P.27 Mechanisms of NADPH Oxidase Participation in the Regulation of Diaphragm Artery Contractile Responses
Reactive oxygen species (ROS) produced by NADPH-oxidase (NOX) participate in vascular tone control, but their effects in the arteries of respiratory muscles is poorly understood. Possible targets of vasoregulatory ROS influence are NO-pathway in the endothelium and Rho-kinase pathway in smooth muscl...
| Main Authors: | , , , |
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| Format: | Article |
| Language: | English |
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BMC
2020-12-01
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| Series: | Artery Research |
| Subjects: | |
| Online Access: | https://www.atlantis-press.com/article/125950070/view |
| _version_ | 1828499179509383168 |
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| author | Anna Borzykh Ilya Kuzmin Olga Vinogradova Olga Tarasova |
| author_facet | Anna Borzykh Ilya Kuzmin Olga Vinogradova Olga Tarasova |
| author_sort | Anna Borzykh |
| collection | DOAJ |
| description | Reactive oxygen species (ROS) produced by NADPH-oxidase (NOX) participate in vascular tone control, but their effects in the arteries of respiratory muscles is poorly understood. Possible targets of vasoregulatory ROS influence are NO-pathway in the endothelium and Rho-kinase pathway in smooth muscle cells. Therefore, the aim of this study was to evaluate the interaction of NOX-dependent control with NO- and Rho-kinase signaling pathways in rat diaphragm arteries (DA).
Methods: The segments of DA were isolated from male Wistar rats and mounted in wire myograph (DMT A/S). We studied the effects of NOX inhibitor VAS2870 (1 μM) on contractile responses to α1-adrenergic agonist methoxamine in the absence and in the presence of NO synthase (L-NNA 100 μM) or Rho-kinase (Y27632, 3 μM) inhibitors as well as in the presence of NO donor DEA/NO.
Results: VAS2879 prominently attenuated the contractile responses of DA to methoxamine (30% decrease of the area under the concentration-response curve). L-NNA and Y27632 increased and decreased methoxamine-induced contraction of DA, respectively. L-NNA did not change the effects of VAS2870 and the sensitivity to DEA/NO did not differ in arteries with active and inhibited NOX. Along with that Y27632 eliminated the effects of VAS2879 on DA contractile responses to methoxamine.
Conclusions: We showed that NOX-produced ROS potentiate contractile responses of DA. ROS did not affect the activity of NO-pathway in either endothelial or smooth muscle cells of DA. However, ROS modulate the activity of the Rho-kinase pathway in DA smooth muscle cells. Supported by RSF (project No 19-75-00060). |
| first_indexed | 2024-12-11T13:19:17Z |
| format | Article |
| id | doaj.art-a299673c9e2440e780f153ceab4090f5 |
| institution | Directory Open Access Journal |
| issn | 1876-4401 |
| language | English |
| last_indexed | 2024-12-11T13:19:17Z |
| publishDate | 2020-12-01 |
| publisher | BMC |
| record_format | Article |
| series | Artery Research |
| spelling | doaj.art-a299673c9e2440e780f153ceab4090f52022-12-22T01:05:54ZengBMCArtery Research1876-44012020-12-0126Supplement 110.2991/artres.k.201209.040P.27 Mechanisms of NADPH Oxidase Participation in the Regulation of Diaphragm Artery Contractile ResponsesAnna BorzykhIlya KuzminOlga VinogradovaOlga TarasovaReactive oxygen species (ROS) produced by NADPH-oxidase (NOX) participate in vascular tone control, but their effects in the arteries of respiratory muscles is poorly understood. Possible targets of vasoregulatory ROS influence are NO-pathway in the endothelium and Rho-kinase pathway in smooth muscle cells. Therefore, the aim of this study was to evaluate the interaction of NOX-dependent control with NO- and Rho-kinase signaling pathways in rat diaphragm arteries (DA). Methods: The segments of DA were isolated from male Wistar rats and mounted in wire myograph (DMT A/S). We studied the effects of NOX inhibitor VAS2870 (1 μM) on contractile responses to α1-adrenergic agonist methoxamine in the absence and in the presence of NO synthase (L-NNA 100 μM) or Rho-kinase (Y27632, 3 μM) inhibitors as well as in the presence of NO donor DEA/NO. Results: VAS2879 prominently attenuated the contractile responses of DA to methoxamine (30% decrease of the area under the concentration-response curve). L-NNA and Y27632 increased and decreased methoxamine-induced contraction of DA, respectively. L-NNA did not change the effects of VAS2870 and the sensitivity to DEA/NO did not differ in arteries with active and inhibited NOX. Along with that Y27632 eliminated the effects of VAS2879 on DA contractile responses to methoxamine. Conclusions: We showed that NOX-produced ROS potentiate contractile responses of DA. ROS did not affect the activity of NO-pathway in either endothelial or smooth muscle cells of DA. However, ROS modulate the activity of the Rho-kinase pathway in DA smooth muscle cells. Supported by RSF (project No 19-75-00060).https://www.atlantis-press.com/article/125950070/viewArterydiaphragmNADPH oxidase |
| spellingShingle | Anna Borzykh Ilya Kuzmin Olga Vinogradova Olga Tarasova P.27 Mechanisms of NADPH Oxidase Participation in the Regulation of Diaphragm Artery Contractile Responses Artery Research Artery diaphragm NADPH oxidase |
| title | P.27 Mechanisms of NADPH Oxidase Participation in the Regulation of Diaphragm Artery Contractile Responses |
| title_full | P.27 Mechanisms of NADPH Oxidase Participation in the Regulation of Diaphragm Artery Contractile Responses |
| title_fullStr | P.27 Mechanisms of NADPH Oxidase Participation in the Regulation of Diaphragm Artery Contractile Responses |
| title_full_unstemmed | P.27 Mechanisms of NADPH Oxidase Participation in the Regulation of Diaphragm Artery Contractile Responses |
| title_short | P.27 Mechanisms of NADPH Oxidase Participation in the Regulation of Diaphragm Artery Contractile Responses |
| title_sort | p 27 mechanisms of nadph oxidase participation in the regulation of diaphragm artery contractile responses |
| topic | Artery diaphragm NADPH oxidase |
| url | https://www.atlantis-press.com/article/125950070/view |
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