Circulating MicroRNAs in Plasma Decrease in Response to Sarcopenia in the Elderly
sarcopenia has been defined as the aging-related disease with the declined mass, strength, and function of skeletal muscle, which is a major cause of morbidity and mortality in elders. Current diagnostic criteria of sarcopenia have not been agreed internationally, and the clinical diagnostic biomark...
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| Format: | Article |
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Frontiers Media S.A.
2020-03-01
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| Series: | Frontiers in Genetics |
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| Online Access: | https://www.frontiersin.org/article/10.3389/fgene.2020.00167/full |
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| author | Nana He Nana He Yue Lin Zhang Yue Zhang Beili Feng Zaixing Zheng Dongjuan Wang Shun Zhang Shun Zhang Qi Guo Honghua Ye |
| author_facet | Nana He Nana He Yue Lin Zhang Yue Zhang Beili Feng Zaixing Zheng Dongjuan Wang Shun Zhang Shun Zhang Qi Guo Honghua Ye |
| author_sort | Nana He |
| collection | DOAJ |
| description | sarcopenia has been defined as the aging-related disease with the declined mass, strength, and function of skeletal muscle, which is a major cause of morbidity and mortality in elders. Current diagnostic criteria of sarcopenia have not been agreed internationally, and the clinical diagnostic biomarkers for sarcopenia have not been identified. Circulating miRNAs (miRNAs, miRs) have recently been characterized as novel biomarkers for sarcopenia. However, the change of circulating miRNAs in response to sarcopenia are still not fully understood. Here, we enrolled a total of 93 elderly patients clinically diagnosed with sarcopenia and matching 93 non-sarcopenia elderly in this study. Specifically, levels of candidate circulating miRNAs which were involved in angiogenesis, inflammation and enriched in muscle and/or cardiac tissues were detected in these two groups. In small-sample screening experiments, plasma miR-155, miR-208b, miR-222, miR-210, miR-328, and miR-499 levels were significantly down-regulated in sarcopenia compared to those who non-sarcopenia. In contrast, miR-1, mir-133a, miR-133b, miR-21, miR-146a, miR-126, miR-221, and miR-20a were not changed significantly. Subsequently, we expanded the sample size to further detection and verification, and found that plasma miR-155, miR-208b, miR-222, miR-210, miR-328, and miR-499 levels in the sarcopenia group were significantly reduced compared to the non-sarcoma group, which is consistent with the results of the small-sample screening experiment. In addition, we showed that ASM/Height2, handgrip strength, knee extension and 4-meter velocity in sarcopenia group were significantly lower than those in non-sarcopenia group. Here we correlated the decrease of miR-208b, miR-499, miR-155, miR-222, miR-328, and miR-210 in sarcopenia group and non-sarcopenia group with diagnostic indexes of sarcopenia (ASM/Height2, Handgrip strength and 4-meter velocity) after adjusting sex. The results showed that miR-208b and miR-155 changes were significantly correlated with handgrip strength in woman, miR-208b, miR-499, and miR-222 changes were significantly correlated with ASM/Height2 in man, while other miRNAs changes did not show a strong correlation with these diagnostic indexes. In conclusion, plasma miR-208b, miR-499, miR-155, miR-222, miR-328, and miR-210 decrease in response to sarcopenia in the elderly. Although further studies are needed to clarify the potential use of circulating miRNAs as biomarkers of sarcopenia, present findings set the stage for defining circulating miRNAs as biomarkers and suggesting their physiological roles in elderly with sarcopenia. |
| first_indexed | 2024-12-14T20:13:25Z |
| format | Article |
| id | doaj.art-a29ec2dd58094e0fbb2ab164d09985cc |
| institution | Directory Open Access Journal |
| issn | 1664-8021 |
| language | English |
| last_indexed | 2024-12-14T20:13:25Z |
| publishDate | 2020-03-01 |
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| series | Frontiers in Genetics |
| spelling | doaj.art-a29ec2dd58094e0fbb2ab164d09985cc2022-12-21T22:48:54ZengFrontiers Media S.A.Frontiers in Genetics1664-80212020-03-011110.3389/fgene.2020.00167519243Circulating MicroRNAs in Plasma Decrease in Response to Sarcopenia in the ElderlyNana He0Nana He1Yue Lin Zhang2Yue Zhang3Beili Feng4Zaixing Zheng5Dongjuan Wang6Shun Zhang7Shun Zhang8Qi Guo9Honghua Ye10Department of Experimental Medical Science, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, ChinaKey Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province, Ningbo, ChinaDepartment of Cardiology, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, ChinaDepartment of Cardiology, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, ChinaDepartment of Cardiology, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, ChinaDepartment of Cardiology, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, ChinaDepartment of Cardiology, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, ChinaDepartment of Experimental Medical Science, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, ChinaKey Laboratory of Diagnosis and Treatment of Digestive System Tumors of Zhejiang Province, Ningbo, ChinaShanghai University of Medicine and Health Sciences Affiliated Zhoupu Hospital, Shanghai, ChinaDepartment of Cardiology, HwaMei Hospital, University of Chinese Academy of Sciences, Ningbo, Chinasarcopenia has been defined as the aging-related disease with the declined mass, strength, and function of skeletal muscle, which is a major cause of morbidity and mortality in elders. Current diagnostic criteria of sarcopenia have not been agreed internationally, and the clinical diagnostic biomarkers for sarcopenia have not been identified. Circulating miRNAs (miRNAs, miRs) have recently been characterized as novel biomarkers for sarcopenia. However, the change of circulating miRNAs in response to sarcopenia are still not fully understood. Here, we enrolled a total of 93 elderly patients clinically diagnosed with sarcopenia and matching 93 non-sarcopenia elderly in this study. Specifically, levels of candidate circulating miRNAs which were involved in angiogenesis, inflammation and enriched in muscle and/or cardiac tissues were detected in these two groups. In small-sample screening experiments, plasma miR-155, miR-208b, miR-222, miR-210, miR-328, and miR-499 levels were significantly down-regulated in sarcopenia compared to those who non-sarcopenia. In contrast, miR-1, mir-133a, miR-133b, miR-21, miR-146a, miR-126, miR-221, and miR-20a were not changed significantly. Subsequently, we expanded the sample size to further detection and verification, and found that plasma miR-155, miR-208b, miR-222, miR-210, miR-328, and miR-499 levels in the sarcopenia group were significantly reduced compared to the non-sarcoma group, which is consistent with the results of the small-sample screening experiment. In addition, we showed that ASM/Height2, handgrip strength, knee extension and 4-meter velocity in sarcopenia group were significantly lower than those in non-sarcopenia group. Here we correlated the decrease of miR-208b, miR-499, miR-155, miR-222, miR-328, and miR-210 in sarcopenia group and non-sarcopenia group with diagnostic indexes of sarcopenia (ASM/Height2, Handgrip strength and 4-meter velocity) after adjusting sex. The results showed that miR-208b and miR-155 changes were significantly correlated with handgrip strength in woman, miR-208b, miR-499, and miR-222 changes were significantly correlated with ASM/Height2 in man, while other miRNAs changes did not show a strong correlation with these diagnostic indexes. In conclusion, plasma miR-208b, miR-499, miR-155, miR-222, miR-328, and miR-210 decrease in response to sarcopenia in the elderly. Although further studies are needed to clarify the potential use of circulating miRNAs as biomarkers of sarcopenia, present findings set the stage for defining circulating miRNAs as biomarkers and suggesting their physiological roles in elderly with sarcopenia.https://www.frontiersin.org/article/10.3389/fgene.2020.00167/fullcirculating microRNAssarcopeniabiomarkerplasmaelderly |
| spellingShingle | Nana He Nana He Yue Lin Zhang Yue Zhang Beili Feng Zaixing Zheng Dongjuan Wang Shun Zhang Shun Zhang Qi Guo Honghua Ye Circulating MicroRNAs in Plasma Decrease in Response to Sarcopenia in the Elderly Frontiers in Genetics circulating microRNAs sarcopenia biomarker plasma elderly |
| title | Circulating MicroRNAs in Plasma Decrease in Response to Sarcopenia in the Elderly |
| title_full | Circulating MicroRNAs in Plasma Decrease in Response to Sarcopenia in the Elderly |
| title_fullStr | Circulating MicroRNAs in Plasma Decrease in Response to Sarcopenia in the Elderly |
| title_full_unstemmed | Circulating MicroRNAs in Plasma Decrease in Response to Sarcopenia in the Elderly |
| title_short | Circulating MicroRNAs in Plasma Decrease in Response to Sarcopenia in the Elderly |
| title_sort | circulating micrornas in plasma decrease in response to sarcopenia in the elderly |
| topic | circulating microRNAs sarcopenia biomarker plasma elderly |
| url | https://www.frontiersin.org/article/10.3389/fgene.2020.00167/full |
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