Antibodies raised against hemolymph of Anopheles culicifacies reduce the fecundity and malaria parasite development
Background & objectives: Several studies have been made to study the effect of antisera raisedagainst different tissues (hemolymh, ovary, midgut and salivary glands) on the fecundity andmalaria parasite development in the different species of mosquitoes but there are no reports on theantisera ra...
| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
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Wolters Kluwer Medknow Publications
2009-12-01
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| Series: | Journal of Vector Borne Diseases |
| Subjects: | |
| Online Access: | http://www.mrcindia.org/journal/issues/464255.pdf |
| _version_ | 1819242601490939904 |
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| author | Amrita Kumari S.K. Gakhar Vikas Hooda |
| author_facet | Amrita Kumari S.K. Gakhar Vikas Hooda |
| author_sort | Amrita Kumari |
| collection | DOAJ |
| description | Background & objectives: Several studies have been made to study the effect of antisera raisedagainst different tissues (hemolymh, ovary, midgut and salivary glands) on the fecundity andmalaria parasite development in the different species of mosquitoes but there are no reports on theantisera raised against the hemolymph of Anopheles culicifacies, the principal malaria vector inIndia accounting for 65% of malaria cases. Hence, an attempt was made to study the same andevaluate its impact on malaria parasite development.Methods: Polyclonal and multifactorial antibodies were produced in rabbits against heterogenousmixture of hemolymph proteins. Antibodies against hemolymph proteins were screened for theirpotential to influence reproductive performance of mosquitoes. Antibody titer in rabbit serum wasdetermined by ELISA and putative candidate antigens were identified in the hemolymph of An.culicifacies by western blotting. Cross reactivity amongst various tissues vis-a-vis hemolymphprotein was also identified. In addition, a significant reduction in oocyst development was alsoobserved in An. culicifacies mosquitoes that ingested antihemolymph antibodies along withPlasmodium vivax.Results: The maximum reduction in fecundity (57%) was observed during fourth week, after thelast booster and number of oocyts per infected mosquito reduced by 73.35% in the group ofmosquitoes that ingested antihemolymph antibodies along with the infected blood meal respectively.However, the ingestion of antibodies against hemolymph proteins did not have significant influenceon hatchability. Antisera raised against hemolymph proteins of An. culicifacies recognized 11polypeptides by western blotting.Interpretation & conclusion: During the present study, 11 putative candidate antigens were identifiedin the hemolymph of An. culicifacies, against which antibodies produced significantly reduced thefecundity by 57%. In addition, a significant reduction in oocyst development was also observed inAn. culicifacies that ingested antihemolymph antibodies along with P.vivax. |
| first_indexed | 2024-12-23T14:42:24Z |
| format | Article |
| id | doaj.art-a2a436e4437844888f86b5b007a19c82 |
| institution | Directory Open Access Journal |
| issn | 0972-9062 |
| language | English |
| last_indexed | 2024-12-23T14:42:24Z |
| publishDate | 2009-12-01 |
| publisher | Wolters Kluwer Medknow Publications |
| record_format | Article |
| series | Journal of Vector Borne Diseases |
| spelling | doaj.art-a2a436e4437844888f86b5b007a19c822022-12-21T17:43:10ZengWolters Kluwer Medknow PublicationsJournal of Vector Borne Diseases0972-90622009-12-01464255260Antibodies raised against hemolymph of Anopheles culicifacies reduce the fecundity and malaria parasite developmentAmrita KumariS.K. GakharVikas HoodaBackground & objectives: Several studies have been made to study the effect of antisera raisedagainst different tissues (hemolymh, ovary, midgut and salivary glands) on the fecundity andmalaria parasite development in the different species of mosquitoes but there are no reports on theantisera raised against the hemolymph of Anopheles culicifacies, the principal malaria vector inIndia accounting for 65% of malaria cases. Hence, an attempt was made to study the same andevaluate its impact on malaria parasite development.Methods: Polyclonal and multifactorial antibodies were produced in rabbits against heterogenousmixture of hemolymph proteins. Antibodies against hemolymph proteins were screened for theirpotential to influence reproductive performance of mosquitoes. Antibody titer in rabbit serum wasdetermined by ELISA and putative candidate antigens were identified in the hemolymph of An.culicifacies by western blotting. Cross reactivity amongst various tissues vis-a-vis hemolymphprotein was also identified. In addition, a significant reduction in oocyst development was alsoobserved in An. culicifacies mosquitoes that ingested antihemolymph antibodies along withPlasmodium vivax.Results: The maximum reduction in fecundity (57%) was observed during fourth week, after thelast booster and number of oocyts per infected mosquito reduced by 73.35% in the group ofmosquitoes that ingested antihemolymph antibodies along with the infected blood meal respectively.However, the ingestion of antibodies against hemolymph proteins did not have significant influenceon hatchability. Antisera raised against hemolymph proteins of An. culicifacies recognized 11polypeptides by western blotting.Interpretation & conclusion: During the present study, 11 putative candidate antigens were identifiedin the hemolymph of An. culicifacies, against which antibodies produced significantly reduced thefecundity by 57%. In addition, a significant reduction in oocyst development was also observed inAn. culicifacies that ingested antihemolymph antibodies along with P.vivax.http://www.mrcindia.org/journal/issues/464255.pdfAnopheles culicifaciesantibodieshemolymphmalaria parasitetransmissionvaccine |
| spellingShingle | Amrita Kumari S.K. Gakhar Vikas Hooda Antibodies raised against hemolymph of Anopheles culicifacies reduce the fecundity and malaria parasite development Journal of Vector Borne Diseases Anopheles culicifacies antibodies hemolymph malaria parasite transmission vaccine |
| title | Antibodies raised against hemolymph of Anopheles culicifacies reduce the fecundity and malaria parasite development |
| title_full | Antibodies raised against hemolymph of Anopheles culicifacies reduce the fecundity and malaria parasite development |
| title_fullStr | Antibodies raised against hemolymph of Anopheles culicifacies reduce the fecundity and malaria parasite development |
| title_full_unstemmed | Antibodies raised against hemolymph of Anopheles culicifacies reduce the fecundity and malaria parasite development |
| title_short | Antibodies raised against hemolymph of Anopheles culicifacies reduce the fecundity and malaria parasite development |
| title_sort | antibodies raised against hemolymph of anopheles culicifacies reduce the fecundity and malaria parasite development |
| topic | Anopheles culicifacies antibodies hemolymph malaria parasite transmission vaccine |
| url | http://www.mrcindia.org/journal/issues/464255.pdf |
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