Molecular Diagnosis and Pathology of Chicken Infectious Anemia in Commercial White Leghorn Layer Flocks in Pakistan
The present field study was conducted for confirmation of chicken infectious anemia (CIA) in White Leghorn (WL) commercial layer birds. A total of 60 farms were investigated. Birds from each farm ware necropsied for the presence of lesions on different visceral organs. Samples of blood and different...
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| Format: | Article |
| Language: | English |
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University of Agriculture, Faisalabad
2013-07-01
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| Series: | Pakistan Veterinary Journal |
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| Online Access: | http://pvj.com.pk/pdf-files/33_3/378-381.pdf |
| _version_ | 1818664558309408768 |
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| author | Najm-ul-Islam1, Muhammad Kashif Saleemi2*, Muhammad Zargham Khan2, Salman Latif Butt2, Ahrar Khan2, Ijaz Javed3, Faisal Saeed Awan4and ShahidRafique5 |
| author_facet | Najm-ul-Islam1, Muhammad Kashif Saleemi2*, Muhammad Zargham Khan2, Salman Latif Butt2, Ahrar Khan2, Ijaz Javed3, Faisal Saeed Awan4and ShahidRafique5 |
| author_sort | Najm-ul-Islam1, Muhammad Kashif Saleemi2*, Muhammad Zargham Khan2, Salman Latif Butt2, Ahrar Khan2, Ijaz Javed3, Faisal Saeed Awan4and ShahidRafique5 |
| collection | DOAJ |
| description | The present field study was conducted for confirmation of chicken infectious anemia (CIA) in White Leghorn (WL) commercial layer birds. A total of 60 farms were investigated. Birds from each farm ware necropsied for the presence of lesions on different visceral organs. Samples of blood and different tissues were collected for hematology, histopathology, DNA extraction and PCR amplification using specific primers for CIA virus. There was severe anemia indicated by low hematocrit values (10.9 to 17.2%) and hemoglobin concentration (5.3 to6.7 g/dl). The petechial hemorrhages were present on subcutaneous tissue, epicardium, endocardium and gizzard mucosa. The liver and bone marrow were pale in appearance. The mortality ranged from 5 to14 % on different farms. Samples of liver and spleen from 15 farms were subjected to PCR analysis for CIAV infection by amplifying the186-bp region on highly conserved VP-2 coding gene using CAV1 and CAV2 primer pair. Presence of CIAV was confirmed in 67 and 33 percent samples of liver and spleen, respectively. A total of 13/15 farms (87%) were found positive for CIA. The results of present study confirmed the presence of CAV infection in WL commercial layer birds in current outbreak. It is concluded that extensive molecular epidemiological studies are required at national level to assess the prevalence of disease. Breeder flocks should be vaccinated to control CIA in commercial layer flocks. |
| first_indexed | 2024-12-17T05:34:39Z |
| format | Article |
| id | doaj.art-a29aff8e69ec46a5a60e149fe897fa92 |
| institution | Directory Open Access Journal |
| issn | 0253-8318 |
| language | English |
| last_indexed | 2024-12-17T05:34:39Z |
| publishDate | 2013-07-01 |
| publisher | University of Agriculture, Faisalabad |
| record_format | Article |
| series | Pakistan Veterinary Journal |
| spelling | doaj.art-a29aff8e69ec46a5a60e149fe897fa922022-12-21T22:01:39ZengUniversity of Agriculture, FaisalabadPakistan Veterinary Journal0253-83182013-07-01333378381Molecular Diagnosis and Pathology of Chicken Infectious Anemia in Commercial White Leghorn Layer Flocks in PakistanNajm-ul-Islam1, Muhammad Kashif Saleemi2*, Muhammad Zargham Khan2, Salman Latif Butt2, Ahrar Khan2, Ijaz Javed3, Faisal Saeed Awan4and ShahidRafique5The present field study was conducted for confirmation of chicken infectious anemia (CIA) in White Leghorn (WL) commercial layer birds. A total of 60 farms were investigated. Birds from each farm ware necropsied for the presence of lesions on different visceral organs. Samples of blood and different tissues were collected for hematology, histopathology, DNA extraction and PCR amplification using specific primers for CIA virus. There was severe anemia indicated by low hematocrit values (10.9 to 17.2%) and hemoglobin concentration (5.3 to6.7 g/dl). The petechial hemorrhages were present on subcutaneous tissue, epicardium, endocardium and gizzard mucosa. The liver and bone marrow were pale in appearance. The mortality ranged from 5 to14 % on different farms. Samples of liver and spleen from 15 farms were subjected to PCR analysis for CIAV infection by amplifying the186-bp region on highly conserved VP-2 coding gene using CAV1 and CAV2 primer pair. Presence of CIAV was confirmed in 67 and 33 percent samples of liver and spleen, respectively. A total of 13/15 farms (87%) were found positive for CIA. The results of present study confirmed the presence of CAV infection in WL commercial layer birds in current outbreak. It is concluded that extensive molecular epidemiological studies are required at national level to assess the prevalence of disease. Breeder flocks should be vaccinated to control CIA in commercial layer flocks.http://pvj.com.pk/pdf-files/33_3/378-381.pdfBone marrowLiverPolymerase chain reactionSpleenWhite Leghorn |
| spellingShingle | Najm-ul-Islam1, Muhammad Kashif Saleemi2*, Muhammad Zargham Khan2, Salman Latif Butt2, Ahrar Khan2, Ijaz Javed3, Faisal Saeed Awan4and ShahidRafique5 Molecular Diagnosis and Pathology of Chicken Infectious Anemia in Commercial White Leghorn Layer Flocks in Pakistan Pakistan Veterinary Journal Bone marrow Liver Polymerase chain reaction Spleen White Leghorn |
| title | Molecular Diagnosis and Pathology of Chicken Infectious Anemia in Commercial White Leghorn Layer Flocks in Pakistan |
| title_full | Molecular Diagnosis and Pathology of Chicken Infectious Anemia in Commercial White Leghorn Layer Flocks in Pakistan |
| title_fullStr | Molecular Diagnosis and Pathology of Chicken Infectious Anemia in Commercial White Leghorn Layer Flocks in Pakistan |
| title_full_unstemmed | Molecular Diagnosis and Pathology of Chicken Infectious Anemia in Commercial White Leghorn Layer Flocks in Pakistan |
| title_short | Molecular Diagnosis and Pathology of Chicken Infectious Anemia in Commercial White Leghorn Layer Flocks in Pakistan |
| title_sort | molecular diagnosis and pathology of chicken infectious anemia in commercial white leghorn layer flocks in pakistan |
| topic | Bone marrow Liver Polymerase chain reaction Spleen White Leghorn |
| url | http://pvj.com.pk/pdf-files/33_3/378-381.pdf |
| work_keys_str_mv | AT najmulislam1muhammadkashifsaleemi2muhammadzarghamkhan2salmanlatifbutt2ahrarkhan2ijazjaved3faisalsaeedawan4andshahidrafique5 moleculardiagnosisandpathologyofchickeninfectiousanemiaincommercialwhiteleghornlayerflocksinpakistan |