Excavating bioactivities of nanozyme to remodel microenvironment for protecting chondrocytes and delaying osteoarthritis
Osteoarthritis (OA) is the main cause of disability in the elderly. Effective intervention in the early and middle stage of osteoarthritis can greatly prevent or slow down the development of the disease, and reduce the probability of joint replacement. However, there is to date no effective interven...
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| Format: | Article |
| Language: | English |
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KeAi Communications Co., Ltd.
2021-08-01
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| Series: | Bioactive Materials |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S2452199X21000281 |
| _version_ | 1797203178871062528 |
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| author | Weiduo Hou Chenyi Ye Mo Chen Wei Gao Xue Xie Jianrong Wu Kai Zhang Wei Zhang Yuanyi Zheng Xiaojun Cai |
| author_facet | Weiduo Hou Chenyi Ye Mo Chen Wei Gao Xue Xie Jianrong Wu Kai Zhang Wei Zhang Yuanyi Zheng Xiaojun Cai |
| author_sort | Weiduo Hou |
| collection | DOAJ |
| description | Osteoarthritis (OA) is the main cause of disability in the elderly. Effective intervention in the early and middle stage of osteoarthritis can greatly prevent or slow down the development of the disease, and reduce the probability of joint replacement. However, there is to date no effective intervention for early and middle-stage OA. OA microenvironment mainly destroys the balance of oxidative stress, extracellular matrix synthesis and degradation of chondrocytes under the joint action of biological and mechanical factors. Herein, hollow Prussian blue nanozymes (HPBzymes) were designed via a modified hydrothermal template-free method. The aim of this study was to investigate the effects of HPBzymes on chondrocytes and the progression of OA. The intrinsic bioactivities of HPBzymes were excavated in vitro and in vivo, remodeling microenvironment for significantly protecting chondrocytes and delaying the progression of traumatic OA by inhibiting reactive oxygen species (ROS) and Rac1/nuclear factor kappa-B (NF-κB) signaling in a rat model. HPBzyme significantly diminished interleukin (IL)-1β-stimulated inflammation, extracellular matrix degradation, and apoptosis of human chondrocytes. HPBzyme attenuated the expression of Rac1 and the ROS levels and prevented the release and nuclear translocation of NF-κB. Deeply digging the intrinsic bioactivities of nanozyme with single component to remodel microenvironment is an effective strategy for ROS-associated chronic diseases. This study reveals that excavating the bioactivities of nanomedicine deserves attention for diagnosis and treatment of severe diseases. |
| first_indexed | 2024-04-24T08:15:13Z |
| format | Article |
| id | doaj.art-a2b356fd9f544630b0c16a0de72e2375 |
| institution | Directory Open Access Journal |
| issn | 2452-199X |
| language | English |
| last_indexed | 2024-04-24T08:15:13Z |
| publishDate | 2021-08-01 |
| publisher | KeAi Communications Co., Ltd. |
| record_format | Article |
| series | Bioactive Materials |
| spelling | doaj.art-a2b356fd9f544630b0c16a0de72e23752024-04-17T04:00:06ZengKeAi Communications Co., Ltd.Bioactive Materials2452-199X2021-08-016824392451Excavating bioactivities of nanozyme to remodel microenvironment for protecting chondrocytes and delaying osteoarthritisWeiduo Hou0Chenyi Ye1Mo Chen2Wei Gao3Xue Xie4Jianrong Wu5Kai Zhang6Wei Zhang7Yuanyi Zheng8Xiaojun Cai9Department of Orthopaedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, 310009, Hangzhou, China; Research Institute of Orthopaedics, Zhejiang University, 310009, Hangzhou, ChinaDepartment of Orthopaedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, 310009, Hangzhou, China; Research Institute of Orthopaedics, Zhejiang University, 310009, Hangzhou, ChinaDepartment of Rheumatology, Second Affiliated Hospital, School of Medicine, Zhejiang University, 310009, Hangzhou, ChinaDepartment of Ultrasound in Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR ChinaDepartment of Ultrasound in Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR ChinaShanghai Institute of Ultrasound in Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR ChinaLaboratory for Pathophysiological and Health Science, RIKEN Center for Biosystems Dynamics Research, Kobe, Hyogo, 650-0047, JapanDepartment of Orthopaedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, 310009, Hangzhou, China; Research Institute of Orthopaedics, Zhejiang University, 310009, Hangzhou, China; Corresponding author. Department of Orthopaedics, Second Affiliated Hospital, School of Medicine, Zhejiang University, 310009, Hangzhou, China.Department of Ultrasound in Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR China; Corresponding author.Shanghai Institute of Ultrasound in Medicine, Shanghai Jiao Tong University Affiliated Sixth People's Hospital, Shanghai, 200233, PR China; Corresponding author.Osteoarthritis (OA) is the main cause of disability in the elderly. Effective intervention in the early and middle stage of osteoarthritis can greatly prevent or slow down the development of the disease, and reduce the probability of joint replacement. However, there is to date no effective intervention for early and middle-stage OA. OA microenvironment mainly destroys the balance of oxidative stress, extracellular matrix synthesis and degradation of chondrocytes under the joint action of biological and mechanical factors. Herein, hollow Prussian blue nanozymes (HPBzymes) were designed via a modified hydrothermal template-free method. The aim of this study was to investigate the effects of HPBzymes on chondrocytes and the progression of OA. The intrinsic bioactivities of HPBzymes were excavated in vitro and in vivo, remodeling microenvironment for significantly protecting chondrocytes and delaying the progression of traumatic OA by inhibiting reactive oxygen species (ROS) and Rac1/nuclear factor kappa-B (NF-κB) signaling in a rat model. HPBzyme significantly diminished interleukin (IL)-1β-stimulated inflammation, extracellular matrix degradation, and apoptosis of human chondrocytes. HPBzyme attenuated the expression of Rac1 and the ROS levels and prevented the release and nuclear translocation of NF-κB. Deeply digging the intrinsic bioactivities of nanozyme with single component to remodel microenvironment is an effective strategy for ROS-associated chronic diseases. This study reveals that excavating the bioactivities of nanomedicine deserves attention for diagnosis and treatment of severe diseases.http://www.sciencedirect.com/science/article/pii/S2452199X21000281Reactive oxygen speciesPrussian blue nanozymeArthritisInflammationChondrocytes |
| spellingShingle | Weiduo Hou Chenyi Ye Mo Chen Wei Gao Xue Xie Jianrong Wu Kai Zhang Wei Zhang Yuanyi Zheng Xiaojun Cai Excavating bioactivities of nanozyme to remodel microenvironment for protecting chondrocytes and delaying osteoarthritis Bioactive Materials Reactive oxygen species Prussian blue nanozyme Arthritis Inflammation Chondrocytes |
| title | Excavating bioactivities of nanozyme to remodel microenvironment for protecting chondrocytes and delaying osteoarthritis |
| title_full | Excavating bioactivities of nanozyme to remodel microenvironment for protecting chondrocytes and delaying osteoarthritis |
| title_fullStr | Excavating bioactivities of nanozyme to remodel microenvironment for protecting chondrocytes and delaying osteoarthritis |
| title_full_unstemmed | Excavating bioactivities of nanozyme to remodel microenvironment for protecting chondrocytes and delaying osteoarthritis |
| title_short | Excavating bioactivities of nanozyme to remodel microenvironment for protecting chondrocytes and delaying osteoarthritis |
| title_sort | excavating bioactivities of nanozyme to remodel microenvironment for protecting chondrocytes and delaying osteoarthritis |
| topic | Reactive oxygen species Prussian blue nanozyme Arthritis Inflammation Chondrocytes |
| url | http://www.sciencedirect.com/science/article/pii/S2452199X21000281 |
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