Luteolin Reduced the Traumatic Brain Injury-Induced Memory Impairments in Rats: Attenuating Oxidative Stress and Dark Neurons of Hippocampus
Traumatic Brain Injury (TBI) is generally recognized as a major risk factor for memory impairments and Alzheimer’s disease (AD). In this experimental study, our aim was to investigate the ameliorating effects of luteolin (LUT) on the memory impairments, oxidative stress, and histopathological change...
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| Format: | Article |
| Language: | English |
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Tehran University of Medical Sciences
2018-12-01
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| Series: | Acta Medica Iranica |
| Subjects: | |
| Online Access: | https://acta.tums.ac.ir/index.php/acta/article/view/7437 |
| _version_ | 1818039651251781632 |
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| author | Zeinab Ashaari Gholamreza Hassanzadeh Tahmineh Mokhtari Mahmoud Hosseini Zakieh Keshavarzi Mohsen Amini Kowsar Bavarsad Sahar Ijaz Mousa-Al-Reza Hadjzadeh |
| author_facet | Zeinab Ashaari Gholamreza Hassanzadeh Tahmineh Mokhtari Mahmoud Hosseini Zakieh Keshavarzi Mohsen Amini Kowsar Bavarsad Sahar Ijaz Mousa-Al-Reza Hadjzadeh |
| author_sort | Zeinab Ashaari |
| collection | DOAJ |
| description | Traumatic Brain Injury (TBI) is generally recognized as a major risk factor for memory impairments and Alzheimer’s disease (AD). In this experimental study, our aim was to investigate the ameliorating effects of luteolin (LUT) on the memory impairments, oxidative stress, and histopathological changes induced by TBI in rats. The adult male Wistar rats were randomly divided into six groups including: Control (Co), sham, TBI, TBI+LUT (10 mg/kg), TBI +LUT (25 mg/kg), TBI +LUT (50 mg/kg). To evaluate the protective effects of LUT on the memory of the rats, passive avoidance test using shuttle box was performed. Finally, the animals were anesthetized, and the brain tissues were removed and analyzed for oxidative stress parameters. Using histological methods, dark neuron production was also evaluated. There was a significant decrease in the latency time to enter the dark compartment in passive avoidance test in TBI animals. This latency time was significantly increased in TBI+LUT (25 mg/kg) and TBI+LUT (50 mg/kg) groups along with significant increases in superoxide dismutase and catalase activity in the hippocampal zone and a decrease in malondialdehyde (MDA). The number of dark neurons in the hippocampus decreased with all three doses of LUT. In the present study, LUT showed neuroprotective effects, improvement in learning and reduction in memory impairment induced by TBI in rats. Protection against oxidative stress might be a possible mechanism behind these effects. Further works are necessary to work out if LUT is potentially a suitable therapeutic candidate for neural disorders. |
| first_indexed | 2024-12-10T08:02:01Z |
| format | Article |
| id | doaj.art-a2ac7e9c037c49f58e7f4d96ca59c4f1 |
| institution | Directory Open Access Journal |
| issn | 0044-6025 1735-9694 |
| language | English |
| last_indexed | 2024-12-10T08:02:01Z |
| publishDate | 2018-12-01 |
| publisher | Tehran University of Medical Sciences |
| record_format | Article |
| series | Acta Medica Iranica |
| spelling | doaj.art-a2ac7e9c037c49f58e7f4d96ca59c4f12022-12-22T01:56:45ZengTehran University of Medical SciencesActa Medica Iranica0044-60251735-96942018-12-015697437Luteolin Reduced the Traumatic Brain Injury-Induced Memory Impairments in Rats: Attenuating Oxidative Stress and Dark Neurons of HippocampusZeinab Ashaari0Gholamreza Hassanzadeh1Tahmineh Mokhtari2Mahmoud Hosseini3Zakieh Keshavarzi4Mohsen Amini5Kowsar Bavarsad6Sahar Ijaz7Mousa-Al-Reza Hadjzadeh8Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran. AND Department of Neuroscience, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran.Nervous System Stem Cells Research Center, Semnan University of Medical Sciences, Semnan, Iran. AND Department of Anatomy, School of Medicine, Semnan University of Medical Sciences, Semnan, Iran.Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Natural Products and Medicinal Plants Research Center, North Khorasan University of Medical Sciences, Bojnurd, Iran.Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran.Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.Department of Physiology, School of Medicine, Mashhad University of Medical Sciences, Mashhad, Iran. AND Department of Neurocognitive Sciences, Psychiatry and Behavioral Sciences Research Center, Mashhad University of Medical Sciences, Mashhad, Iran. AND Neurogenic Inflammation Research Center, Mashhad University of Medical Sciences, Mashhad, Iran.Traumatic Brain Injury (TBI) is generally recognized as a major risk factor for memory impairments and Alzheimer’s disease (AD). In this experimental study, our aim was to investigate the ameliorating effects of luteolin (LUT) on the memory impairments, oxidative stress, and histopathological changes induced by TBI in rats. The adult male Wistar rats were randomly divided into six groups including: Control (Co), sham, TBI, TBI+LUT (10 mg/kg), TBI +LUT (25 mg/kg), TBI +LUT (50 mg/kg). To evaluate the protective effects of LUT on the memory of the rats, passive avoidance test using shuttle box was performed. Finally, the animals were anesthetized, and the brain tissues were removed and analyzed for oxidative stress parameters. Using histological methods, dark neuron production was also evaluated. There was a significant decrease in the latency time to enter the dark compartment in passive avoidance test in TBI animals. This latency time was significantly increased in TBI+LUT (25 mg/kg) and TBI+LUT (50 mg/kg) groups along with significant increases in superoxide dismutase and catalase activity in the hippocampal zone and a decrease in malondialdehyde (MDA). The number of dark neurons in the hippocampus decreased with all three doses of LUT. In the present study, LUT showed neuroprotective effects, improvement in learning and reduction in memory impairment induced by TBI in rats. Protection against oxidative stress might be a possible mechanism behind these effects. Further works are necessary to work out if LUT is potentially a suitable therapeutic candidate for neural disorders.https://acta.tums.ac.ir/index.php/acta/article/view/7437LuteolinTraumatic brain injuryOxidative stressLearning and memoryRat |
| spellingShingle | Zeinab Ashaari Gholamreza Hassanzadeh Tahmineh Mokhtari Mahmoud Hosseini Zakieh Keshavarzi Mohsen Amini Kowsar Bavarsad Sahar Ijaz Mousa-Al-Reza Hadjzadeh Luteolin Reduced the Traumatic Brain Injury-Induced Memory Impairments in Rats: Attenuating Oxidative Stress and Dark Neurons of Hippocampus Acta Medica Iranica Luteolin Traumatic brain injury Oxidative stress Learning and memory Rat |
| title | Luteolin Reduced the Traumatic Brain Injury-Induced Memory Impairments in Rats: Attenuating Oxidative Stress and Dark Neurons of Hippocampus |
| title_full | Luteolin Reduced the Traumatic Brain Injury-Induced Memory Impairments in Rats: Attenuating Oxidative Stress and Dark Neurons of Hippocampus |
| title_fullStr | Luteolin Reduced the Traumatic Brain Injury-Induced Memory Impairments in Rats: Attenuating Oxidative Stress and Dark Neurons of Hippocampus |
| title_full_unstemmed | Luteolin Reduced the Traumatic Brain Injury-Induced Memory Impairments in Rats: Attenuating Oxidative Stress and Dark Neurons of Hippocampus |
| title_short | Luteolin Reduced the Traumatic Brain Injury-Induced Memory Impairments in Rats: Attenuating Oxidative Stress and Dark Neurons of Hippocampus |
| title_sort | luteolin reduced the traumatic brain injury induced memory impairments in rats attenuating oxidative stress and dark neurons of hippocampus |
| topic | Luteolin Traumatic brain injury Oxidative stress Learning and memory Rat |
| url | https://acta.tums.ac.ir/index.php/acta/article/view/7437 |
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