Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane
The influences of various factors on blood lead to the formation of extra reactive oxygen species (ROS), resulting in the disruption of morphology and functions of red blood cells (RBCs). This study considers the mechanisms of the mechanochemical synergism of <inline-formula><math xmlns=&qu...
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| Format: | Article |
| Language: | English |
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MDPI AG
2023-03-01
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| Series: | International Journal of Molecular Sciences |
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| Online Access: | https://www.mdpi.com/1422-0067/24/6/5952 |
| _version_ | 1797611185205411840 |
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| author | Elena Kozlova Viktoria Sergunova Ekaterina Sherstyukova Andrey Grechko Snezhanna Lyapunova Vladimir Inozemtsev Aleksandr Kozlov Olga Gudkova Aleksandr Chernysh |
| author_facet | Elena Kozlova Viktoria Sergunova Ekaterina Sherstyukova Andrey Grechko Snezhanna Lyapunova Vladimir Inozemtsev Aleksandr Kozlov Olga Gudkova Aleksandr Chernysh |
| author_sort | Elena Kozlova |
| collection | DOAJ |
| description | The influences of various factors on blood lead to the formation of extra reactive oxygen species (ROS), resulting in the disruption of morphology and functions of red blood cells (RBCs). This study considers the mechanisms of the mechanochemical synergism of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>O</mi><msup><mi>H</mi><mo>•</mo></msup></mrow></semantics></math></inline-formula> free radicals, which are most active in the initiation of lipid peroxidation (LPO) in RBC membranes, and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>H</mi><mn>2</mn></msub><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> molecules, the largest typical diffusion path. Using kinetic models of differential equations describing <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>C</mi><mrow><msub><mi>H</mi><mn>2</mn></msub><msub><mi>O</mi><mn>2</mn></msub></mrow></msub><mfenced><mi>t</mi></mfenced><mo> </mo></mrow></semantics></math></inline-formula> and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>C</mi><mrow><mi>O</mi><msup><mi>H</mi><mo>•</mo></msup></mrow></msub><mfenced><mi>t</mi></mfenced></mrow></semantics></math></inline-formula>, we discuss two levels of mechanochemical synergism that occur simultaneously: (1) synergism that ensures the delivery of highly active free radicals <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>O</mi><msup><mi>H</mi><mo>•</mo></msup></mrow></semantics></math></inline-formula> to RBC membranes and (2) a positive feedback system between <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>H</mi><mn>2</mn></msub><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>O</mi><msup><mi>H</mi><mo>•</mo></msup></mrow></semantics></math></inline-formula>, resulting in the partial restoration of spent molecules. As a result of these ROS synergisms, the efficiency of LPO in RBC membranes sharply increases. In blood, the appearance of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>O</mi><msup><mi>H</mi><mo>•</mo></msup></mrow></semantics></math></inline-formula> free radicals is due to the interaction of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>H</mi><mn>2</mn></msub><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> molecules with free iron ions (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>F</mi><msup><mi>e</mi><mrow><mn>2</mn><mo>+</mo></mrow></msup></mrow></semantics></math></inline-formula>) which arise as a result of heme degradation. We experimentally established the quantitative dependences of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>C</mi><mrow><mi>O</mi><msup><mi>H</mi><mo>•</mo></msup><mo> </mo></mrow></msub><mfenced><mrow><msub><mi>C</mi><mrow><msub><mi>H</mi><mn>2</mn></msub><msub><mi>O</mi><mn>2</mn></msub></mrow></msub></mrow></mfenced></mrow></semantics></math></inline-formula> using the methods of spectrophotometry and nonlinear curve fitting. This study extends the analysis of the influence of ROS mechanisms in RBC suspensions. |
| first_indexed | 2024-03-11T06:24:16Z |
| format | Article |
| id | doaj.art-a2b91e9440304452a855295f3aaf63fe |
| institution | Directory Open Access Journal |
| issn | 1661-6596 1422-0067 |
| language | English |
| last_indexed | 2024-03-11T06:24:16Z |
| publishDate | 2023-03-01 |
| publisher | MDPI AG |
| record_format | Article |
| series | International Journal of Molecular Sciences |
| spelling | doaj.art-a2b91e9440304452a855295f3aaf63fe2023-11-17T11:41:25ZengMDPI AGInternational Journal of Molecular Sciences1661-65961422-00672023-03-01246595210.3390/ijms24065952Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC MembraneElena Kozlova0Viktoria Sergunova1Ekaterina Sherstyukova2Andrey Grechko3Snezhanna Lyapunova4Vladimir Inozemtsev5Aleksandr Kozlov6Olga Gudkova7Aleksandr Chernysh8Laboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, RussiaLaboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, RussiaLaboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, RussiaAdministration, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, 107031 Moscow, RussiaLaboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, RussiaLaboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, RussiaDepartment of Medical and Biological Physics, Sechenov First Moscow State Medical University (Sechenov University), 119991 Moscow, RussiaLaboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, RussiaLaboratory of Biophysics of Cell Membranes under Critical State, Federal Research and Clinical Center of Intensive Care Medicine and Rehabilitology, V.A. Negovsky Research Institute of General Reanimatology, 107031 Moscow, RussiaThe influences of various factors on blood lead to the formation of extra reactive oxygen species (ROS), resulting in the disruption of morphology and functions of red blood cells (RBCs). This study considers the mechanisms of the mechanochemical synergism of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>O</mi><msup><mi>H</mi><mo>•</mo></msup></mrow></semantics></math></inline-formula> free radicals, which are most active in the initiation of lipid peroxidation (LPO) in RBC membranes, and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>H</mi><mn>2</mn></msub><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> molecules, the largest typical diffusion path. Using kinetic models of differential equations describing <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>C</mi><mrow><msub><mi>H</mi><mn>2</mn></msub><msub><mi>O</mi><mn>2</mn></msub></mrow></msub><mfenced><mi>t</mi></mfenced><mo> </mo></mrow></semantics></math></inline-formula> and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>C</mi><mrow><mi>O</mi><msup><mi>H</mi><mo>•</mo></msup></mrow></msub><mfenced><mi>t</mi></mfenced></mrow></semantics></math></inline-formula>, we discuss two levels of mechanochemical synergism that occur simultaneously: (1) synergism that ensures the delivery of highly active free radicals <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>O</mi><msup><mi>H</mi><mo>•</mo></msup></mrow></semantics></math></inline-formula> to RBC membranes and (2) a positive feedback system between <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>H</mi><mn>2</mn></msub><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> and <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>O</mi><msup><mi>H</mi><mo>•</mo></msup></mrow></semantics></math></inline-formula>, resulting in the partial restoration of spent molecules. As a result of these ROS synergisms, the efficiency of LPO in RBC membranes sharply increases. In blood, the appearance of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>O</mi><msup><mi>H</mi><mo>•</mo></msup></mrow></semantics></math></inline-formula> free radicals is due to the interaction of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>H</mi><mn>2</mn></msub><msub><mi>O</mi><mn>2</mn></msub></mrow></semantics></math></inline-formula> molecules with free iron ions (<inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><mi>F</mi><msup><mi>e</mi><mrow><mn>2</mn><mo>+</mo></mrow></msup></mrow></semantics></math></inline-formula>) which arise as a result of heme degradation. We experimentally established the quantitative dependences of <inline-formula><math xmlns="http://www.w3.org/1998/Math/MathML" display="inline"><semantics><mrow><msub><mi>C</mi><mrow><mi>O</mi><msup><mi>H</mi><mo>•</mo></msup><mo> </mo></mrow></msub><mfenced><mrow><msub><mi>C</mi><mrow><msub><mi>H</mi><mn>2</mn></msub><msub><mi>O</mi><mn>2</mn></msub></mrow></msub></mrow></mfenced></mrow></semantics></math></inline-formula> using the methods of spectrophotometry and nonlinear curve fitting. This study extends the analysis of the influence of ROS mechanisms in RBC suspensions.https://www.mdpi.com/1422-0067/24/6/5952RBC membranesROSmechanochemical synergismkinetic modelhydroxyl radicalhydrogen peroxide |
| spellingShingle | Elena Kozlova Viktoria Sergunova Ekaterina Sherstyukova Andrey Grechko Snezhanna Lyapunova Vladimir Inozemtsev Aleksandr Kozlov Olga Gudkova Aleksandr Chernysh Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane International Journal of Molecular Sciences RBC membranes ROS mechanochemical synergism kinetic model hydroxyl radical hydrogen peroxide |
| title | Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane |
| title_full | Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane |
| title_fullStr | Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane |
| title_full_unstemmed | Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane |
| title_short | Mechanochemical Synergism of Reactive Oxygen Species Influences on RBC Membrane |
| title_sort | mechanochemical synergism of reactive oxygen species influences on rbc membrane |
| topic | RBC membranes ROS mechanochemical synergism kinetic model hydroxyl radical hydrogen peroxide |
| url | https://www.mdpi.com/1422-0067/24/6/5952 |
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