The effect of sodium thiopental as a GABA mimetic drug in neonatal period on expression of GAD65 and GAD67 genes in hippocampus of newborn and adult male rats
Objective(s): Development of the nervous system in human and most animals is continued after the birth. Critical role of this period in generation and specialization of the neuronal circuits is confirmed in numerous studies. Any pharmacological intervention in this period may result in structural, f...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2017-09-01
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| Series: | Iranian Journal of Basic Medical Sciences |
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| Online Access: | http://ijbms.mums.ac.ir/article_9264_d726d25ed460dc8c6e953c67dbf0528c.pdf |
| _version_ | 1818957109920792576 |
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| author | Masoud Naseri Abbas Parham Ali Moghimi |
| author_facet | Masoud Naseri Abbas Parham Ali Moghimi |
| author_sort | Masoud Naseri |
| collection | DOAJ |
| description | Objective(s): Development of the nervous system in human and most animals is continued after the birth. Critical role of this period in generation and specialization of the neuronal circuits is confirmed in numerous studies. Any pharmacological intervention in this period may result in structural, functional or behavioral abnormalities. In this study, sodium thiopental a GABA mimetic drug was administrated to newborn rats and their GAD65 and GAD67 expression in hippocampus was evaluated before and after puberty. Materials and Methods: Newborn male Wistar rats were received sodium thiopental (35 mg/kg) daily for 11 days (from 4 to 14 days after birth). Expression of GAD65 and GAD67 in their hippocampus was compared with control groups in 15 and 45 days after birth with RT-qPCR method. Results: Significant down regulation of GAD65 and GAD67 gene expression was observed in treated rats compared with control group in 45 days after birth animals. But no significant difference was shown between experimental and control groups 15 days after birth animals. Conclusion: The effect of sodium thiopental on GAD65 and GAD67 expression only at adult rats showed a latent period of influence which can be attributed to dosage or intension of sodium thiopental neurotoxicity. Significant down regulation of GAD65 and GAD67 showed unwanted effect of sodium thiopental as GABA mimetic drug in critical period of development. |
| first_indexed | 2024-12-20T11:04:38Z |
| format | Article |
| id | doaj.art-a2a2ccb04e0446d2b1e359e9beb8b4e0 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-12-20T11:04:38Z |
| publishDate | 2017-09-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-a2a2ccb04e0446d2b1e359e9beb8b4e02022-12-21T19:42:55ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742017-09-01209996100110.22038/ijbms.2017.92649264The effect of sodium thiopental as a GABA mimetic drug in neonatal period on expression of GAD65 and GAD67 genes in hippocampus of newborn and adult male ratsMasoud Naseri0Abbas Parham1Ali Moghimi2Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, IranDivision of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Ferdowsi University of Mashhad, Mashhad, Iran|Embryonic and Stem Cell Biology and Biotechnology Research Group, Institute of Biotechnology, Ferdowsi University of Mashhad, Mashhad, IranRayan Center for Neuroscience and Behavior, Department of Biology, Faculty of Science, Ferdowsi University of Mashhad, Mashhad, IranObjective(s): Development of the nervous system in human and most animals is continued after the birth. Critical role of this period in generation and specialization of the neuronal circuits is confirmed in numerous studies. Any pharmacological intervention in this period may result in structural, functional or behavioral abnormalities. In this study, sodium thiopental a GABA mimetic drug was administrated to newborn rats and their GAD65 and GAD67 expression in hippocampus was evaluated before and after puberty. Materials and Methods: Newborn male Wistar rats were received sodium thiopental (35 mg/kg) daily for 11 days (from 4 to 14 days after birth). Expression of GAD65 and GAD67 in their hippocampus was compared with control groups in 15 and 45 days after birth with RT-qPCR method. Results: Significant down regulation of GAD65 and GAD67 gene expression was observed in treated rats compared with control group in 45 days after birth animals. But no significant difference was shown between experimental and control groups 15 days after birth animals. Conclusion: The effect of sodium thiopental on GAD65 and GAD67 expression only at adult rats showed a latent period of influence which can be attributed to dosage or intension of sodium thiopental neurotoxicity. Significant down regulation of GAD65 and GAD67 showed unwanted effect of sodium thiopental as GABA mimetic drug in critical period of development.http://ijbms.mums.ac.ir/article_9264_d726d25ed460dc8c6e953c67dbf0528c.pdfGamma aminobutyric acidGlutamate decarboxylase 1Glutamate decarboxylase 2Real-time polymerase chain reactionThiopental |
| spellingShingle | Masoud Naseri Abbas Parham Ali Moghimi The effect of sodium thiopental as a GABA mimetic drug in neonatal period on expression of GAD65 and GAD67 genes in hippocampus of newborn and adult male rats Iranian Journal of Basic Medical Sciences Gamma aminobutyric acid Glutamate decarboxylase 1 Glutamate decarboxylase 2 Real-time polymerase chain reaction Thiopental |
| title | The effect of sodium thiopental as a GABA mimetic drug in neonatal period on expression of GAD65 and GAD67 genes in hippocampus of newborn and adult male rats |
| title_full | The effect of sodium thiopental as a GABA mimetic drug in neonatal period on expression of GAD65 and GAD67 genes in hippocampus of newborn and adult male rats |
| title_fullStr | The effect of sodium thiopental as a GABA mimetic drug in neonatal period on expression of GAD65 and GAD67 genes in hippocampus of newborn and adult male rats |
| title_full_unstemmed | The effect of sodium thiopental as a GABA mimetic drug in neonatal period on expression of GAD65 and GAD67 genes in hippocampus of newborn and adult male rats |
| title_short | The effect of sodium thiopental as a GABA mimetic drug in neonatal period on expression of GAD65 and GAD67 genes in hippocampus of newborn and adult male rats |
| title_sort | effect of sodium thiopental as a gaba mimetic drug in neonatal period on expression of gad65 and gad67 genes in hippocampus of newborn and adult male rats |
| topic | Gamma aminobutyric acid Glutamate decarboxylase 1 Glutamate decarboxylase 2 Real-time polymerase chain reaction Thiopental |
| url | http://ijbms.mums.ac.ir/article_9264_d726d25ed460dc8c6e953c67dbf0528c.pdf |
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