A molecular marker set combining a retrotransposon insertion and SSR polymorphisms is useful for assessing diversity in Vitis
Molecular markers, based on DNA polymorphisms, are useful tools for identifying individuals, establishing phylogenetic relationships, managing collections of genetic material or assisting breeding. In the present study, we developed a marker set to differentiate Vitis species, grapevine varieties or...
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| Format: | Article |
| Language: | English |
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International Viticulture and Enology Society
2021-06-01
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| Series: | OENO One |
| Subjects: | |
| Online Access: | https://oeno-one.eu/article/view/4473 |
| _version_ | 1819292473268109312 |
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| author | Frederique Pelsy Lucie Bevilacqua Sophie Blanc Didier Merdinoglu |
| author_facet | Frederique Pelsy Lucie Bevilacqua Sophie Blanc Didier Merdinoglu |
| author_sort | Frederique Pelsy |
| collection | DOAJ |
| description | Molecular markers, based on DNA polymorphisms, are useful tools for identifying individuals, establishing phylogenetic relationships, managing collections of genetic material or assisting breeding. In the present study, we developed a marker set to differentiate Vitis species, grapevine varieties or clones belonging to the same variety. This novel marker set combines, in four PCR amplifications, the presence/absence of a remarkable retrotransposon, Tvv1-Δ3460, inserted at its single locus and the SSR polymorphism present within its two LTRs. By studying a collection of Vitaceaeaccessions, we showed the prevalence of two allelic forms of Tvv1-Δ3460 - one of which was partially truncated - in Vitis species. Out of the twenty-five studied Vitis species, the insertion of a Tvv1-Δ3460 element was detected in twenty, including Vitis vinifera. The homozygous vs heterozygous state of the element insertion was determined by amplifying the empty site. Additionally, each Tvv1-Δ3460 LTRs included a microsatellite sequence useful for designing markers based on LTR length. The LTR-SSR markers distinguished most of the fifty-two cultivars and revealed polymorphism within five of the seven varieties studied. |
| first_indexed | 2024-12-24T03:55:05Z |
| format | Article |
| id | doaj.art-a2bca61251bb493c9918e3b1ca1deda9 |
| institution | Directory Open Access Journal |
| issn | 2494-1271 |
| language | English |
| last_indexed | 2024-12-24T03:55:05Z |
| publishDate | 2021-06-01 |
| publisher | International Viticulture and Enology Society |
| record_format | Article |
| series | OENO One |
| spelling | doaj.art-a2bca61251bb493c9918e3b1ca1deda92022-12-21T17:16:28ZengInternational Viticulture and Enology SocietyOENO One2494-12712021-06-0155210.20870/oeno-one.2021.55.2.4473A molecular marker set combining a retrotransposon insertion and SSR polymorphisms is useful for assessing diversity in VitisFrederique Pelsy0Lucie Bevilacqua1Sophie Blanc2Didier Merdinoglu3Université de Strasbourg, INRAE, SVQV UMR-A 1131, F-68000 ColmarUniversité de Strasbourg, INRAE, SVQV UMR-A 1131, F-68000 ColmarUniversité de Strasbourg, INRAE, SVQV UMR-A 1131, F-68000 ColmarUniversité de Strasbourg, INRAE, SVQV UMR-A 1131, F-68000 ColmarMolecular markers, based on DNA polymorphisms, are useful tools for identifying individuals, establishing phylogenetic relationships, managing collections of genetic material or assisting breeding. In the present study, we developed a marker set to differentiate Vitis species, grapevine varieties or clones belonging to the same variety. This novel marker set combines, in four PCR amplifications, the presence/absence of a remarkable retrotransposon, Tvv1-Δ3460, inserted at its single locus and the SSR polymorphism present within its two LTRs. By studying a collection of Vitaceaeaccessions, we showed the prevalence of two allelic forms of Tvv1-Δ3460 - one of which was partially truncated - in Vitis species. Out of the twenty-five studied Vitis species, the insertion of a Tvv1-Δ3460 element was detected in twenty, including Vitis vinifera. The homozygous vs heterozygous state of the element insertion was determined by amplifying the empty site. Additionally, each Tvv1-Δ3460 LTRs included a microsatellite sequence useful for designing markers based on LTR length. The LTR-SSR markers distinguished most of the fifty-two cultivars and revealed polymorphism within five of the seven varieties studied.https://oeno-one.eu/article/view/4473molecular markersgrapevineretrotransposonSRR |
| spellingShingle | Frederique Pelsy Lucie Bevilacqua Sophie Blanc Didier Merdinoglu A molecular marker set combining a retrotransposon insertion and SSR polymorphisms is useful for assessing diversity in Vitis OENO One molecular markers grapevine retrotransposon SRR |
| title | A molecular marker set combining a retrotransposon insertion and SSR polymorphisms is useful for assessing diversity in Vitis |
| title_full | A molecular marker set combining a retrotransposon insertion and SSR polymorphisms is useful for assessing diversity in Vitis |
| title_fullStr | A molecular marker set combining a retrotransposon insertion and SSR polymorphisms is useful for assessing diversity in Vitis |
| title_full_unstemmed | A molecular marker set combining a retrotransposon insertion and SSR polymorphisms is useful for assessing diversity in Vitis |
| title_short | A molecular marker set combining a retrotransposon insertion and SSR polymorphisms is useful for assessing diversity in Vitis |
| title_sort | molecular marker set combining a retrotransposon insertion and ssr polymorphisms is useful for assessing diversity in vitis |
| topic | molecular markers grapevine retrotransposon SRR |
| url | https://oeno-one.eu/article/view/4473 |
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