PERKEMBANGAN ENZIM PENCERNAAN LARVA IKAN PATIN, Pangasius hypophthalmus sp.
Development of digestive enzymes; protease, lipase and amylase were observed in patin catfish, Pangasius hypophthalmus, larvae. The 1 day old larvae (day after hatching), with 3,37-3,97 mm length and 0,62-0,79 mg weight, were reared in aquarium 60x50x40 cm with stocking density of 20 fish/l. Larv...
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| Format: | Article |
| Language: | English |
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Asosiasi Sains Akuakultur Indonesia
2003-05-01
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| Series: | Jurnal Akuakultur Indonesia |
| Online Access: | https://journal.ipb.ac.id/index.php/jai/article/view/3863 |
| _version_ | 1826856401015668736 |
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| author | Irzal Effendi . Widanarni D. Augustine |
| author_facet | Irzal Effendi . Widanarni D. Augustine |
| author_sort | Irzal Effendi |
| collection | DOAJ |
| description | Development of digestive enzymes; protease, lipase and amylase were observed in patin catfish, Pangasius hypophthalmus, larvae. The 1 day old larvae (day after hatching), with 3,37-3,97 mm length and 0,62-0,79 mg weight, were reared in aquarium 60x50x40 cm with stocking density of 20 fish/l. Larvae were fed Artemia dan tubificid worms 2-8 dan 7-15 days after hatching (dAH), respectively (schedule I); 2-6 and 5-15 dAH (schedule II); and 2-4 and 5-15 dAH (schedule III). Chlorella was ready to eat by larvae at the entirely rearing. For enzyme assay, larvae were sampled from each aquarium at stages of 1, 2, 3, 5, 7, 10 and 15 dAH. Protease and lipase activity were detected in digestive tract of 1 dAH larvae. Digestive enzymes development have a similar pattern in larvae for all feeding schedules. Protease activity decreased with the increasing of age until 3 dAH, then increased until the larvae reached 7 dAH, and sharply decreased until 10 dAH and then slowly decreased thereafter. Lipase activity tended to increase slowly with age up to 3 dAH, and increased sharply until 5 dAH, and then decreased sharply until 7 dAH before decreased again up to the end of rearing. Amylase activity in larvae increased slowly with the increasing of age up to 5 dAH, then increased sharply until 7 dAH, and decreased thereafter. In dimly lighted larvae, amylase activity decreased before increased up to 12 d AH, then decreased thereafter. The amount of food organisms in larval gut, body weight and length, and survival rate of larvae were also measured and discussed.
Key Words: Digestive enzymes, development, larvae, patin catfish, Pangasius hypophthalmus
ABSTRAKPenelitian ini bertujuan untuk mengetahui perkembangan enzim protease, lipase dan amilase saluran pencernaan larva ikan patin akibat perubahan skedul pemberian pakan. Larva ikan patin (panjang 3,77–3,97 mm dan bobot 0,62-0,79 mg) berumur 1 hari dipelihara di akuarium 60x50x40 cm dengan kepadatan 20 ekor/l. Larva diberi pakan dengan tiga skedul pemberian; skedul I, Artemia dan cacing diberikan ketika larva ikan patin berumur masing-masing 2-8 dan 7-15 hari; skedul II, Artemia 2-6 hari dan cacing 5-15 hari; skedul III, Artemia 2-4 hari dan cacing 5-15 hari. Chlorella diberikan sepanjang pemeliharaan larva. Contoh larva umur 1, 2, 3, 5, 7, 10 dan 15 hari diambil sebanyak 0,5 g setelah dipuasakan selama 4 jam. Asai protease, lipase dan amilase terhadap homogenate larva dilakukan dengan menggunakan masing-masing substrat kasein 1%, minyak kelapa sawit dan pati 1%. Anatomi, isi saluran pencernaan, pertumbuhan bobot dan kelangsungan hidup larva juga diamati. Tidak terdapat perbedaan pertumbuhan larva yang diberi pakan dengan skedul berbeda, namun larva yang diberi Artemia lebih lama (skedul I) memiliki kelangsungan hidup lebih baik. Larva ikan patin umur 1 hari setelah menetas ternyata sudah mengandung enzim protease dan lipase di saluran pencernaannya. Perkembangan enzim pencernaan memiliki pola yang hampir sama pada setiap skedul pemberian pakan. Aktivitas protease menurun pada larva umur 3 hari, selanjutnya meningkat tajam hingga larva umur 7 hari, kemudian menurun tajam hingga larva umur 10 hari dan akhirnya menurun landai. Aktivitas lipase meningkat lambat hingga larva umur 3 hari, kemudian meningkat tajam hingga larva umur 5 hari, selanjutnya menurun tajam hingga larva umur 7 hari dan akhirnya menurun landai. Aktivitas amilase semakin meningkat lambat dengan bertambahnya umur larva hingga 5 hari, selanjutnya meningkat tajam hingga larva berumur 7 hari dan kemudian menurun. Perkembangan enzim pencernaan larva ikan patin ini sejalan dengan perkembangan (diferensiasi) anatomi saluran pencernaan. Saluran pencernaan larva berisi Artemia, cacing dan plankton dengan jumlah yang semakin meningkat dengan bertambahnya umur larva.
Kata kunci: Enzim pencernaan, perkembangan, larva, ikan patin, Pangasius hypophthalmus
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| first_indexed | 2024-12-23T20:32:18Z |
| format | Article |
| id | doaj.art-a2c0dc33fa4b4ee69b406d067c1133ef |
| institution | Directory Open Access Journal |
| issn | 1412-5269 2354-6700 |
| language | English |
| last_indexed | 2025-02-16T18:21:23Z |
| publishDate | 2003-05-01 |
| publisher | Asosiasi Sains Akuakultur Indonesia |
| record_format | Article |
| series | Jurnal Akuakultur Indonesia |
| spelling | doaj.art-a2c0dc33fa4b4ee69b406d067c1133ef2025-01-24T18:06:08ZengAsosiasi Sains Akuakultur IndonesiaJurnal Akuakultur Indonesia1412-52692354-67002003-05-012110.19027/jai.2.13-203483PERKEMBANGAN ENZIM PENCERNAAN LARVA IKAN PATIN, Pangasius hypophthalmus sp.Irzal Effendi0. Widanarni1D. Augustine2Bogor Agricultural University, Department of AquacultureBogor Agricultural University, Department of AquacultureBogor Agricultural University, Department of AquacultureDevelopment of digestive enzymes; protease, lipase and amylase were observed in patin catfish, Pangasius hypophthalmus, larvae. The 1 day old larvae (day after hatching), with 3,37-3,97 mm length and 0,62-0,79 mg weight, were reared in aquarium 60x50x40 cm with stocking density of 20 fish/l. Larvae were fed Artemia dan tubificid worms 2-8 dan 7-15 days after hatching (dAH), respectively (schedule I); 2-6 and 5-15 dAH (schedule II); and 2-4 and 5-15 dAH (schedule III). Chlorella was ready to eat by larvae at the entirely rearing. For enzyme assay, larvae were sampled from each aquarium at stages of 1, 2, 3, 5, 7, 10 and 15 dAH. Protease and lipase activity were detected in digestive tract of 1 dAH larvae. Digestive enzymes development have a similar pattern in larvae for all feeding schedules. Protease activity decreased with the increasing of age until 3 dAH, then increased until the larvae reached 7 dAH, and sharply decreased until 10 dAH and then slowly decreased thereafter. Lipase activity tended to increase slowly with age up to 3 dAH, and increased sharply until 5 dAH, and then decreased sharply until 7 dAH before decreased again up to the end of rearing. Amylase activity in larvae increased slowly with the increasing of age up to 5 dAH, then increased sharply until 7 dAH, and decreased thereafter. In dimly lighted larvae, amylase activity decreased before increased up to 12 d AH, then decreased thereafter. The amount of food organisms in larval gut, body weight and length, and survival rate of larvae were also measured and discussed. Key Words: Digestive enzymes, development, larvae, patin catfish, Pangasius hypophthalmus ABSTRAKPenelitian ini bertujuan untuk mengetahui perkembangan enzim protease, lipase dan amilase saluran pencernaan larva ikan patin akibat perubahan skedul pemberian pakan. Larva ikan patin (panjang 3,77–3,97 mm dan bobot 0,62-0,79 mg) berumur 1 hari dipelihara di akuarium 60x50x40 cm dengan kepadatan 20 ekor/l. Larva diberi pakan dengan tiga skedul pemberian; skedul I, Artemia dan cacing diberikan ketika larva ikan patin berumur masing-masing 2-8 dan 7-15 hari; skedul II, Artemia 2-6 hari dan cacing 5-15 hari; skedul III, Artemia 2-4 hari dan cacing 5-15 hari. Chlorella diberikan sepanjang pemeliharaan larva. Contoh larva umur 1, 2, 3, 5, 7, 10 dan 15 hari diambil sebanyak 0,5 g setelah dipuasakan selama 4 jam. Asai protease, lipase dan amilase terhadap homogenate larva dilakukan dengan menggunakan masing-masing substrat kasein 1%, minyak kelapa sawit dan pati 1%. Anatomi, isi saluran pencernaan, pertumbuhan bobot dan kelangsungan hidup larva juga diamati. Tidak terdapat perbedaan pertumbuhan larva yang diberi pakan dengan skedul berbeda, namun larva yang diberi Artemia lebih lama (skedul I) memiliki kelangsungan hidup lebih baik. Larva ikan patin umur 1 hari setelah menetas ternyata sudah mengandung enzim protease dan lipase di saluran pencernaannya. Perkembangan enzim pencernaan memiliki pola yang hampir sama pada setiap skedul pemberian pakan. Aktivitas protease menurun pada larva umur 3 hari, selanjutnya meningkat tajam hingga larva umur 7 hari, kemudian menurun tajam hingga larva umur 10 hari dan akhirnya menurun landai. Aktivitas lipase meningkat lambat hingga larva umur 3 hari, kemudian meningkat tajam hingga larva umur 5 hari, selanjutnya menurun tajam hingga larva umur 7 hari dan akhirnya menurun landai. Aktivitas amilase semakin meningkat lambat dengan bertambahnya umur larva hingga 5 hari, selanjutnya meningkat tajam hingga larva berumur 7 hari dan kemudian menurun. Perkembangan enzim pencernaan larva ikan patin ini sejalan dengan perkembangan (diferensiasi) anatomi saluran pencernaan. Saluran pencernaan larva berisi Artemia, cacing dan plankton dengan jumlah yang semakin meningkat dengan bertambahnya umur larva. Kata kunci: Enzim pencernaan, perkembangan, larva, ikan patin, Pangasius hypophthalmus https://journal.ipb.ac.id/index.php/jai/article/view/3863 |
| spellingShingle | Irzal Effendi . Widanarni D. Augustine PERKEMBANGAN ENZIM PENCERNAAN LARVA IKAN PATIN, Pangasius hypophthalmus sp. Jurnal Akuakultur Indonesia |
| title | PERKEMBANGAN ENZIM PENCERNAAN LARVA IKAN PATIN, Pangasius hypophthalmus sp. |
| title_full | PERKEMBANGAN ENZIM PENCERNAAN LARVA IKAN PATIN, Pangasius hypophthalmus sp. |
| title_fullStr | PERKEMBANGAN ENZIM PENCERNAAN LARVA IKAN PATIN, Pangasius hypophthalmus sp. |
| title_full_unstemmed | PERKEMBANGAN ENZIM PENCERNAAN LARVA IKAN PATIN, Pangasius hypophthalmus sp. |
| title_short | PERKEMBANGAN ENZIM PENCERNAAN LARVA IKAN PATIN, Pangasius hypophthalmus sp. |
| title_sort | perkembangan enzim pencernaan larva ikan patin pangasius hypophthalmus sp |
| url | https://journal.ipb.ac.id/index.php/jai/article/view/3863 |
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