Effects of Leucosporidium‐derived ice‐binding protein (LeIBP) on bull semen cryopreservation
Abstract We examined the effect of ice‐binding protein derived from Leucosporidium (LeIBP) on the cryopreservation of bull semen and compared it with that derived from previously reported Antifreeze Protein III (AFPIII). Six concentrations of LeIBP (10–1 ~ 104 μg/ml) and AFPIII (10–1 ~ 104 μg/ml) we...
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| Format: | Article |
| Language: | English |
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Wiley
2020-08-01
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| Series: | Veterinary Medicine and Science |
| Subjects: | |
| Online Access: | https://doi.org/10.1002/vms3.269 |
| _version_ | 1828877148846292992 |
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| author | Hoon Jang Hyo J. Kwon Wu S. Sun Seongsoo Hwang In S. Hwang Sungwoo Kim Jun H. Lee Sung G. Lee Jeong W. Lee |
| author_facet | Hoon Jang Hyo J. Kwon Wu S. Sun Seongsoo Hwang In S. Hwang Sungwoo Kim Jun H. Lee Sung G. Lee Jeong W. Lee |
| author_sort | Hoon Jang |
| collection | DOAJ |
| description | Abstract We examined the effect of ice‐binding protein derived from Leucosporidium (LeIBP) on the cryopreservation of bull semen and compared it with that derived from previously reported Antifreeze Protein III (AFPIII). Six concentrations of LeIBP (10–1 ~ 104 μg/ml) and AFPIII (10–1 ~ 104 μg/ml) were added to the bull semen extender, respectively. Sperm kinematic parameters were measured to examine sperm toxicity and cryopreserved sperm quality. Measures of antioxidant activity such as superoxide dismutase (SOD), reduced glutathione/oxidative glutathione (GSH/GSSG), and total antioxidant capacity (TAC) were analysed to identify the effect of LeIBP on sperm quality. In addition, sperm viability was analysed using a flow cytometer and fluorescence microscope by SYBR14/PI staining. The results showed that the LeIBP groups (0.1, 1 and 10 μg/ml) were less toxic, and the quality of the sperm were dramatically improved in the extenders containing 0.1 μg/ml LeIBP among concentrations of LeIBP and AFPIII. The SOD activity of LeIBP was greater than that of AFPIII and control. In addition, sperm viability was enhanced in the LeIBP‐treated group. In summary, LeIBP is a useful cryoprotective adjuvant for bull sperm cryopreservation, and the most efficient concentration of LeIBP is 0.1 μg/ml. |
| first_indexed | 2024-12-13T08:35:07Z |
| format | Article |
| id | doaj.art-a2a3be6beda545eba2af7811f8d74605 |
| institution | Directory Open Access Journal |
| issn | 2053-1095 |
| language | English |
| last_indexed | 2024-12-13T08:35:07Z |
| publishDate | 2020-08-01 |
| publisher | Wiley |
| record_format | Article |
| series | Veterinary Medicine and Science |
| spelling | doaj.art-a2a3be6beda545eba2af7811f8d746052022-12-21T23:53:39ZengWileyVeterinary Medicine and Science2053-10952020-08-016344745310.1002/vms3.269Effects of Leucosporidium‐derived ice‐binding protein (LeIBP) on bull semen cryopreservationHoon Jang0Hyo J. Kwon1Wu S. Sun2Seongsoo Hwang3In S. Hwang4Sungwoo Kim5Jun H. Lee6Sung G. Lee7Jeong W. Lee8Biotherapeutics Translational Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon KoreaBiotherapeutics Translational Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon KoreaBiotherapeutics Translational Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon KoreaAnimal Biotechnology Division National Institute of Animal Science Wanju KoreaAnimal Biotechnology Division National Institute of Animal Science Wanju KoreaAnimal Genetic Resources Station National Institute of Animal Science Namwon KoreaDepartment of Polar Bioconvergence Research Division of life Science Korea Polar Research Institute Incheon KoreaDepartment of Polar Bioconvergence Research Division of life Science Korea Polar Research Institute Incheon KoreaBiotherapeutics Translational Research Center Korea Research Institute of Bioscience and Biotechnology Daejeon KoreaAbstract We examined the effect of ice‐binding protein derived from Leucosporidium (LeIBP) on the cryopreservation of bull semen and compared it with that derived from previously reported Antifreeze Protein III (AFPIII). Six concentrations of LeIBP (10–1 ~ 104 μg/ml) and AFPIII (10–1 ~ 104 μg/ml) were added to the bull semen extender, respectively. Sperm kinematic parameters were measured to examine sperm toxicity and cryopreserved sperm quality. Measures of antioxidant activity such as superoxide dismutase (SOD), reduced glutathione/oxidative glutathione (GSH/GSSG), and total antioxidant capacity (TAC) were analysed to identify the effect of LeIBP on sperm quality. In addition, sperm viability was analysed using a flow cytometer and fluorescence microscope by SYBR14/PI staining. The results showed that the LeIBP groups (0.1, 1 and 10 μg/ml) were less toxic, and the quality of the sperm were dramatically improved in the extenders containing 0.1 μg/ml LeIBP among concentrations of LeIBP and AFPIII. The SOD activity of LeIBP was greater than that of AFPIII and control. In addition, sperm viability was enhanced in the LeIBP‐treated group. In summary, LeIBP is a useful cryoprotective adjuvant for bull sperm cryopreservation, and the most efficient concentration of LeIBP is 0.1 μg/ml.https://doi.org/10.1002/vms3.269Antioxidant activityBull spermCryopreservationLeIBP |
| spellingShingle | Hoon Jang Hyo J. Kwon Wu S. Sun Seongsoo Hwang In S. Hwang Sungwoo Kim Jun H. Lee Sung G. Lee Jeong W. Lee Effects of Leucosporidium‐derived ice‐binding protein (LeIBP) on bull semen cryopreservation Veterinary Medicine and Science Antioxidant activity Bull sperm Cryopreservation LeIBP |
| title | Effects of Leucosporidium‐derived ice‐binding protein (LeIBP) on bull semen cryopreservation |
| title_full | Effects of Leucosporidium‐derived ice‐binding protein (LeIBP) on bull semen cryopreservation |
| title_fullStr | Effects of Leucosporidium‐derived ice‐binding protein (LeIBP) on bull semen cryopreservation |
| title_full_unstemmed | Effects of Leucosporidium‐derived ice‐binding protein (LeIBP) on bull semen cryopreservation |
| title_short | Effects of Leucosporidium‐derived ice‐binding protein (LeIBP) on bull semen cryopreservation |
| title_sort | effects of leucosporidium derived ice binding protein leibp on bull semen cryopreservation |
| topic | Antioxidant activity Bull sperm Cryopreservation LeIBP |
| url | https://doi.org/10.1002/vms3.269 |
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