The mediatory role of Majie cataplasm on inflammation of allergic asthma through transcription factors related to Th1 and Th2
Abstract Background Asthma, a common respiratory disease, is harmful biological effect to our health. As a traditional Chinese medicine for asthma, Majie cataplasm could alleviate the symptoms of asthma and its compositions have immunomodulatory effects. Previous experiments showed that Majie catapl...
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2020-05-01
|
| Series: | Chinese Medicine |
| Subjects: | |
| Online Access: | http://link.springer.com/article/10.1186/s13020-020-00334-w |
| _version_ | 1818311685816975360 |
|---|---|
| author | Wenting Ji Qianyi Zhang Hanfen Shi Ruijuan Dong Dongyu Ge Xin Du Beida Ren Xueqian Wang Qingguo Wang |
| author_facet | Wenting Ji Qianyi Zhang Hanfen Shi Ruijuan Dong Dongyu Ge Xin Du Beida Ren Xueqian Wang Qingguo Wang |
| author_sort | Wenting Ji |
| collection | DOAJ |
| description | Abstract Background Asthma, a common respiratory disease, is harmful biological effect to our health. As a traditional Chinese medicine for asthma, Majie cataplasm could alleviate the symptoms of asthma and its compositions have immunomodulatory effects. Previous experiments showed that Majie cataplasm was an effective approach to mitigate asthma airway remodeling and had the potential to regulate Th2 cytokines of IL-5 and IL-13. Therefore, our further research focuses on the explanation about the regulatory effect of Majie cataplasm on reshaping Th1/Th2 through their related transcription factors. Methods In this experiment, the launch of asthma model was made by inducing with Ovalbumin (OVA) in C57 mice (n = 40), including 4 groups: the untreated control group (n = 10), the asthma model group (n = 10), the dexamethasone group (n = 10) and the Majie cataplasm group (n = 10). After the intervention, all groups of animals got detected for serum IgE levels, and HE staining of lung tissues was to observe and examine pathological changes. Meanwhile, we analyzed the secretion of IL-4+ T cells and IFN-γ+ T cells in spleen by flow cytometry. The expressions of transcription factor STAT6 mRNA, GATA-3 mRNA and T-bet mRNA in lung tissues was tested by PCR, and western blot had been used to detect levels of JAK2 and STAT3. Results We found that Majie cataplasm eased the content of serum IgE and lung inflammation. It could lower the increased number of IL-4+ T cells and IFN-γ+ T cells (P < 0.0001, P < 0.01) in asthmatic mice and curb the expression of STAT6 mRNA and GATA-3 (P < 0.0001, P < 0.01) mRNA as well as the protein levels of JAK2 (P < 0.001) and the ratio of pSTAT3/STAT3 (P < 0.05). Besides, Majie cataplasm made its mark on T-bet mRNA by improving it (P < 0.0001). Conclusion These data suggest that Majie cataplasm exert an anti-inflammatory effect of Th2 by rebalancing Th1/Th2 through corresponding transcription factor STAT6, GATA-3, STAT3, and T-bet, which providing a strong cornerstone for asthma control. |
| first_indexed | 2024-12-13T08:05:53Z |
| format | Article |
| id | doaj.art-a2b39db414314ebc868ea0c17859cf59 |
| institution | Directory Open Access Journal |
| issn | 1749-8546 |
| language | English |
| last_indexed | 2024-12-13T08:05:53Z |
| publishDate | 2020-05-01 |
| publisher | BMC |
| record_format | Article |
| series | Chinese Medicine |
| spelling | doaj.art-a2b39db414314ebc868ea0c17859cf592022-12-21T23:54:18ZengBMCChinese Medicine1749-85462020-05-0115111010.1186/s13020-020-00334-wThe mediatory role of Majie cataplasm on inflammation of allergic asthma through transcription factors related to Th1 and Th2Wenting Ji0Qianyi Zhang1Hanfen Shi2Ruijuan Dong3Dongyu Ge4Xin Du5Beida Ren6Xueqian Wang7Qingguo Wang8Beijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineBeijing University of Chinese MedicineAbstract Background Asthma, a common respiratory disease, is harmful biological effect to our health. As a traditional Chinese medicine for asthma, Majie cataplasm could alleviate the symptoms of asthma and its compositions have immunomodulatory effects. Previous experiments showed that Majie cataplasm was an effective approach to mitigate asthma airway remodeling and had the potential to regulate Th2 cytokines of IL-5 and IL-13. Therefore, our further research focuses on the explanation about the regulatory effect of Majie cataplasm on reshaping Th1/Th2 through their related transcription factors. Methods In this experiment, the launch of asthma model was made by inducing with Ovalbumin (OVA) in C57 mice (n = 40), including 4 groups: the untreated control group (n = 10), the asthma model group (n = 10), the dexamethasone group (n = 10) and the Majie cataplasm group (n = 10). After the intervention, all groups of animals got detected for serum IgE levels, and HE staining of lung tissues was to observe and examine pathological changes. Meanwhile, we analyzed the secretion of IL-4+ T cells and IFN-γ+ T cells in spleen by flow cytometry. The expressions of transcription factor STAT6 mRNA, GATA-3 mRNA and T-bet mRNA in lung tissues was tested by PCR, and western blot had been used to detect levels of JAK2 and STAT3. Results We found that Majie cataplasm eased the content of serum IgE and lung inflammation. It could lower the increased number of IL-4+ T cells and IFN-γ+ T cells (P < 0.0001, P < 0.01) in asthmatic mice and curb the expression of STAT6 mRNA and GATA-3 (P < 0.0001, P < 0.01) mRNA as well as the protein levels of JAK2 (P < 0.001) and the ratio of pSTAT3/STAT3 (P < 0.05). Besides, Majie cataplasm made its mark on T-bet mRNA by improving it (P < 0.0001). Conclusion These data suggest that Majie cataplasm exert an anti-inflammatory effect of Th2 by rebalancing Th1/Th2 through corresponding transcription factor STAT6, GATA-3, STAT3, and T-bet, which providing a strong cornerstone for asthma control.http://link.springer.com/article/10.1186/s13020-020-00334-wMajie cataplasmTh2 inflammation of allergic asthmaTh1/Th2STAT6GATA-3STAT3 |
| spellingShingle | Wenting Ji Qianyi Zhang Hanfen Shi Ruijuan Dong Dongyu Ge Xin Du Beida Ren Xueqian Wang Qingguo Wang The mediatory role of Majie cataplasm on inflammation of allergic asthma through transcription factors related to Th1 and Th2 Chinese Medicine Majie cataplasm Th2 inflammation of allergic asthma Th1/Th2 STAT6 GATA-3 STAT3 |
| title | The mediatory role of Majie cataplasm on inflammation of allergic asthma through transcription factors related to Th1 and Th2 |
| title_full | The mediatory role of Majie cataplasm on inflammation of allergic asthma through transcription factors related to Th1 and Th2 |
| title_fullStr | The mediatory role of Majie cataplasm on inflammation of allergic asthma through transcription factors related to Th1 and Th2 |
| title_full_unstemmed | The mediatory role of Majie cataplasm on inflammation of allergic asthma through transcription factors related to Th1 and Th2 |
| title_short | The mediatory role of Majie cataplasm on inflammation of allergic asthma through transcription factors related to Th1 and Th2 |
| title_sort | mediatory role of majie cataplasm on inflammation of allergic asthma through transcription factors related to th1 and th2 |
| topic | Majie cataplasm Th2 inflammation of allergic asthma Th1/Th2 STAT6 GATA-3 STAT3 |
| url | http://link.springer.com/article/10.1186/s13020-020-00334-w |
| work_keys_str_mv | AT wentingji themediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT qianyizhang themediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT hanfenshi themediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT ruijuandong themediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT dongyuge themediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT xindu themediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT beidaren themediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT xueqianwang themediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT qingguowang themediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT wentingji mediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT qianyizhang mediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT hanfenshi mediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT ruijuandong mediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT dongyuge mediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT xindu mediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT beidaren mediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT xueqianwang mediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 AT qingguowang mediatoryroleofmajiecataplasmoninflammationofallergicasthmathroughtranscriptionfactorsrelatedtoth1andth2 |