Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of <it>Taylorella asinigenitalis</it>
<p>Abstract</p> <p>Background</p> <p>Sequence information on the 16S-23S rDNA internal spacer region (ISR) exhibits a large degree of sequence and length variation at both the genus and species levels. A primer pair for the amplification of 16S-23S rDNA ISR generated th...
| Main Authors: | , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
BMC
2009-03-01
|
| Series: | BMC Research Notes |
| Online Access: | http://www.biomedcentral.com/1756-0500/2/33 |
| _version_ | 1831541264680484864 |
|---|---|
| author | Millar B Cherie Moore John E Sekizuka Tsuyoshi Ono Shinji Tazumi Akihiro Matsuda Motoo |
| author_facet | Millar B Cherie Moore John E Sekizuka Tsuyoshi Ono Shinji Tazumi Akihiro Matsuda Motoo |
| author_sort | Millar B Cherie |
| collection | DOAJ |
| description | <p>Abstract</p> <p>Background</p> <p>Sequence information on the 16S-23S rDNA internal spacer region (ISR) exhibits a large degree of sequence and length variation at both the genus and species levels. A primer pair for the amplification of 16S-23S rDNA ISR generated three amplicons for each of isolates of <it>Taylorella asinigenitalis </it>(UCD-1<sup>T</sup>, UK-1 and UK-2).</p> <p>Findings</p> <p>Following TA cloning and sequencing, the three isolates of <it>T. asinigenitalis </it>were demonstrated to possess three ISR units of different lengths. Although the three corresponding ISRs (A, B and C) were identified to be identical to each other (UK-1 and UK-2 isolates), the ISRs shared approximately 95.3–98.9% nucleotide sequence similarities between the UCD-1<sup>T </sup>and UK-1/-2 isolates. A typical order of two intercistronic tRNA genes (5'-tRNA<sup>Ile</sup>-tRNA<sup>Ala</sup>-3') with the different nucleotide spacers [44 through 51 base pairs (bp)] in length was identified among the isolates. The consensus sequences of the antiterminators of <b>boxB </b>and <b>boxA </b>were also identified in all ISRs. Thus, three ISRs were identified for each isolate, and therefore, at least three distinctly different ribosomal RNA operons were suggested to occur in the genome of <it>T. asinigenitalis</it>. This was also confirmed by Southern hybridization procedure.</p> <p>Conclusion</p> <p>The present study represents a dendrogram constructed based on the nucleotide sequence data of 16S-23S rDNA ISR for <it>T. asinigenitalis</it>, which may aid in the phylogenetic positioning of <it>T. asinigenitalis </it>within the genus <it>Taylorella</it>, and in the molecular discrimination of <it>T. asinigenitalis</it>.</p> |
| first_indexed | 2024-12-17T00:13:44Z |
| format | Article |
| id | doaj.art-a2b73967be5c4dff805d591f467ea27d |
| institution | Directory Open Access Journal |
| issn | 1756-0500 |
| language | English |
| last_indexed | 2024-12-17T00:13:44Z |
| publishDate | 2009-03-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Research Notes |
| spelling | doaj.art-a2b73967be5c4dff805d591f467ea27d2022-12-21T22:10:45ZengBMCBMC Research Notes1756-05002009-03-01213310.1186/1756-0500-2-33Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of <it>Taylorella asinigenitalis</it>Millar B CherieMoore John ESekizuka TsuyoshiOno ShinjiTazumi AkihiroMatsuda Motoo<p>Abstract</p> <p>Background</p> <p>Sequence information on the 16S-23S rDNA internal spacer region (ISR) exhibits a large degree of sequence and length variation at both the genus and species levels. A primer pair for the amplification of 16S-23S rDNA ISR generated three amplicons for each of isolates of <it>Taylorella asinigenitalis </it>(UCD-1<sup>T</sup>, UK-1 and UK-2).</p> <p>Findings</p> <p>Following TA cloning and sequencing, the three isolates of <it>T. asinigenitalis </it>were demonstrated to possess three ISR units of different lengths. Although the three corresponding ISRs (A, B and C) were identified to be identical to each other (UK-1 and UK-2 isolates), the ISRs shared approximately 95.3–98.9% nucleotide sequence similarities between the UCD-1<sup>T </sup>and UK-1/-2 isolates. A typical order of two intercistronic tRNA genes (5'-tRNA<sup>Ile</sup>-tRNA<sup>Ala</sup>-3') with the different nucleotide spacers [44 through 51 base pairs (bp)] in length was identified among the isolates. The consensus sequences of the antiterminators of <b>boxB </b>and <b>boxA </b>were also identified in all ISRs. Thus, three ISRs were identified for each isolate, and therefore, at least three distinctly different ribosomal RNA operons were suggested to occur in the genome of <it>T. asinigenitalis</it>. This was also confirmed by Southern hybridization procedure.</p> <p>Conclusion</p> <p>The present study represents a dendrogram constructed based on the nucleotide sequence data of 16S-23S rDNA ISR for <it>T. asinigenitalis</it>, which may aid in the phylogenetic positioning of <it>T. asinigenitalis </it>within the genus <it>Taylorella</it>, and in the molecular discrimination of <it>T. asinigenitalis</it>.</p>http://www.biomedcentral.com/1756-0500/2/33 |
| spellingShingle | Millar B Cherie Moore John E Sekizuka Tsuyoshi Ono Shinji Tazumi Akihiro Matsuda Motoo Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of <it>Taylorella asinigenitalis</it> BMC Research Notes |
| title | Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of <it>Taylorella asinigenitalis</it> |
| title_full | Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of <it>Taylorella asinigenitalis</it> |
| title_fullStr | Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of <it>Taylorella asinigenitalis</it> |
| title_full_unstemmed | Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of <it>Taylorella asinigenitalis</it> |
| title_short | Molecular characterization of the sequences of the 16S-23S rDNA internal spacer region (ISR) from isolates of <it>Taylorella asinigenitalis</it> |
| title_sort | molecular characterization of the sequences of the 16s 23s rdna internal spacer region isr from isolates of it taylorella asinigenitalis it |
| url | http://www.biomedcentral.com/1756-0500/2/33 |
| work_keys_str_mv | AT millarbcherie molecularcharacterizationofthesequencesofthe16s23srdnainternalspacerregionisrfromisolatesofittaylorellaasinigenitalisit AT moorejohne molecularcharacterizationofthesequencesofthe16s23srdnainternalspacerregionisrfromisolatesofittaylorellaasinigenitalisit AT sekizukatsuyoshi molecularcharacterizationofthesequencesofthe16s23srdnainternalspacerregionisrfromisolatesofittaylorellaasinigenitalisit AT onoshinji molecularcharacterizationofthesequencesofthe16s23srdnainternalspacerregionisrfromisolatesofittaylorellaasinigenitalisit AT tazumiakihiro molecularcharacterizationofthesequencesofthe16s23srdnainternalspacerregionisrfromisolatesofittaylorellaasinigenitalisit AT matsudamotoo molecularcharacterizationofthesequencesofthe16s23srdnainternalspacerregionisrfromisolatesofittaylorellaasinigenitalisit |