Deconvolution Analysis of the Non-Ionic Iomeprol, Iobitridol and Iodixanol Contrast Media-Treated Human Whole Blood Thermograms: A Comparative Study
To study the effect of non-ionic contrast media on anticoagulated and non-anticoagulated human whole blood samples, calorimetric measurements were performed. The anticoagulated plasma showed the greatest fall in the total <i>ΔH</i> after Iodixanol treatment. The plasma-free erythrocytes...
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MDPI AG
2023-07-01
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| Series: | Diagnostics |
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| Online Access: | https://www.mdpi.com/2075-4418/13/15/2523 |
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| author | Elek Telek Zoltán Ujfalusi Miklós Nyitrai Péter Bogner András Lukács Tímea Németh Gabriella Hild Gábor Hild |
| author_facet | Elek Telek Zoltán Ujfalusi Miklós Nyitrai Péter Bogner András Lukács Tímea Németh Gabriella Hild Gábor Hild |
| author_sort | Elek Telek |
| collection | DOAJ |
| description | To study the effect of non-ionic contrast media on anticoagulated and non-anticoagulated human whole blood samples, calorimetric measurements were performed. The anticoagulated plasma showed the greatest fall in the total <i>ΔH</i> after Iodixanol treatment. The plasma-free erythrocytes revealed a pronounced shift in the <i>T<sub>max</sub></i> and a decrease in the <i>ΔH</i> of hemoglobin and transferrin. The total <i>ΔH</i> of Iodixanol treatment showed the highest decline, while Iomeprol and Iobitridol had fewer adverse effects. Similarly, the non-anticoagulated samples revealed a decrease both in the <i>T<sub>max</sub></i> and the <i>ΔH</i> of albumin and immunoglobulin-specific transitions. The total <i>ΔH</i> showed that Iodixanol had more influence on the serum. The serum-free erythrocyte samples resulted in a significant drop in the <i>T<sub>max</sub></i> of erythrocyte and transferrin (~5–6 °C). The <i>ΔH</i> of deconvolved hemoglobin and transferrin decreased considerably; however, the <i>ΔH</i> of albumin increased. Surprisingly, compared to Iomeprol and Iobitridol treatments, the total <i>ΔH</i> of Iodixanol was less pronounced in the non-anticoagulated erythrocyte samples. In sum, each non-ionic contrast medium affected the thermal stability of anticoagulated and non-anticoagulated erythrocyte proteins. Interestingly, Iodixanol treatment caused more significant effects. These findings suggest that conformational changes in blood components can occur, which can potentially lead to the increased prevalence of cardiovascular dysfunctions and blood clotting. |
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| institution | Directory Open Access Journal |
| issn | 2075-4418 |
| language | English |
| last_indexed | 2024-03-11T00:29:38Z |
| publishDate | 2023-07-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Diagnostics |
| spelling | doaj.art-a298b6e399844cf3a27d6da65404ef132023-11-18T22:46:39ZengMDPI AGDiagnostics2075-44182023-07-011315252310.3390/diagnostics13152523Deconvolution Analysis of the Non-Ionic Iomeprol, Iobitridol and Iodixanol Contrast Media-Treated Human Whole Blood Thermograms: A Comparative StudyElek Telek0Zoltán Ujfalusi1Miklós Nyitrai2Péter Bogner3András Lukács4Tímea Németh5Gabriella Hild6Gábor Hild7Department of Biophysics, Medical School, University of Pécs, Szigeti Str. 12, H-7624 Pécs, HungaryDepartment of Biophysics, Medical School, University of Pécs, Szigeti Str. 12, H-7624 Pécs, HungaryDepartment of Biophysics, Medical School, University of Pécs, Szigeti Str. 12, H-7624 Pécs, HungaryDepartment of Medical Imaging, Clinical Centre, University of Pécs, Ifjúság Str. 13, H-7624 Pécs, HungaryDepartment of Biophysics, Medical School, University of Pécs, Szigeti Str. 12, H-7624 Pécs, HungaryLanguages for Biomedical Purposes and Communication, Medical School, University of Pécs, Szigeti Str. 12, H-7624 Pécs, HungaryLanguages for Biomedical Purposes and Communication, Medical School, University of Pécs, Szigeti Str. 12, H-7624 Pécs, HungaryDepartment of Biophysics, Medical School, University of Pécs, Szigeti Str. 12, H-7624 Pécs, HungaryTo study the effect of non-ionic contrast media on anticoagulated and non-anticoagulated human whole blood samples, calorimetric measurements were performed. The anticoagulated plasma showed the greatest fall in the total <i>ΔH</i> after Iodixanol treatment. The plasma-free erythrocytes revealed a pronounced shift in the <i>T<sub>max</sub></i> and a decrease in the <i>ΔH</i> of hemoglobin and transferrin. The total <i>ΔH</i> of Iodixanol treatment showed the highest decline, while Iomeprol and Iobitridol had fewer adverse effects. Similarly, the non-anticoagulated samples revealed a decrease both in the <i>T<sub>max</sub></i> and the <i>ΔH</i> of albumin and immunoglobulin-specific transitions. The total <i>ΔH</i> showed that Iodixanol had more influence on the serum. The serum-free erythrocyte samples resulted in a significant drop in the <i>T<sub>max</sub></i> of erythrocyte and transferrin (~5–6 °C). The <i>ΔH</i> of deconvolved hemoglobin and transferrin decreased considerably; however, the <i>ΔH</i> of albumin increased. Surprisingly, compared to Iomeprol and Iobitridol treatments, the total <i>ΔH</i> of Iodixanol was less pronounced in the non-anticoagulated erythrocyte samples. In sum, each non-ionic contrast medium affected the thermal stability of anticoagulated and non-anticoagulated erythrocyte proteins. Interestingly, Iodixanol treatment caused more significant effects. These findings suggest that conformational changes in blood components can occur, which can potentially lead to the increased prevalence of cardiovascular dysfunctions and blood clotting.https://www.mdpi.com/2075-4418/13/15/2523human bloodplasmaserumcontrast mediaIomeprolIobitridol |
| spellingShingle | Elek Telek Zoltán Ujfalusi Miklós Nyitrai Péter Bogner András Lukács Tímea Németh Gabriella Hild Gábor Hild Deconvolution Analysis of the Non-Ionic Iomeprol, Iobitridol and Iodixanol Contrast Media-Treated Human Whole Blood Thermograms: A Comparative Study Diagnostics human blood plasma serum contrast media Iomeprol Iobitridol |
| title | Deconvolution Analysis of the Non-Ionic Iomeprol, Iobitridol and Iodixanol Contrast Media-Treated Human Whole Blood Thermograms: A Comparative Study |
| title_full | Deconvolution Analysis of the Non-Ionic Iomeprol, Iobitridol and Iodixanol Contrast Media-Treated Human Whole Blood Thermograms: A Comparative Study |
| title_fullStr | Deconvolution Analysis of the Non-Ionic Iomeprol, Iobitridol and Iodixanol Contrast Media-Treated Human Whole Blood Thermograms: A Comparative Study |
| title_full_unstemmed | Deconvolution Analysis of the Non-Ionic Iomeprol, Iobitridol and Iodixanol Contrast Media-Treated Human Whole Blood Thermograms: A Comparative Study |
| title_short | Deconvolution Analysis of the Non-Ionic Iomeprol, Iobitridol and Iodixanol Contrast Media-Treated Human Whole Blood Thermograms: A Comparative Study |
| title_sort | deconvolution analysis of the non ionic iomeprol iobitridol and iodixanol contrast media treated human whole blood thermograms a comparative study |
| topic | human blood plasma serum contrast media Iomeprol Iobitridol |
| url | https://www.mdpi.com/2075-4418/13/15/2523 |
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