Dielectrophoretic Micro-Organization of Chondrocytes to Regenerate Mechanically Anisotropic Cartilaginous Tissue
Recently, many studies have focused on the repair and regeneration of damaged articular cartilage using tissue engineering. In tissue engineering therapy, cells are cultured in vitro to create a three-dimensional (3-D) tissue designed to replace the damaged cartilage. Although tissue engineering is...
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| Format: | Article |
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MDPI AG
2021-09-01
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| Series: | Micromachines |
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| Online Access: | https://www.mdpi.com/2072-666X/12/9/1098 |
| _version_ | 1797518164715634688 |
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| author | Yoshitaka Takeuchi Shogo Miyata |
| author_facet | Yoshitaka Takeuchi Shogo Miyata |
| author_sort | Yoshitaka Takeuchi |
| collection | DOAJ |
| description | Recently, many studies have focused on the repair and regeneration of damaged articular cartilage using tissue engineering. In tissue engineering therapy, cells are cultured in vitro to create a three-dimensional (3-D) tissue designed to replace the damaged cartilage. Although tissue engineering is a useful approach to regenerating cartilage, mechanical anisotropy has not been reconstructed from a cellular organization level. This study aims to create mechanically anisotropic cartilaginous tissue using dielectrophoretic cell patterning and gel-sheet lamination. Bovine chondrocytes were patterned in a hydrogel to form line-array cell clusters via negative dielectrophoresis (DEP). The results indicate that the embedded chondrocytes remained viable and reconstructed cartilaginous tissue along the patterned cell array. Moreover, the agarose gel, in which chondrocytes were patterned, demonstrated mechanical anisotropy. In summary, our DEP cell patterning and gel-sheet lamination techniques would be useful for reconstructing mechanically anisotropic cartilage tissues. |
| first_indexed | 2024-03-10T07:26:18Z |
| format | Article |
| id | doaj.art-a2a4cd7041a8491c957c353fa4f17185 |
| institution | Directory Open Access Journal |
| issn | 2072-666X |
| language | English |
| last_indexed | 2024-03-10T07:26:18Z |
| publishDate | 2021-09-01 |
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| record_format | Article |
| series | Micromachines |
| spelling | doaj.art-a2a4cd7041a8491c957c353fa4f171852023-11-22T14:16:34ZengMDPI AGMicromachines2072-666X2021-09-01129109810.3390/mi12091098Dielectrophoretic Micro-Organization of Chondrocytes to Regenerate Mechanically Anisotropic Cartilaginous TissueYoshitaka Takeuchi0Shogo Miyata1Graduate School of Science and Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, JapanFaculty of Science & Technology, Keio University, 3-14-1 Hiyoshi, Kohoku-ku, Yokohama 223-8522, JapanRecently, many studies have focused on the repair and regeneration of damaged articular cartilage using tissue engineering. In tissue engineering therapy, cells are cultured in vitro to create a three-dimensional (3-D) tissue designed to replace the damaged cartilage. Although tissue engineering is a useful approach to regenerating cartilage, mechanical anisotropy has not been reconstructed from a cellular organization level. This study aims to create mechanically anisotropic cartilaginous tissue using dielectrophoretic cell patterning and gel-sheet lamination. Bovine chondrocytes were patterned in a hydrogel to form line-array cell clusters via negative dielectrophoresis (DEP). The results indicate that the embedded chondrocytes remained viable and reconstructed cartilaginous tissue along the patterned cell array. Moreover, the agarose gel, in which chondrocytes were patterned, demonstrated mechanical anisotropy. In summary, our DEP cell patterning and gel-sheet lamination techniques would be useful for reconstructing mechanically anisotropic cartilage tissues.https://www.mdpi.com/2072-666X/12/9/1098dielectrophoresischondrocytemechanical anisotropytissue engineeringcell patterning |
| spellingShingle | Yoshitaka Takeuchi Shogo Miyata Dielectrophoretic Micro-Organization of Chondrocytes to Regenerate Mechanically Anisotropic Cartilaginous Tissue Micromachines dielectrophoresis chondrocyte mechanical anisotropy tissue engineering cell patterning |
| title | Dielectrophoretic Micro-Organization of Chondrocytes to Regenerate Mechanically Anisotropic Cartilaginous Tissue |
| title_full | Dielectrophoretic Micro-Organization of Chondrocytes to Regenerate Mechanically Anisotropic Cartilaginous Tissue |
| title_fullStr | Dielectrophoretic Micro-Organization of Chondrocytes to Regenerate Mechanically Anisotropic Cartilaginous Tissue |
| title_full_unstemmed | Dielectrophoretic Micro-Organization of Chondrocytes to Regenerate Mechanically Anisotropic Cartilaginous Tissue |
| title_short | Dielectrophoretic Micro-Organization of Chondrocytes to Regenerate Mechanically Anisotropic Cartilaginous Tissue |
| title_sort | dielectrophoretic micro organization of chondrocytes to regenerate mechanically anisotropic cartilaginous tissue |
| topic | dielectrophoresis chondrocyte mechanical anisotropy tissue engineering cell patterning |
| url | https://www.mdpi.com/2072-666X/12/9/1098 |
| work_keys_str_mv | AT yoshitakatakeuchi dielectrophoreticmicroorganizationofchondrocytestoregeneratemechanicallyanisotropiccartilaginoustissue AT shogomiyata dielectrophoreticmicroorganizationofchondrocytestoregeneratemechanicallyanisotropiccartilaginoustissue |