THE STATE OF THE WATER IN BRAIN TISSUE IN PRESENCE OF TS-100 NANOPARTICLES
By the method of low-temperature 1Н NMR spectroscopy the structure of the hydrate layers of water associated with brain cells, the changes of these parameters during necrotic lesions (stroke) and in the presence of trifluoroacetic acid, which allows differentiating intracellular water clusters accor...
| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2015-12-01
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| Series: | Biotechnologia Acta |
| Subjects: | |
| Online Access: | http://biotechnology.kiev.ua/images/stories/6_2015/krupska_6_2015.pdf |
| _version_ | 1827848523532468224 |
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| author | T. V. S. V. O. V. O. T. V. V. |
| author_facet | T. V. S. V. O. V. O. T. V. V. |
| author_sort | T. V. |
| collection | DOAJ |
| description | By the method of low-temperature 1Н NMR spectroscopy the structure of the hydrate layers of water associated with brain cells, the changes of these parameters during necrotic lesions (stroke) and in the presence of trifluoroacetic acid, which allows differentiating intracellular water clusters according to their ability to dissolve the acid, were studied. Also the impact of silica TS-100 nanoparticles on the state of water in brain tissue, namely on the water binding parameters in the air and in the presence of a weakly polar solvent was considered.
The distributions by the radii and change of Gibbs free energy for clusters of strongly bound interfacial water were obtained. It was shown that the hydration properties of the native brain tissue differ from the hydration properties of necrotic damaged tissue by the structure of weakly bound water clusters. In intact tissue all the water is associated and is a part of clusters and domains, most of which have a radii R = 2 and 20 nm. The media with chloroform stabilizes water polyassociates with the radius up to R = 100 nm and trifluoroacetic acid stabilizes water polyassociates with radii R = 7–20 nm. It was found that the partial dehydration of the investigated tissue samples is accompanied by decreasing of weakly bound water amount and some increasing of strongly bound water that indicates a change of molecular interactions between the components of cells-nanoparticles composite system. The ischemic necrosis area presence leads to a decrease of water binding due to the average size water polyassociates increasing. This effect is observed both in air and in a weakly polar organic solvent medium (deuterochloroform). |
| first_indexed | 2024-03-12T09:48:21Z |
| format | Article |
| id | doaj.art-a2b65b0e98594903ae27f4260fb99c4c |
| institution | Directory Open Access Journal |
| issn | 2410-7751 2410-776X |
| language | English |
| last_indexed | 2024-03-12T09:48:21Z |
| publishDate | 2015-12-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | Biotechnologia Acta |
| spelling | doaj.art-a2b65b0e98594903ae27f4260fb99c4c2023-09-02T12:44:34ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryBiotechnologia Acta2410-77512410-776X2015-12-0186879910.15407/biotech8.06.087THE STATE OF THE WATER IN BRAIN TISSUE IN PRESENCE OF TS-100 NANOPARTICLEST. V. 0S. V. 1 O. V. 2O. T. 3 V. V. 4KrupskaPakrishenSerovVolikTurov By the method of low-temperature 1Н NMR spectroscopy the structure of the hydrate layers of water associated with brain cells, the changes of these parameters during necrotic lesions (stroke) and in the presence of trifluoroacetic acid, which allows differentiating intracellular water clusters according to their ability to dissolve the acid, were studied. Also the impact of silica TS-100 nanoparticles on the state of water in brain tissue, namely on the water binding parameters in the air and in the presence of a weakly polar solvent was considered. The distributions by the radii and change of Gibbs free energy for clusters of strongly bound interfacial water were obtained. It was shown that the hydration properties of the native brain tissue differ from the hydration properties of necrotic damaged tissue by the structure of weakly bound water clusters. In intact tissue all the water is associated and is a part of clusters and domains, most of which have a radii R = 2 and 20 nm. The media with chloroform stabilizes water polyassociates with the radius up to R = 100 nm and trifluoroacetic acid stabilizes water polyassociates with radii R = 7–20 nm. It was found that the partial dehydration of the investigated tissue samples is accompanied by decreasing of weakly bound water amount and some increasing of strongly bound water that indicates a change of molecular interactions between the components of cells-nanoparticles composite system. The ischemic necrosis area presence leads to a decrease of water binding due to the average size water polyassociates increasing. This effect is observed both in air and in a weakly polar organic solvent medium (deuterochloroform).http://biotechnology.kiev.ua/images/stories/6_2015/krupska_6_2015.pdfischemic strokethe strongly and weakly associated water1Н NMR spectroscopy. |
| spellingShingle | T. V. S. V. O. V. O. T. V. V. THE STATE OF THE WATER IN BRAIN TISSUE IN PRESENCE OF TS-100 NANOPARTICLES Biotechnologia Acta ischemic stroke the strongly and weakly associated water 1Н NMR spectroscopy. |
| title | THE STATE OF THE WATER IN BRAIN TISSUE IN PRESENCE OF TS-100 NANOPARTICLES |
| title_full | THE STATE OF THE WATER IN BRAIN TISSUE IN PRESENCE OF TS-100 NANOPARTICLES |
| title_fullStr | THE STATE OF THE WATER IN BRAIN TISSUE IN PRESENCE OF TS-100 NANOPARTICLES |
| title_full_unstemmed | THE STATE OF THE WATER IN BRAIN TISSUE IN PRESENCE OF TS-100 NANOPARTICLES |
| title_short | THE STATE OF THE WATER IN BRAIN TISSUE IN PRESENCE OF TS-100 NANOPARTICLES |
| title_sort | state of the water in brain tissue in presence of ts 100 nanoparticles |
| topic | ischemic stroke the strongly and weakly associated water 1Н NMR spectroscopy. |
| url | http://biotechnology.kiev.ua/images/stories/6_2015/krupska_6_2015.pdf |
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