Mineral composition, principal polyphenolic components, and evaluation of the anti-inflammatory, analgesic, and antioxidant properties of Cytisus villosus Pourr leaf extracts
Cytisus villosus Pourr. (C. villosus) is a medicinal plant belonging to the Fabaceae family, which grows in the Mediterranean area. It is used in traditional medicine against diseases related to inflammation. The objective of the present study was to identify the mineral and polyphenolic composition...
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| Format: | Article |
| Language: | English |
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De Gruyter
2023-06-01
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| Series: | Open Chemistry |
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| Online Access: | https://doi.org/10.1515/chem-2022-0338 |
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| author | Zouhri Aziz El Menyiy Naoual El-mernissi Yahya Bouddine Toufik El-mernissi Rafik Amhamdi Hassan Elharrak Abdelhay Salamatullah Ahmad Mohammad Nafidi Hiba-Allah Khallouki Farid Bourhia Mohammed Hajji Lhoussain |
| author_facet | Zouhri Aziz El Menyiy Naoual El-mernissi Yahya Bouddine Toufik El-mernissi Rafik Amhamdi Hassan Elharrak Abdelhay Salamatullah Ahmad Mohammad Nafidi Hiba-Allah Khallouki Farid Bourhia Mohammed Hajji Lhoussain |
| author_sort | Zouhri Aziz |
| collection | DOAJ |
| description | Cytisus villosus Pourr. (C. villosus) is a medicinal plant belonging to the Fabaceae family, which grows in the Mediterranean area. It is used in traditional medicine against diseases related to inflammation. The objective of the present study was to identify the mineral and polyphenolic composition as well as to evaluate some biological properties including antioxidant, anti-inflammatory, and analgesic activities of C. villosus leaf aqueous extract. The chemical constituents were identified and quantified using ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) methods. The antioxidant properties of C. villosus leaves were tested using reducing power (RP), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) assays. The anti-inflammatory potency was evaluated in vitro and in vivo using the albumin denaturation test and the carrageenan test, respectively. Furthermore, the analgesic effect was performed in vivo using tail flick, acetic acid-induced contortion, and plantar tests. Mineralogical analysis revealed that potassium and calcium were the most abundant minerals. The analysis and quantification of the phytochemical composition using UPLC-ESI-MS/MS showed that quinic acid (57.478 ± 1.72 mg/kg) was the major compound of the aqueous extract, followed by salicylic acid (17.38 ± 0.2 mg/kg), isoquercetin (16.895 ± 1.01 mg/kg), and gallic acid (15.914 ± 1.51 mg/kg). The extracts showed potent antioxidant activity for all tests used. The highest antioxidant activity was recorded for the DPPH, ABTS and RP methods, with an IC50 of 3.94 ± 0.09, 2.88 ± 0.07, and 1.94 ± 0.10 μg/mL, respectively. Additionally, using the most frequent analgesic assays, the aqueous extract at a dose of 500 mg/kg exhibited a potent analgesic activity. Notably, an interesting inhibition of albumin denaturation was recorded with an IC50 of 383.94 μg/mL, corroborating the in vivo test. Overall, the results presented here may represent a scientific basis for the traditional use of C. villosus in the treatment of inflammation-related diseases. |
| first_indexed | 2024-03-13T04:42:33Z |
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| language | English |
| last_indexed | 2024-03-13T04:42:33Z |
| publishDate | 2023-06-01 |
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| spelling | doaj.art-a2a1abdad4274060a9be583937ddad552023-06-19T05:32:47ZengDe GruyterOpen Chemistry2391-54202023-06-0121118223210.1515/chem-2022-0338Mineral composition, principal polyphenolic components, and evaluation of the anti-inflammatory, analgesic, and antioxidant properties of Cytisus villosus Pourr leaf extractsZouhri Aziz0El Menyiy Naoual1El-mernissi Yahya2Bouddine Toufik3El-mernissi Rafik4Amhamdi Hassan5Elharrak Abdelhay6Salamatullah Ahmad Mohammad7Nafidi Hiba-Allah8Khallouki Farid9Bourhia Mohammed10Hajji Lhoussain11Bioactives and Environmental Health Laboratory, Faculty of Sciences, Moulay Ismail University, Meknes B.P.11201, MoroccoLaboratory of Pharmacology, National Agency for Medicinal and Aromatic Plants, Taounate34025, MoroccoResearch Unit in Applied Chemistry, Faculty of Science and Techniques, Abdelmalek Essaadi University, Al Hoceima32003, MoroccoBioactives and Environmental Health Laboratory, Faculty of Sciences, Moulay Ismail University, Meknes B.P.11201, MoroccoBioactives and Environmental Health Laboratory, Faculty of Sciences, Moulay Ismail University, Meknes B.P.11201, MoroccoResearch Unit in Applied Chemistry, Faculty of Science and Techniques, Abdelmalek Essaadi University, Al Hoceima32003, MoroccoBioactives and Environmental Health Laboratory, Faculty of Sciences, Moulay Ismail University, Meknes B.P.11201, MoroccoDepartment of Food Science & Nutrition, College of Food and Agricultural Sciences, King Saud University, 11 P.O. Box 2460, Riyadh11451, Saudi ArabiaDepartment of Food Science, Faculty of Agricultural and Food Sciences, Laval University, 2325Quebec City, QC G1V 0A6, CanadaBiology Department, FSTE, University Moulay IsmailBP. 609, 52000, Errachidia, MoroccoLaboratory of Chemistry and Biochemistry, Faculty of Medicine and Pharmacy, Ibn Zohr University, Laayoune70000, MoroccoBioactives and Environmental Health Laboratory, Faculty of Sciences, Moulay Ismail University, Meknes B.P.11201, MoroccoCytisus villosus Pourr. (C. villosus) is a medicinal plant belonging to the Fabaceae family, which grows in the Mediterranean area. It is used in traditional medicine against diseases related to inflammation. The objective of the present study was to identify the mineral and polyphenolic composition as well as to evaluate some biological properties including antioxidant, anti-inflammatory, and analgesic activities of C. villosus leaf aqueous extract. The chemical constituents were identified and quantified using ultra performance liquid chromatography-electrospray ionization tandem mass spectrometry (UPLC-ESI-MS/MS) methods. The antioxidant properties of C. villosus leaves were tested using reducing power (RP), 2,2-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS), and 2,2′-diphenyl-1-picrylhydrazyl (DPPH) assays. The anti-inflammatory potency was evaluated in vitro and in vivo using the albumin denaturation test and the carrageenan test, respectively. Furthermore, the analgesic effect was performed in vivo using tail flick, acetic acid-induced contortion, and plantar tests. Mineralogical analysis revealed that potassium and calcium were the most abundant minerals. The analysis and quantification of the phytochemical composition using UPLC-ESI-MS/MS showed that quinic acid (57.478 ± 1.72 mg/kg) was the major compound of the aqueous extract, followed by salicylic acid (17.38 ± 0.2 mg/kg), isoquercetin (16.895 ± 1.01 mg/kg), and gallic acid (15.914 ± 1.51 mg/kg). The extracts showed potent antioxidant activity for all tests used. The highest antioxidant activity was recorded for the DPPH, ABTS and RP methods, with an IC50 of 3.94 ± 0.09, 2.88 ± 0.07, and 1.94 ± 0.10 μg/mL, respectively. Additionally, using the most frequent analgesic assays, the aqueous extract at a dose of 500 mg/kg exhibited a potent analgesic activity. Notably, an interesting inhibition of albumin denaturation was recorded with an IC50 of 383.94 μg/mL, corroborating the in vivo test. Overall, the results presented here may represent a scientific basis for the traditional use of C. villosus in the treatment of inflammation-related diseases.https://doi.org/10.1515/chem-2022-0338antioxidant activityanti-inflammatoryanalgesic activitycytisus villosusuplc-esi-ms/ms |
| spellingShingle | Zouhri Aziz El Menyiy Naoual El-mernissi Yahya Bouddine Toufik El-mernissi Rafik Amhamdi Hassan Elharrak Abdelhay Salamatullah Ahmad Mohammad Nafidi Hiba-Allah Khallouki Farid Bourhia Mohammed Hajji Lhoussain Mineral composition, principal polyphenolic components, and evaluation of the anti-inflammatory, analgesic, and antioxidant properties of Cytisus villosus Pourr leaf extracts Open Chemistry antioxidant activity anti-inflammatory analgesic activity cytisus villosus uplc-esi-ms/ms |
| title | Mineral composition, principal polyphenolic components, and evaluation of the anti-inflammatory, analgesic, and antioxidant properties of Cytisus villosus Pourr leaf extracts |
| title_full | Mineral composition, principal polyphenolic components, and evaluation of the anti-inflammatory, analgesic, and antioxidant properties of Cytisus villosus Pourr leaf extracts |
| title_fullStr | Mineral composition, principal polyphenolic components, and evaluation of the anti-inflammatory, analgesic, and antioxidant properties of Cytisus villosus Pourr leaf extracts |
| title_full_unstemmed | Mineral composition, principal polyphenolic components, and evaluation of the anti-inflammatory, analgesic, and antioxidant properties of Cytisus villosus Pourr leaf extracts |
| title_short | Mineral composition, principal polyphenolic components, and evaluation of the anti-inflammatory, analgesic, and antioxidant properties of Cytisus villosus Pourr leaf extracts |
| title_sort | mineral composition principal polyphenolic components and evaluation of the anti inflammatory analgesic and antioxidant properties of cytisus villosus pourr leaf extracts |
| topic | antioxidant activity anti-inflammatory analgesic activity cytisus villosus uplc-esi-ms/ms |
| url | https://doi.org/10.1515/chem-2022-0338 |
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