Hibberdia magna (Chrysophyceae): a promising freshwater fucoxanthin and polyunsaturated fatty acid producer
Abstract Background Algae are prominent producers of carotenoids and polyunsaturated fatty acids which are greatly prized in the food and pharmaceutic industry. Fucoxanthin represents a notable high-value carotenoid produced exclusively by algae. Its benefits range far beyond just antioxidant activi...
| Main Authors: | , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2023-04-01
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| Series: | Microbial Cell Factories |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s12934-023-02061-x |
| _version_ | 1797840736749617152 |
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| author | Antonín Střížek Pavel Přibyl Martin Lukeš Tomáš Grivalský Jiří Kopecký Tomáš Galica Pavel Hrouzek |
| author_facet | Antonín Střížek Pavel Přibyl Martin Lukeš Tomáš Grivalský Jiří Kopecký Tomáš Galica Pavel Hrouzek |
| author_sort | Antonín Střížek |
| collection | DOAJ |
| description | Abstract Background Algae are prominent producers of carotenoids and polyunsaturated fatty acids which are greatly prized in the food and pharmaceutic industry. Fucoxanthin represents a notable high-value carotenoid produced exclusively by algae. Its benefits range far beyond just antioxidant activity and include cancer prevention, anti-diabetes, anti-obesity, and many other positive effects. Accordingly, large-scale microalgae cultivation to produce fucoxanthin and polyunsaturated fatty acids is still under intensive development in the commercial and academic sectors. Industrially exploitable strains are predominantly derived from marine species while comparable freshwater fucoxanthin producers have yet to be explored. Results In this study, we searched for freshwater fucoxanthin producers among photoautotrophic flagellates including members of the class Chrysophyceae. The initial screening turned our attention to the chrysophyte alga Hibberdia magna. We performed a comprehensive cultivation experiments using a temperature × light cross-gradient to assess the impact of these conditions on the target compounds productivity. Here we present the observations that H. magna simultaneously produces fucoxanthin (max. 1.2% dry biomass) and polyunsaturated fatty acids (max. ~ 9.9% dry biomass) and is accessible to routine cultivation in lab-scale conditions. The highest biomass yields were 3.73 g L−1 accompanied by maximal volumetric productivity of 0.54 g L−1 d−1 which are comparable values to marine microalgae fucoxanthin producers in phototrophic mode. H. magna demonstrated different optimal conditions for biomass, fucoxanthin, and fatty acid accumulation. While maximal fucoxanthin productivities were obtained in dim light and moderate temperatures (23 °C× 80 µmol m−2 s−1), the highest PUFA and overall biomass productivities were found in low temperature and high light (17–20 °C × 320–480 µmol m−2 s−1). Thus, a smart biotechnology setup should be designed to fully utilize H. magna biotechnological potential. Conclusions Our research brings pioneer insight into the biotechnology potential of freshwater autotrophic flagellates and highlights their ability to produce high-value compounds. Freshwater fucoxanthin-producing species are of special importance as the use of sea-water-based media may increase cultivation costs and prohibits inland microalgae production. |
| first_indexed | 2024-04-09T16:19:33Z |
| format | Article |
| id | doaj.art-a29ac43e768b4d33b4fb7380e6adcd55 |
| institution | Directory Open Access Journal |
| issn | 1475-2859 |
| language | English |
| last_indexed | 2024-04-09T16:19:33Z |
| publishDate | 2023-04-01 |
| publisher | BMC |
| record_format | Article |
| series | Microbial Cell Factories |
| spelling | doaj.art-a29ac43e768b4d33b4fb7380e6adcd552023-04-23T11:32:16ZengBMCMicrobial Cell Factories1475-28592023-04-0122111910.1186/s12934-023-02061-xHibberdia magna (Chrysophyceae): a promising freshwater fucoxanthin and polyunsaturated fatty acid producerAntonín Střížek0Pavel Přibyl1Martin Lukeš2Tomáš Grivalský3Jiří Kopecký4Tomáš Galica5Pavel Hrouzek6Laboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center AlgatechCentre for Phycology, Institute of Botany of the Czech Academy of SciencesLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center AlgatechLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center AlgatechLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center AlgatechLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center AlgatechLaboratory of Algal Biotechnology, Institute of Microbiology of the Czech Academy of Sciences - Center AlgatechAbstract Background Algae are prominent producers of carotenoids and polyunsaturated fatty acids which are greatly prized in the food and pharmaceutic industry. Fucoxanthin represents a notable high-value carotenoid produced exclusively by algae. Its benefits range far beyond just antioxidant activity and include cancer prevention, anti-diabetes, anti-obesity, and many other positive effects. Accordingly, large-scale microalgae cultivation to produce fucoxanthin and polyunsaturated fatty acids is still under intensive development in the commercial and academic sectors. Industrially exploitable strains are predominantly derived from marine species while comparable freshwater fucoxanthin producers have yet to be explored. Results In this study, we searched for freshwater fucoxanthin producers among photoautotrophic flagellates including members of the class Chrysophyceae. The initial screening turned our attention to the chrysophyte alga Hibberdia magna. We performed a comprehensive cultivation experiments using a temperature × light cross-gradient to assess the impact of these conditions on the target compounds productivity. Here we present the observations that H. magna simultaneously produces fucoxanthin (max. 1.2% dry biomass) and polyunsaturated fatty acids (max. ~ 9.9% dry biomass) and is accessible to routine cultivation in lab-scale conditions. The highest biomass yields were 3.73 g L−1 accompanied by maximal volumetric productivity of 0.54 g L−1 d−1 which are comparable values to marine microalgae fucoxanthin producers in phototrophic mode. H. magna demonstrated different optimal conditions for biomass, fucoxanthin, and fatty acid accumulation. While maximal fucoxanthin productivities were obtained in dim light and moderate temperatures (23 °C× 80 µmol m−2 s−1), the highest PUFA and overall biomass productivities were found in low temperature and high light (17–20 °C × 320–480 µmol m−2 s−1). Thus, a smart biotechnology setup should be designed to fully utilize H. magna biotechnological potential. Conclusions Our research brings pioneer insight into the biotechnology potential of freshwater autotrophic flagellates and highlights their ability to produce high-value compounds. Freshwater fucoxanthin-producing species are of special importance as the use of sea-water-based media may increase cultivation costs and prohibits inland microalgae production.https://doi.org/10.1186/s12934-023-02061-xMicroalgaeFucoxanthinChrysophyceaeHibberdia magnaPolyunsaturated fatty acidsVolumetric productivity |
| spellingShingle | Antonín Střížek Pavel Přibyl Martin Lukeš Tomáš Grivalský Jiří Kopecký Tomáš Galica Pavel Hrouzek Hibberdia magna (Chrysophyceae): a promising freshwater fucoxanthin and polyunsaturated fatty acid producer Microbial Cell Factories Microalgae Fucoxanthin Chrysophyceae Hibberdia magna Polyunsaturated fatty acids Volumetric productivity |
| title | Hibberdia magna (Chrysophyceae): a promising freshwater fucoxanthin and polyunsaturated fatty acid producer |
| title_full | Hibberdia magna (Chrysophyceae): a promising freshwater fucoxanthin and polyunsaturated fatty acid producer |
| title_fullStr | Hibberdia magna (Chrysophyceae): a promising freshwater fucoxanthin and polyunsaturated fatty acid producer |
| title_full_unstemmed | Hibberdia magna (Chrysophyceae): a promising freshwater fucoxanthin and polyunsaturated fatty acid producer |
| title_short | Hibberdia magna (Chrysophyceae): a promising freshwater fucoxanthin and polyunsaturated fatty acid producer |
| title_sort | hibberdia magna chrysophyceae a promising freshwater fucoxanthin and polyunsaturated fatty acid producer |
| topic | Microalgae Fucoxanthin Chrysophyceae Hibberdia magna Polyunsaturated fatty acids Volumetric productivity |
| url | https://doi.org/10.1186/s12934-023-02061-x |
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