Restoration of IL-11 and IL-15 cytokine production post calcium modulators and ROS treatment can assist viral clearance both in vitro and in vivo
Objective(s): Hepatitis B virus (HBV) infection alters the cytokines production to establish persistent infection. A reversion of cytokines back to their normal state can be a promising therapeutic approach to establish an optimal host immune response. Materials and Methods: We investigated the alte...
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| Format: | Article |
| Language: | English |
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Mashhad University of Medical Sciences
2023-02-01
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| Series: | Iranian Journal of Basic Medical Sciences |
| Subjects: | |
| Online Access: | https://ijbms.mums.ac.ir/article_21418_8e1118541fcb5aa44699c97d8df6c157.pdf |
| _version_ | 1797977637445959680 |
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| author | Kehkshan Jabeen Aneela Javed Asim Waris Shaheen Shahzad |
| author_facet | Kehkshan Jabeen Aneela Javed Asim Waris Shaheen Shahzad |
| author_sort | Kehkshan Jabeen |
| collection | DOAJ |
| description | Objective(s): Hepatitis B virus (HBV) infection alters the cytokines production to establish persistent infection. A reversion of cytokines back to their normal state can be a promising therapeutic approach to establish an optimal host immune response. Materials and Methods: We investigated the alteration in expression of IL-15 and IL-11 after HBV infection in vitro and in vivo in PBMCs of 63 individuals divided into various HBV-infected patient groups. The mRNA expression was evaluated post-anti-oxidant and calcium modulators treatment by Real-time qPCR. Results: In vitro mRNA expression of both cytokines, post-infection was down-regulated considerably. Interestingly, in line with in vitro results, both cytokines’ in vivo expression was intensively down-regulated in chronic HBV-infected individuals rather than healthy controls. Both cytokines’ expression was up-regulated in cases of recovery compared with the inactive carriers and chronic HBV-infected individuals. IL-15 mRNA expression was significantly up-regulated in both cell lines post EGTA and Ru360 treatment while a significant increase was observed in the HepAD38 cell line with NAC and BAPTA treatment. IL-11 mRNA expression was significantly up-regulated in the HepG2 cell line after all modulator treatments, whereas in the HepAd38 cell line it was observed after BAPTA treatment. Our results thus indicate that viral infection tends to down-regulate the expression of cytokines and an in vivo up-regulation is an indication of recovery. Conclusion: Treatment of anti-oxidants and calcium modulators has resulted in the successful restoration of these cytokines thus pointing towards the use of calcium modulators to boost natural antiviral cytokine production. |
| first_indexed | 2024-04-11T05:11:00Z |
| format | Article |
| id | doaj.art-a2b79f367ff14979b481c88142ef9b25 |
| institution | Directory Open Access Journal |
| issn | 2008-3866 2008-3874 |
| language | English |
| last_indexed | 2024-04-11T05:11:00Z |
| publishDate | 2023-02-01 |
| publisher | Mashhad University of Medical Sciences |
| record_format | Article |
| series | Iranian Journal of Basic Medical Sciences |
| spelling | doaj.art-a2b79f367ff14979b481c88142ef9b252022-12-25T11:14:35ZengMashhad University of Medical SciencesIranian Journal of Basic Medical Sciences2008-38662008-38742023-02-0126217618210.22038/ijbms.2022.65983.1453621418Restoration of IL-11 and IL-15 cytokine production post calcium modulators and ROS treatment can assist viral clearance both in vitro and in vivoKehkshan Jabeen0Aneela Javed1Asim Waris2Shaheen Shahzad3Genomics Research Lab, Department of Biological Sciences, International Islamic University Islamabad, Islamabad, PakistanHealthcare Biotechnology, Atta-ur Rahman School of Applied Biosciences (ASAB), National University of Sciences and Technology (NUST), H-12 Campus, Islamabad, PakistanSchool of Mechanical & Manufacturing Engineering (SMME), National University of Sciences and Technology (NUST), H-12 Campus, Islamabad, PakistanGenomics Research Lab, Department of Biological Sciences, International Islamic University Islamabad, Islamabad, PakistanObjective(s): Hepatitis B virus (HBV) infection alters the cytokines production to establish persistent infection. A reversion of cytokines back to their normal state can be a promising therapeutic approach to establish an optimal host immune response. Materials and Methods: We investigated the alteration in expression of IL-15 and IL-11 after HBV infection in vitro and in vivo in PBMCs of 63 individuals divided into various HBV-infected patient groups. The mRNA expression was evaluated post-anti-oxidant and calcium modulators treatment by Real-time qPCR. Results: In vitro mRNA expression of both cytokines, post-infection was down-regulated considerably. Interestingly, in line with in vitro results, both cytokines’ in vivo expression was intensively down-regulated in chronic HBV-infected individuals rather than healthy controls. Both cytokines’ expression was up-regulated in cases of recovery compared with the inactive carriers and chronic HBV-infected individuals. IL-15 mRNA expression was significantly up-regulated in both cell lines post EGTA and Ru360 treatment while a significant increase was observed in the HepAD38 cell line with NAC and BAPTA treatment. IL-11 mRNA expression was significantly up-regulated in the HepG2 cell line after all modulator treatments, whereas in the HepAd38 cell line it was observed after BAPTA treatment. Our results thus indicate that viral infection tends to down-regulate the expression of cytokines and an in vivo up-regulation is an indication of recovery. Conclusion: Treatment of anti-oxidants and calcium modulators has resulted in the successful restoration of these cytokines thus pointing towards the use of calcium modulators to boost natural antiviral cytokine production.https://ijbms.mums.ac.ir/article_21418_8e1118541fcb5aa44699c97d8df6c157.pdfhepatitis b virusinterleukin-11interleukin-15hepatocellular carcinomareactive oxygen species |
| spellingShingle | Kehkshan Jabeen Aneela Javed Asim Waris Shaheen Shahzad Restoration of IL-11 and IL-15 cytokine production post calcium modulators and ROS treatment can assist viral clearance both in vitro and in vivo Iranian Journal of Basic Medical Sciences hepatitis b virus interleukin-11 interleukin-15 hepatocellular carcinoma reactive oxygen species |
| title | Restoration of IL-11 and IL-15 cytokine production post calcium modulators and ROS treatment can assist viral clearance both in vitro and in vivo |
| title_full | Restoration of IL-11 and IL-15 cytokine production post calcium modulators and ROS treatment can assist viral clearance both in vitro and in vivo |
| title_fullStr | Restoration of IL-11 and IL-15 cytokine production post calcium modulators and ROS treatment can assist viral clearance both in vitro and in vivo |
| title_full_unstemmed | Restoration of IL-11 and IL-15 cytokine production post calcium modulators and ROS treatment can assist viral clearance both in vitro and in vivo |
| title_short | Restoration of IL-11 and IL-15 cytokine production post calcium modulators and ROS treatment can assist viral clearance both in vitro and in vivo |
| title_sort | restoration of il 11 and il 15 cytokine production post calcium modulators and ros treatment can assist viral clearance both in vitro and in vivo |
| topic | hepatitis b virus interleukin-11 interleukin-15 hepatocellular carcinoma reactive oxygen species |
| url | https://ijbms.mums.ac.ir/article_21418_8e1118541fcb5aa44699c97d8df6c157.pdf |
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