Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats
Abstract Background Neurological disorders suggest that the excitotoxicity involves a drastic increase in intracellular Ca2+ concentrations and the formation of reactive oxygen species. The presence of these free radicals may also affect the dopaminergic system. The aim of this work was to determine...
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BMC
2018-11-01
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| Series: | BMC Neuroscience |
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| Online Access: | http://link.springer.com/article/10.1186/s12868-018-0474-4 |
| _version_ | 1818336052552663040 |
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| author | Armando Valenzuela Peraza David Calderón Guzmán Norma Osnaya Brizuela Maribel Ortiz Herrera Hugo Juárez Olguín Miroslava Lindoro Silva Belén Juárez Tapia Gerardo Barragán Mejía |
| author_facet | Armando Valenzuela Peraza David Calderón Guzmán Norma Osnaya Brizuela Maribel Ortiz Herrera Hugo Juárez Olguín Miroslava Lindoro Silva Belén Juárez Tapia Gerardo Barragán Mejía |
| author_sort | Armando Valenzuela Peraza |
| collection | DOAJ |
| description | Abstract Background Neurological disorders suggest that the excitotoxicity involves a drastic increase in intracellular Ca2+ concentrations and the formation of reactive oxygen species. The presence of these free radicals may also affect the dopaminergic system. The aim of this work was to determine if riboflavin (B2) and pyridoxine (B6) provide protection to the brain against free radicals generated by 3-nitropropionic acid (3-NPA) by measuring the levels of dopamine (DA) and selected oxidative stress markers. Methods Male Fisher rats were grouped (n = 6) and treated as follows: group 1, control (NaCl 0.9%); group 2, 3-NPA (20 mg/kg); group 3, B2 (10 mg/kg); group 4, B2 (10 mg/kg) + 3-NPA (20 mg/kg); group 5, B6 (10 mg/kg) and group 6, B6 + 3-NPA. All treatments were administered every 24 h for 5 days by intraperitoneal route. After sacrifice, the brain was obtained to measure DA, GSH, and lipid peroxidation, Ca2+, Mg2+, ATPase and H2O2. Main findings Levels of dopamine increased in cortex, striatum and cerebellum/medulla oblongata of animals that received 3-NPA alone. The lipid peroxidation increased in cortex, striatum, and cerebellum/medulla oblongata, of animals treated with B2 vitamin alone. ATPase dependent on Ca+2, Mg+2 and H2O2 increased in all regions of animals that received 3-NPA alone. Conclusion The results confirm the capacity of 3-NPA to generate oxidative stress. Besides, the study suggests that B2 or B6 vitamins restored the levels of DA and reduced oxidative stress in brain of rats. We believe that these results would help in the study of neurodegenerative diseases. |
| first_indexed | 2024-12-13T14:33:11Z |
| format | Article |
| id | doaj.art-a2bb42752eb344b7b5e6fe5b67d9470c |
| institution | Directory Open Access Journal |
| issn | 1471-2202 |
| language | English |
| last_indexed | 2024-12-13T14:33:11Z |
| publishDate | 2018-11-01 |
| publisher | BMC |
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| series | BMC Neuroscience |
| spelling | doaj.art-a2bb42752eb344b7b5e6fe5b67d9470c2022-12-21T23:41:47ZengBMCBMC Neuroscience1471-22022018-11-011911810.1186/s12868-018-0474-4Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of ratsArmando Valenzuela Peraza0David Calderón Guzmán1Norma Osnaya Brizuela2Maribel Ortiz Herrera3Hugo Juárez Olguín4Miroslava Lindoro Silva5Belén Juárez Tapia6Gerardo Barragán Mejía7Laboratorio de Neurociencias, Instituto Nacional de Pediatría (INP)Laboratorio de Neurociencias, Instituto Nacional de Pediatría (INP)Laboratorio de Neurociencias, Instituto Nacional de Pediatría (INP)Laboratorio de Bacteriología Experimental, INPLaboratorio de Farmacología, Instituto Nacional de Pediatría (INP), y Facultad de Medicina, Universidad Nacional Autónoma de MéxicoLaboratorio de Farmacología, Instituto Nacional de Pediatría (INP), y Facultad de Medicina, Universidad Nacional Autónoma de MéxicoLaboratorio de Farmacología, Instituto Nacional de Pediatría (INP), y Facultad de Medicina, Universidad Nacional Autónoma de MéxicoLaboratorio de Neurociencias, Instituto Nacional de Pediatría (INP)Abstract Background Neurological disorders suggest that the excitotoxicity involves a drastic increase in intracellular Ca2+ concentrations and the formation of reactive oxygen species. The presence of these free radicals may also affect the dopaminergic system. The aim of this work was to determine if riboflavin (B2) and pyridoxine (B6) provide protection to the brain against free radicals generated by 3-nitropropionic acid (3-NPA) by measuring the levels of dopamine (DA) and selected oxidative stress markers. Methods Male Fisher rats were grouped (n = 6) and treated as follows: group 1, control (NaCl 0.9%); group 2, 3-NPA (20 mg/kg); group 3, B2 (10 mg/kg); group 4, B2 (10 mg/kg) + 3-NPA (20 mg/kg); group 5, B6 (10 mg/kg) and group 6, B6 + 3-NPA. All treatments were administered every 24 h for 5 days by intraperitoneal route. After sacrifice, the brain was obtained to measure DA, GSH, and lipid peroxidation, Ca2+, Mg2+, ATPase and H2O2. Main findings Levels of dopamine increased in cortex, striatum and cerebellum/medulla oblongata of animals that received 3-NPA alone. The lipid peroxidation increased in cortex, striatum, and cerebellum/medulla oblongata, of animals treated with B2 vitamin alone. ATPase dependent on Ca+2, Mg+2 and H2O2 increased in all regions of animals that received 3-NPA alone. Conclusion The results confirm the capacity of 3-NPA to generate oxidative stress. Besides, the study suggests that B2 or B6 vitamins restored the levels of DA and reduced oxidative stress in brain of rats. We believe that these results would help in the study of neurodegenerative diseases.http://link.springer.com/article/10.1186/s12868-018-0474-4BrainHuntington animal modelOxidative stressRiboflavinPyridoxine |
| spellingShingle | Armando Valenzuela Peraza David Calderón Guzmán Norma Osnaya Brizuela Maribel Ortiz Herrera Hugo Juárez Olguín Miroslava Lindoro Silva Belén Juárez Tapia Gerardo Barragán Mejía Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats BMC Neuroscience Brain Huntington animal model Oxidative stress Riboflavin Pyridoxine |
| title | Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
| title_full | Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
| title_fullStr | Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
| title_full_unstemmed | Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
| title_short | Riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
| title_sort | riboflavin and pyridoxine restore dopamine levels and reduce oxidative stress in brain of rats |
| topic | Brain Huntington animal model Oxidative stress Riboflavin Pyridoxine |
| url | http://link.springer.com/article/10.1186/s12868-018-0474-4 |
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