| Summary: | To study the effect of non-ionic contrast media on anticoagulated and non-anticoagulated human whole blood samples, calorimetric measurements were performed. The anticoagulated plasma showed the greatest fall in the total <i>ΔH</i> after Iodixanol treatment. The plasma-free erythrocytes revealed a pronounced shift in the <i>T<sub>max</sub></i> and a decrease in the <i>ΔH</i> of hemoglobin and transferrin. The total <i>ΔH</i> of Iodixanol treatment showed the highest decline, while Iomeprol and Iobitridol had fewer adverse effects. Similarly, the non-anticoagulated samples revealed a decrease both in the <i>T<sub>max</sub></i> and the <i>ΔH</i> of albumin and immunoglobulin-specific transitions. The total <i>ΔH</i> showed that Iodixanol had more influence on the serum. The serum-free erythrocyte samples resulted in a significant drop in the <i>T<sub>max</sub></i> of erythrocyte and transferrin (~5–6 °C). The <i>ΔH</i> of deconvolved hemoglobin and transferrin decreased considerably; however, the <i>ΔH</i> of albumin increased. Surprisingly, compared to Iomeprol and Iobitridol treatments, the total <i>ΔH</i> of Iodixanol was less pronounced in the non-anticoagulated erythrocyte samples. In sum, each non-ionic contrast medium affected the thermal stability of anticoagulated and non-anticoagulated erythrocyte proteins. Interestingly, Iodixanol treatment caused more significant effects. These findings suggest that conformational changes in blood components can occur, which can potentially lead to the increased prevalence of cardiovascular dysfunctions and blood clotting.
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