An Attempt to Make Platelet Rich Fibrin from Bone Marrow Aspirate
Introduction: Platelet Rich Fibrin (PRF) was developed as a new generation of platelet concentration from peripheral blood. Method to make PRF is simple. It is easy to handle with its moderate firmness. Histologically, platelets and nucleated cells are packed along the yellow-red border. Bone ma...
| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
JCDR Research and Publications Private Limited
2017-08-01
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| Series: | Journal of Clinical and Diagnostic Research |
| Subjects: | |
| Online Access: | https://jcdr.net/articles/PDF/10380/27006_CE[Ra1]_F(SS)_PF1_(DS_VT_PY)_PFA(DS_VT_GG).pdf |
| Summary: | Introduction: Platelet Rich Fibrin (PRF) was developed as
a new generation of platelet concentration from peripheral
blood. Method to make PRF is simple. It is easy to handle with
its moderate firmness. Histologically, platelets and nucleated
cells are packed along the yellow-red border. Bone marrow
aspirate contains bone marrow cells that potentially work for
tissue regeneration, and platelets which contain growth factors.
The specific gravities of them are comparable. It implies that,
if it is possible to make PRF from bone marrow aspirate, then
high concentration of platelets and bone marrow cells can be
obtained simultaneously by taking out yellow-red interface of it.
Aim: To find out a method to make PRF from bone marrow
aspirate.
Materials and Methods: Iliac crest of rabbits were punctured
and aspirated with or without anti-coagulant, under general
anaesthesia. The bone marrow aspirate was centrifuged in glass
tubes. For the bone marrow aspirate taken with anti-coagulant,
calcium chloride was added just before centrifugation. Products
were taken out and observed grossly. The products were fixed
with formaldehyde and observed histologically.
Results: Coagulated gels with two-toned colour were obtained
by all methods. In the gels without anti-coagulant, interfaces
between two colours were obscure. Histologically, platelets
and nucleated cells scattered as clusters. Filtering caused
haemolysis and reduced the yield of the product. With the
aspirate taken with anti-coagulant, platelets and nucleated cells
formed a band along the interface.
Conclusion: PRF can be made from bone marrow aspirate by
adding anti-coagulant in aspiration and reversed with calcium
chloride just before centrifugation. |
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| ISSN: | 2249-782X 0973-709X |