Gold immunochromatographic assay for kitasamycin and josamycin residues screening in milk and egg samples
In this study, a kitasamycin (KIT) hapten was prepared through the o-carboxymethoxylamine method and used to produce a monoclonal antibody against KIT and josamycin (JOS). The half inhibition concentration for an indirect competitive enzyme-linked immunosorbent assay was 1.49 and 2.67 ng/mL, respect...
| Main Authors: | , , , , , |
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2019-01-01
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| Series: | Food and Agricultural Immunology |
| Subjects: | |
| Online Access: | http://dx.doi.org/10.1080/09540105.2019.1677567 |
| _version_ | 1818279954942525440 |
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| author | Lingling Guo Liqiang Liu Gang Cui Shufeng Ma Xiaoling Wu Hua Kuang |
| author_facet | Lingling Guo Liqiang Liu Gang Cui Shufeng Ma Xiaoling Wu Hua Kuang |
| author_sort | Lingling Guo |
| collection | DOAJ |
| description | In this study, a kitasamycin (KIT) hapten was prepared through the o-carboxymethoxylamine method and used to produce a monoclonal antibody against KIT and josamycin (JOS). The half inhibition concentration for an indirect competitive enzyme-linked immunosorbent assay was 1.49 and 2.67 ng/mL, respectively. Based on this antibody, a gold immunochromatographic assay (GICA) was developed for the detection of KIT and JOS residues in milk and egg samples. A hand-held strip scanner was used for quantitative analysis, with limits of detection for KIT and JOS of 1.51 and 1.91 μg/L in milk, and 3.0 and 2.72 μg/kg in eggs. The proposed GICA was validated by LC–MS/MS, which indicated that the GICA method would be a useful tool for the on-site detection of KIT and JOS residues in milk and egg samples. |
| first_indexed | 2024-12-12T23:41:33Z |
| format | Article |
| id | doaj.art-a2b2767ab66e4bec8363a41dfee45d07 |
| institution | Directory Open Access Journal |
| issn | 0954-0105 1465-3443 |
| language | English |
| last_indexed | 2024-12-12T23:41:33Z |
| publishDate | 2019-01-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | Food and Agricultural Immunology |
| spelling | doaj.art-a2b2767ab66e4bec8363a41dfee45d072022-12-22T00:07:06ZengTaylor & Francis GroupFood and Agricultural Immunology0954-01051465-34432019-01-013011189120110.1080/09540105.2019.16775671677567Gold immunochromatographic assay for kitasamycin and josamycin residues screening in milk and egg samplesLingling Guo0Liqiang Liu1Gang Cui2Shufeng Ma3Xiaoling Wu4Hua Kuang5Jiangnan UniversityJiangnan UniversityYancheng Teachers UniversityJiangnan UniversityJiangnan UniversityJiangnan UniversityIn this study, a kitasamycin (KIT) hapten was prepared through the o-carboxymethoxylamine method and used to produce a monoclonal antibody against KIT and josamycin (JOS). The half inhibition concentration for an indirect competitive enzyme-linked immunosorbent assay was 1.49 and 2.67 ng/mL, respectively. Based on this antibody, a gold immunochromatographic assay (GICA) was developed for the detection of KIT and JOS residues in milk and egg samples. A hand-held strip scanner was used for quantitative analysis, with limits of detection for KIT and JOS of 1.51 and 1.91 μg/L in milk, and 3.0 and 2.72 μg/kg in eggs. The proposed GICA was validated by LC–MS/MS, which indicated that the GICA method would be a useful tool for the on-site detection of KIT and JOS residues in milk and egg samples.http://dx.doi.org/10.1080/09540105.2019.1677567kitasamycinjosamycingold immunochromatographic assaymonoclonal antibody |
| spellingShingle | Lingling Guo Liqiang Liu Gang Cui Shufeng Ma Xiaoling Wu Hua Kuang Gold immunochromatographic assay for kitasamycin and josamycin residues screening in milk and egg samples Food and Agricultural Immunology kitasamycin josamycin gold immunochromatographic assay monoclonal antibody |
| title | Gold immunochromatographic assay for kitasamycin and josamycin residues screening in milk and egg samples |
| title_full | Gold immunochromatographic assay for kitasamycin and josamycin residues screening in milk and egg samples |
| title_fullStr | Gold immunochromatographic assay for kitasamycin and josamycin residues screening in milk and egg samples |
| title_full_unstemmed | Gold immunochromatographic assay for kitasamycin and josamycin residues screening in milk and egg samples |
| title_short | Gold immunochromatographic assay for kitasamycin and josamycin residues screening in milk and egg samples |
| title_sort | gold immunochromatographic assay for kitasamycin and josamycin residues screening in milk and egg samples |
| topic | kitasamycin josamycin gold immunochromatographic assay monoclonal antibody |
| url | http://dx.doi.org/10.1080/09540105.2019.1677567 |
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