Detection of Second Line Drug Resistance among Drug Resistant Mycobacterium Tuberculosis Isolates in Botswana
The emergence and transmission of multidrug resistant (MDR) and extensively drug resistant (XDR) <i>Mycobacterium tuberculosis (M.tb)</i> strains is a threat to global tuberculosis (TB) control. The early detection of drug resistance is critical for patient management. The aim of this st...
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| Format: | Article |
| Language: | English |
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MDPI AG
2019-10-01
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| Series: | Pathogens |
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| Online Access: | https://www.mdpi.com/2076-0817/8/4/208 |
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| author | Tuelo Mogashoa Pinkie Melamu Brigitta Derendinger Serej D. Ley Elizabeth M. Streicher Thato Iketleng Lucy Mupfumi Margaret Mokomane Botshelo Kgwaadira Goabaone Rankgoane-Pono Thusoyaone T. Tsholofelo Ishmael Kasvosve Sikhulile Moyo Robin M. Warren Simani Gaseitsiwe |
| author_facet | Tuelo Mogashoa Pinkie Melamu Brigitta Derendinger Serej D. Ley Elizabeth M. Streicher Thato Iketleng Lucy Mupfumi Margaret Mokomane Botshelo Kgwaadira Goabaone Rankgoane-Pono Thusoyaone T. Tsholofelo Ishmael Kasvosve Sikhulile Moyo Robin M. Warren Simani Gaseitsiwe |
| author_sort | Tuelo Mogashoa |
| collection | DOAJ |
| description | The emergence and transmission of multidrug resistant (MDR) and extensively drug resistant (XDR) <i>Mycobacterium tuberculosis (M.tb)</i> strains is a threat to global tuberculosis (TB) control. The early detection of drug resistance is critical for patient management. The aim of this study was to determine the proportion of isolates with additional second-line resistance among rifampicin and isoniazid resistant and MDR-TB isolates. A total of 66 <i>M.tb</i> isolates received at the National Tuberculosis Reference Laboratory between March 2012 and October 2013 with resistance to isoniazid, rifampicin or both were analyzed in this study. The genotypes of the <i>M.tb</i> isolates were determined by spoligotyping and second-line drug susceptibility testing was done using the Hain Genotype MTBDR<i>sl</i> line probe assay version 2.0. The treatment outcomes were defined according to the Botswana national and World Health Organization (WHO) guidelines. Of the 57 isolates analyzed, 33 (58%) were MDR-TB, 4 (7%) were additionally resistant to flouroquinolones and 3 (5%) were resistant to both fluoroquinolones and second-line injectable drugs. The most common fluoroquinolone resistance-conferring mutation detected was <i>gyrA</i> A90V. All XDR-TB cases remained smear or culture positive throughout the treatment. Our study findings indicate the importance of monitoring drug resistant TB cases to ensure rapid detection of second-line drug resistance. |
| first_indexed | 2024-04-11T22:21:12Z |
| format | Article |
| id | doaj.art-a29c3f93a2eb4c73b5238340ac2b2491 |
| institution | Directory Open Access Journal |
| issn | 2076-0817 |
| language | English |
| last_indexed | 2024-04-11T22:21:12Z |
| publishDate | 2019-10-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | Pathogens |
| spelling | doaj.art-a29c3f93a2eb4c73b5238340ac2b24912022-12-22T04:00:06ZengMDPI AGPathogens2076-08172019-10-018420810.3390/pathogens8040208pathogens8040208Detection of Second Line Drug Resistance among Drug Resistant Mycobacterium Tuberculosis Isolates in BotswanaTuelo Mogashoa0Pinkie Melamu1Brigitta Derendinger2Serej D. Ley3Elizabeth M. Streicher4Thato Iketleng5Lucy Mupfumi6Margaret Mokomane7Botshelo Kgwaadira8Goabaone Rankgoane-Pono9Thusoyaone T. Tsholofelo10Ishmael Kasvosve11Sikhulile Moyo12Robin M. Warren13Simani Gaseitsiwe14Department of Medical Laboratory Sciences, Faculty of Health Sciences, University of Botswana, Gaborone 0022, BotswanaBotswana Harvard AIDS Institute Partnership, Gaborone 0000, BotswanaDST-NRF Centre of Excellence in Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, Cape Town 7505, South AfricaDST-NRF Centre of Excellence in Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, Cape Town 7505, South AfricaDST-NRF Centre of Excellence in Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, Cape Town 7505, South AfricaBotswana Harvard AIDS Institute Partnership, Gaborone 0000, BotswanaDepartment of Medical Laboratory Sciences, Faculty of Health Sciences, University of Botswana, Gaborone 0022, BotswanaNational Tuberculosis Reference Laboratory, Ministry of Health and Wellness, Gaborone 0000, BotswanaBotswana National Tuberculosis Programme, Ministry of Health and Wellness, Gaborone 0000, BotswanaBotswana National Tuberculosis Programme, Ministry of Health and Wellness, Gaborone 0000, BotswanaBotswana National Tuberculosis Programme, Ministry of Health and Wellness, Gaborone 0000, BotswanaDepartment of Medical Laboratory Sciences, Faculty of Health Sciences, University of Botswana, Gaborone 0022, BotswanaBotswana Harvard AIDS Institute Partnership, Gaborone 0000, BotswanaDST-NRF Centre of Excellence in Biomedical Tuberculosis Research, South African Medical Research Council Centre for Tuberculosis Research, Division of Molecular Biology and Human Genetics, Faculty of Medicine and Health Sciences, Stellenbosch University, Tygerberg, Cape Town 7505, South AfricaBotswana Harvard AIDS Institute Partnership, Gaborone 0000, BotswanaThe emergence and transmission of multidrug resistant (MDR) and extensively drug resistant (XDR) <i>Mycobacterium tuberculosis (M.tb)</i> strains is a threat to global tuberculosis (TB) control. The early detection of drug resistance is critical for patient management. The aim of this study was to determine the proportion of isolates with additional second-line resistance among rifampicin and isoniazid resistant and MDR-TB isolates. A total of 66 <i>M.tb</i> isolates received at the National Tuberculosis Reference Laboratory between March 2012 and October 2013 with resistance to isoniazid, rifampicin or both were analyzed in this study. The genotypes of the <i>M.tb</i> isolates were determined by spoligotyping and second-line drug susceptibility testing was done using the Hain Genotype MTBDR<i>sl</i> line probe assay version 2.0. The treatment outcomes were defined according to the Botswana national and World Health Organization (WHO) guidelines. Of the 57 isolates analyzed, 33 (58%) were MDR-TB, 4 (7%) were additionally resistant to flouroquinolones and 3 (5%) were resistant to both fluoroquinolones and second-line injectable drugs. The most common fluoroquinolone resistance-conferring mutation detected was <i>gyrA</i> A90V. All XDR-TB cases remained smear or culture positive throughout the treatment. Our study findings indicate the importance of monitoring drug resistant TB cases to ensure rapid detection of second-line drug resistance.https://www.mdpi.com/2076-0817/8/4/208<i>mycobacterium tuberculosis</i>line probe assaysecond-line drugsdrug resistancexdr-tbmdr-tb |
| spellingShingle | Tuelo Mogashoa Pinkie Melamu Brigitta Derendinger Serej D. Ley Elizabeth M. Streicher Thato Iketleng Lucy Mupfumi Margaret Mokomane Botshelo Kgwaadira Goabaone Rankgoane-Pono Thusoyaone T. Tsholofelo Ishmael Kasvosve Sikhulile Moyo Robin M. Warren Simani Gaseitsiwe Detection of Second Line Drug Resistance among Drug Resistant Mycobacterium Tuberculosis Isolates in Botswana Pathogens <i>mycobacterium tuberculosis</i> line probe assay second-line drugs drug resistance xdr-tb mdr-tb |
| title | Detection of Second Line Drug Resistance among Drug Resistant Mycobacterium Tuberculosis Isolates in Botswana |
| title_full | Detection of Second Line Drug Resistance among Drug Resistant Mycobacterium Tuberculosis Isolates in Botswana |
| title_fullStr | Detection of Second Line Drug Resistance among Drug Resistant Mycobacterium Tuberculosis Isolates in Botswana |
| title_full_unstemmed | Detection of Second Line Drug Resistance among Drug Resistant Mycobacterium Tuberculosis Isolates in Botswana |
| title_short | Detection of Second Line Drug Resistance among Drug Resistant Mycobacterium Tuberculosis Isolates in Botswana |
| title_sort | detection of second line drug resistance among drug resistant mycobacterium tuberculosis isolates in botswana |
| topic | <i>mycobacterium tuberculosis</i> line probe assay second-line drugs drug resistance xdr-tb mdr-tb |
| url | https://www.mdpi.com/2076-0817/8/4/208 |
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