Article |
| id | doaj.art-a297131a238b49248b4543bc9b736e5c |
| institution | Directory Open Access Journal |
| issn | 2221-6189 |
| language | English |
| last_indexed | 2024-04-13T04:51:45Z |
| publishDate | 2014-01-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Acute Disease |
| spelling | doaj.art-a297131a238b49248b4543bc9b736e5c2022-12-22T03:01:39ZengWolters Kluwer Medknow PublicationsJournal of Acute Disease2221-61892014-01-013210411410.1016/S2221-6189(14)60026-3Neuroprotective and antioxidant role of Phoenix dactylifera in permanent bilateral common carotid occlusion in ratsRohini R. Pujari0Neeraj S. Vyawahare1Prasad A. Thakurdesai2Department of Pharmacology, Modern College of Pharmacy (Ladies), Moshi, Pune-412105, Maharashtra, IndiaDepartment of Pharmacology, Padmashree Dr. D Y Patil College of Pharmacy, Sector 29, Pradhikaran, Akurdi, Pune – 411 044, Maharashtra, IndiaGM-Scientific affairs, Indus Biotech Private Limited, 1, Rahul residency, Plot No. 5-6, Off Salunke Vihar Road, Kondhva, Pune-411048, Maharashtra, IndiaObjective: To investigate neuroprotective and antioxidant effect of Phoenix dactylifera (P. dactylifera) (PD) fruits. Methods: Methanolic extract of P. dactylifera fruits (MEPD) at doses of 30, 100 and 300 mg/kg was studied against permanent BCCAO (long-term hypoperfusion) in rats. Chronic occlusion of bilateral common carotid arteries (BCCA) caused significant elevation in malondialdehyde levels due to increased lipid peroxidation as well as decrease in levels of other biochemical enzymes i.e. glutathione, glutathione peroxidase, glutathione reductase, glutathione-S-transferse, catalase and superoxide dismutase. Results: Post occlusion treatment for 15 d with 100 and 300 mg/kg doses of MEPD significantly reduced the enhanced malondialdehyde levels and reversed the alterations in the declined levels of antioxidant enzymes in brain homogenates of hypoperfused rats. Long-term cerebral hypoperfusion in rats caused a propensity towards anxiety and restlessness (open field paradigm) accompanied by deficits of spatial learning and memory (Morris water maze testing). Additionally, histopathological observations in hypoperfused brains revealed reactive changes like shrinkage and necrosis of neurons. 100 and 300 mg/kg doses of MEPD significantly alleviated these alterations. Conclusions: These results confirmed the protective role of P. dactylifera in ischemia hypoperfusion and thereby it's beneficial role in cerebrovascular insufficiency states and related complications.http://www.sciencedirect.com/science/article/pii/S2221618914600263Phoenix dactyliferaChronic cerebral hypoperfusionVascular dementiaPermanent bilateralCommon carotid artery occlusionOxidative stress |
| spellingShingle | Rohini R. Pujari Neeraj S. Vyawahare Prasad A. Thakurdesai Neuroprotective and antioxidant role of Phoenix dactylifera in permanent bilateral common carotid occlusion in rats Journal of Acute Disease Phoenix dactylifera Chronic cerebral hypoperfusion Vascular dementia Permanent bilateral Common carotid artery occlusion Oxidative stress |
| title | Neuroprotective and antioxidant role of Phoenix dactylifera in permanent bilateral common carotid occlusion in rats |
| title_full | Neuroprotective and antioxidant role of Phoenix dactylifera in permanent bilateral common carotid occlusion in rats |
| title_fullStr | Neuroprotective and antioxidant role of Phoenix dactylifera in permanent bilateral common carotid occlusion in rats |
| title_full_unstemmed | Neuroprotective and antioxidant role of Phoenix dactylifera in permanent bilateral common carotid occlusion in rats |
| title_short | Neuroprotective and antioxidant role of Phoenix dactylifera in permanent bilateral common carotid occlusion in rats |
| title_sort | neuroprotective and antioxidant role of phoenix dactylifera in permanent bilateral common carotid occlusion in rats |
| topic | Phoenix dactylifera Chronic cerebral hypoperfusion Vascular dementia Permanent bilateral Common carotid artery occlusion Oxidative stress |
| url | http://www.sciencedirect.com/science/article/pii/S2221618914600263 |
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